High-performance liquid chromatography of nalbuphine, butorphanol and morphine in blood and brain microdialysate samples: Application to pharmacokinetic/pharmacodynamic studies in rats

Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac

“…The proposed method could improve the sensitivity of Mor by more than 20 times compared to HPLC-DAD method [7,8] and native FL method [13][14][15]. Furthermore, it was comparable to those of HPLC-ECD [10] and -MS/MS method [20] published recently for determination of Mor in microdialysate.…”

“…For the determination of Mor, high performance liquid chromatography (HPLC) methods with UV or DAD detection [7,8], electrochemical detection (ECD) [9,10], mass spectrometry (MS) detection [11,12] and fluorescence detection (FL) [13][14][15][16] are widely used. Quite recently, HPLC methods combined with tandem MS/MS for very low-level quantification of Mor in biological samples have been described with sub-nanogram per milliliter of quantitation limit [17][18][19][20].…”

Relationship between plasma concentrations of morphine and its metabolites and pain in cancer patients

“…HPLC with UV detection is a common analytical method for these drugs in biological samples. However, many reports describe the need for multiple extractions and complex chromatographic systems to ensure reproducibility and resolution [19][20][21][22][23][24][25]. Recently, a method employing HPLC combined with electro spray ionization-tandem mass spectrometry (HPLC-ESI-MS-MS) for low level quantitation of MOR, M3G and M6G has been described [26].…”

Kinetic Spectrophotometry Method for the Determination of Morphine, Nalbuphine and Naltrexone Drugs in Bulk and Pharmaceutical Formulations

“…A few methods have been described to detect nalbuphine in pharmaceutical formulations; they include GC coupled to electron-capture detection, 26 or mass spectrometry, 27 HPLC with electrochemical detection, [28][29][30][31][32][33][34] and ion-selective electrode. 35 Although the GC methods, 26,27 are sensitive, they involved expensive equipments and time consuming samples preparation and are not easily available for regular drug monitoring.…”