High‐performance liquid chromatography study of water‐soluble ternary polyacrylamide‐metal‐protein complexes

High performance liquid chromatography study of water‐soluble complexes and covalent conjugates of polyacrylic acid with bovine serum albumin

“…BSA is characterized by a high content of cystine (35) and the charged amino acids, aspartic (41) and glutamic (58) acids, lysine (60) and arginine (26). Based on hydrodynamic [48][49][50] and low-angle X-ray scattering 51 serum albumin was postulated to be an oblate ellipsoid with dimensions of 140 Â 40 Å 2 .…”

“…Soluble polymer-protein complexes were studied by a wide range of methods, which are well known in colloid and polymer chemistry. 13,[22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] Interaction of polyelectrolytes with proteins in aqueous solutions is found to be sensitive to pH and ionic strength, as well as be dependent on isoelectric points of proteins. 14,37,40 Polymer-protein complexes form as a result of the electrostatic interaction of polyion chains with the oppositely charged groups of the protein molecules.…”

“…The complex formation between PEs and proteins has been as subjects of numerous researches. Soluble polymer–protein complexes were studied by a wide range of methods, which are well known in colloid and polymer chemistry 13, 22–39. Interaction of polyelectrolytes with proteins in aqueous solutions is found to be sensitive to pH and ionic strength, as well as be dependent on isoelectric points of proteins 14, 37, 40.…”

The Formation of Polycomplexes of Poly(Methyl Vinyl Ether-Co-Maleic Anhydride) and Bovine Serum Albumin in the Presence of Copper Ions

“…Investigation of water soluble and insoluble complexes of polymer with protein in the presence of metal ions has important applications in various areas [1][2][3][4][5][6][7][8][9][10][11][12][13][14] . In recent studies, it has been shown that metal (M) ions (Cu 2+ , Zn 2+ , Fe 2+ ) which are complexed with functional polymers have an important role in biological processes 2, [15][16][17][18][19][20][21][22][23] .…”

Flow injection-capillary electrophoresis frontal analysis method for the study of the interactions of a series of drugs with human serum albumin

“…A variety of methodologies such as fluorescence spectroscopy (FLU) [6], equilibrium dialysis (ED) [7,8], HPLC [9,10], flow injection chemiluminescence or sequential injection analysis [11,12], mass spectrometry (MS) [13] and CE [4,14] have been developed to evaluate drug-protein interactions. FLU is a simple method and has advantages such as high sensitivity and small sample requirement, but it can only be applied in a limited field.…”