High-performance liquid chromatography of amino acids after derivatization with 9-isothiocyanatoacridine

Oravec, Peter; Podhradský, Dušan

doi:10.1016/0165-022x(94)00073-m

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…Pre- and postcolumn chemical derivatization has been proposed in an effort to increase the sensitivity of amino acid analysis. This has led to the development of several derivatizing reagents that can react with the amino and/or carboxylic acid group of the amino acid, each exhibiting certain advantages and limitations. − For example, Uutela et al compared the performance of three commonly used amino acid derivatization reagents and showed 2−60 times improvement in limits of detection as a result of amino acid derivatization . The main challenge facing many derivatization strategies is sample interference, particularly when nonspecific detectors are used.…”

mentioning

confidence: 99%

Sensitive and Rapid Method for Amino Acid Quantitation in Malaria Biological Samples Using AccQ•Tag Ultra Performance Liquid Chromatography-Electrospray Ionization-MS/MS with Multiple Reaction Monitoring

et al. 2009

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An AccQ•Tag Ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ•Tag -UPLC-ESI-MS/MS) method for fast, reproducible and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with photodiode array (PDA) detector was used for amino acid separation and detection. The method was developed and validated using amino acid standard mixtures containing acidic, neutral, and basic amino acids. For MS analysis, the optimum cone voltage implemented, based on direct infusion analysis of a few selected AccQ•Tag amino acids with multiple reaction monitoring, varied from 29-39 V, whereas the collision energy varied from 15-35 V. Calibration curves were built using both internal and external standardization. Typically, a linear response for all amino acids was observed at concentrations ranges of 3 × 10−3-25 pmol/μL. For some amino acids, concentration limits of detection were as low as 1.65 fmol. The coefficients of variation for retention times were within the ranges of 0.08-1.08%. The coefficients of variation for amino acid quantitation, determined from triplicate UPLC-MS/MS runs, were below 8% on the average. The developed AccQ•Tag-UPLC-ESI-MS/MS method revealed good technical and biological reproducibility when applied to P. falciparum and human red blood cells samples. This study should provide a valuable insight into the performance of UPLC-ESI-MS/MS for amino acid quantitation using AccQ•Tag derivatization.

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confidence: 99%

Sensitive and Rapid Method for Amino Acid Quantitation in Malaria Biological Samples Using AccQ•Tag Ultra Performance Liquid Chromatography-Electrospray Ionization-MS/MS with Multiple Reaction Monitoring

et al. 2009

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“…In accordance with the classical acid hydrolysis methods [19][20][21][22][23][24][25], leuprolide acetate was hydrolyzed [1] with 6N hydrochloric acid for 24 h at 110 • C, discarding alternative hydrolysis media such as alkali or alkyl sulphonic acids because these can react with amino acids like Arg and Ser. On the basis of that, to choice the adequate conditions for acid hydrolysis, before to handle the expensive leuprolide acetate sample, the course of the hydrolysis reaction was studied on the simple tripeptide Met-Leu-Phe acetate, under reflux with hydrochloric acid at different concentrations.…”

Section: Peptide Hydrolysismentioning

confidence: 99%

LC determination of leuprolide component amino acids in injectable solution by phanquinone pre-column derivatization labelling procedure

Gioia

Gatti

Minarini

2005

Journal of Pharmaceutical and Biomedical Analysis

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“…Among the common chromophoric reagents used for derivatizing AA for CE, some limitations in detecting the resulting derivatives have been reported. These include the AA derivatives from PITC that lack fluorescence property [15]; the derivatives from DNS-Cl are photosensitive and cover unfavorable byproducts [16,17]; the derivatives from OPA are unstable and on-column or postcolumn derivatization has to be used [18]; the derivatives from NDA give better stability than that from OPA, but the toxic cyanide is used in the derivatization; primary AA derivatized with fluorescamine gives two derivatives; the derivatives from FMOC give less fluorescence intensity in water than in organic solvent [19]; and FITC is unsuitable for derivatizing AA at trace levels because the resulting by-products interfere in the analysis [20]. Detailed appraisal of OPA, PITC, FMOC, and DNS-Cl in derivatizing AA for LC has been reported [18].…”

Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic capillary chromatographic method for the separation and quantitation of multiple amino acids as naphthoxy derivatives in pharmaceutical formulations

2006

Electrophoresis

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The MEKC method is described for the quantitative analysis of 17 amino acids (AA) in pharmaceutical products. The method is based on simply derivatizing the AA with (2-naphthoxy)acetyl chloride under mild conditions. The resulting derivatives were separated by MEKC with borate buffer (35 mM; pH 9.50) including 150 mM SDS at the applied voltage of 25 kV in an uncoated capillary (effective length, 40 cm) and monitored by UV at 230 nm. The detection limits of the amino acid derivatives were in the range of 3.0-8.0 microM (S/N = 3, injection 5.0 s, 6 895 Pa). The precision (RSD) and accuracy (relative error) of the method for intra- and interday analyses of the analytes are all below 5.2%. The amino acid derivatives are stable at room temperature for 33 h studied and the molar absorptivity of the alanine derivative (used as a model) is stable over a wide pH range of 3.00-12.00. This is favorable for monitoring the derivatives in various pH by CE or LC. Application of the method to the analysis of multiple AA in a liquid injection formulation proved satisfactory.

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High-performance liquid chromatography of amino acids after derivatization with 9-isothiocyanatoacridine

Cited by 8 publications

References 10 publications

Sensitive and Rapid Method for Amino Acid Quantitation in Malaria Biological Samples Using AccQ•Tag Ultra Performance Liquid Chromatography-Electrospray Ionization-MS/MS with Multiple Reaction Monitoring

Sensitive and Rapid Method for Amino Acid Quantitation in Malaria Biological Samples Using AccQ•Tag Ultra Performance Liquid Chromatography-Electrospray Ionization-MS/MS with Multiple Reaction Monitoring

LC determination of leuprolide component amino acids in injectable solution by phanquinone pre-column derivatization labelling procedure

Micellar electrokinetic capillary chromatographic method for the separation and quantitation of multiple amino acids as naphthoxy derivatives in pharmaceutical formulations

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