High-performance liquid chromatography of phenylthiocarbamyl derivatives of amino acids and side-chain derivatized amino acids

Sensitive and Rapid Method for Amino Acid Quantitation in Malaria Biological Samples Using AccQ•Tag Ultra Performance Liquid Chromatography-Electrospray Ionization-MS/MS with Multiple Reaction Monitoring

“…Pre- and postcolumn chemical derivatization has been proposed in an effort to increase the sensitivity of amino acid analysis. This has led to the development of several derivatizing reagents that can react with the amino and/or carboxylic acid group of the amino acid, each exhibiting certain advantages and limitations. − For example, Uutela et al compared the performance of three commonly used amino acid derivatization reagents and showed 2−60 times improvement in limits of detection as a result of amino acid derivatization . The main challenge facing many derivatization strategies is sample interference, particularly when nonspecific detectors are used.…”