“…Methods for HPLC separation of steroids up to 1987 have been reviewed by Makin and Heftmann (1988). Reviews by many authors (Volin, 1995(Volin, , 2001Marwah et al, 2001;Shimada et al, 2001;Gorog, 2004) consider a number of HPLC separations of steroids in relation to clinical applications and sensitivity, respectively.…”