High-performance liquid chromatography on continuous polymer beds

Hjertén, Stellan; Liao, Jiali; Zhang, Rong

doi:10.1016/s0021-9673(00)91309-8

Cited by 667 publications

(345 citation statements)

References 6 publications

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“…The pore-size distribution and the porosity of the rod can be easily controlled by selecting the proper porogen and varying the volume ratio of porogen to monomer. Linear decreases in efficiency due to intraparticle w x diffusion at increasing flow-rates are not observed 22,43,[130][131][132][133][134][135][136][137][138] . A number of affinity-membrane cartridges are commercially available.…”

Section: Categories and Characteristics Of Affinity Membrane Cartridgesmentioning

confidence: 92%

Affinity membrane chromatography for the analysis and purification of proteins

Zou

Luo

Zhou

2001

Journal of Biochemical and Biophysical Methods

222

137

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Section: Categories and Characteristics Of Affinity Membrane Cartridgesmentioning

confidence: 92%

Affinity membrane chromatography for the analysis and purification of proteins

Zou

Luo

Zhou

2001

Journal of Biochemical and Biophysical Methods

222

137

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“…54,55 Fréchet and colleagues introduced macroporous monolithic polymeric materials based on poly-(glycidyl methacrylate-co-ethylene dimethacrylate) in which trypsins were immobilized. 56 This organic polymer-based bioreactor showed a variety of useful properties such as high flow-permeability, good biocompatibility, high enzyme stability, excellent pH stability, and fast mass transfer characteristics.…”

Section: 47mentioning

confidence: 99%

Protein Analysis Using a Combination of an Online Monolithic Trypsin Immobilized Enzyme Reactor and Collisionally-Activated Dissociation/Electron Transfer Dissociation Dual Tandem Mass Spectrometry

Hwang¹,

Cho²,

Kim³

et al. 2012

Bulletin of the Korean Chemical Society

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We demonstrated the combined applications of online protein digestion using trypsin immobilized enzyme reactor (IMER) and dual tandem mass spectrometry with collisionally activated dissociation (CAD) and electron transfer dissociation (ETD) for tryptic peptides eluted through the trypsin-IMER. For the trypsin-IMER, the organic and inorganic hybrid monolithic material was used. By employing the trypsin-IMER, the long digestion time could be saved with little or no sacrifice of the digestion efficiency, which was demonstrated for standard protein samples. For three model proteins (cytochrome c, carbonic anhydrase, and bovine serum albumin), the tryptic peptides digested by the IMER were analyzed using LC-MS/MS with the dual application of CAD and ETD. As previously shown by others, the dual application of CAD and ETD increased the sequence coverage in comparison with CAD application only. In particular, ETD was very useful for the analysis of highly-protontated peptide cations, e.g., ≥ 3+. The combination approach provided the advantages of both trypsin-IMER and CAD/ETD dual tandem mass spectrometry applications, which are rapid digestion (i.e., 10 min), good digestion efficiency, online coupling of trypsin-IMER and liquid chromatography, and high sequence coverage.

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“…Hjerten et al [59][60][61][62] prepared rod columns from swollen polyacrylamide gels. Polymerization can be carried out directly in the column [63][64][65][66][67] or membranes can be prepared from a suitable polymer, placed in a cartridge and used for rapid separations [68].…”

Section: Supports and Their Modificationmentioning

confidence: 99%

Stationary phases for peptide analysis by high performance liquid chromatography: a review

Štulı́k¹,

Pacáková²,

Suchánková³

et al. 1997

Analytica Chimica Acta

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A survey is given of modern stationary phases employed in high performance liquid chromatography (HPLC) analysis of peptides. The physico-chemical properties of peptides and their consequences for the selection and optimization of the separation system are briefly discussed, followed by a summary of the approaches to the selection and characterization of stationary phases. The properties and applicability of various stationary phases are then critically reviewed, including aspects such as size-exclusion, ion-exchange, reversed-phase, hydrophobic-interaction, affinity and chiral systems, as well as some specialized separation techniques. Emphasis is placed on the most recent literature. 0 1997 Elsevier Science B.V.

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High-performance liquid chromatography on continuous polymer beds

Cited by 667 publications

References 6 publications

Affinity membrane chromatography for the analysis and purification of proteins

Affinity membrane chromatography for the analysis and purification of proteins

Protein Analysis Using a Combination of an Online Monolithic Trypsin Immobilized Enzyme Reactor and Collisionally-Activated Dissociation/Electron Transfer Dissociation Dual Tandem Mass Spectrometry

Stationary phases for peptide analysis by high performance liquid chromatography: a review

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