High performance liquid chromatography with chemiluminescence detection of methamphetamine and its related compounds using 4‐(N,N‐dimethylaminosulphonyl)‐7‐fluoro‐2,1,3‐benzoxadiazole

Abstract

A high performance liquid chromatographic assay of methamphetamine (MP) and its related compounds, i.e. ephedrine (EP), norephedrine (NE), p-hydroxymethamphetamine (p-HMP), p-hydroxyamphetamine (p-HAP) and amphetamine (AP), with peroxyoxalate chemiluminescence detection has been developed. 4-(N,N-Dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F) was used as a fluorescent labeling reagent. A mixture of hydrogen peroxide and bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate in acetonitrile was used as a postcolumn chemiluminogenic reagent. DBD derivatives of MP and its related compounds were separated by a gradient elution with acetonitrile and 0.01 M imidazole buffer (pH 7.0) within 65 min. The detection limits (S/N = 3) for the proposed method for MP, AP, EP, NE, p-HMP and p-HAP were 27, 100, 40, 133, 25 and 133 fmol on column, respectively. The recoveries of these compounds with normal urine samples were 87.4-106.4%. The method was successfully applied to the assay of MP and its metabolites in urine samples from MP addicts. A good linear correlation for the resulted amounts of MP or AP between the proposed method and gas chromatography was obtained (r = 0.993 for MP or 0.991 for AP).

Identification of reaction products of methamphetamine and hydrogen peroxide in hair dye and decolorant treatments by high‐performance liquid chromatography/mass spectrometry

“…Utilizing dansyl chloride as a fluorescent reagent, MA and its five metabolites in urine were determined (Hayakawa et al, 1993). Other derivatization reagents for the simultaneous determination of MA and its metabolites in HPLC/CL and /FL include N-(4-aminobutyl)-Nethyl-isoluminol (Nakashima et al, 1991) and 4-(N,Ndimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (Nakashima et al, 1992;Sato et al, 1997).…”

Determination of Stimulants in a Single Human Hair Sample by High-Performance Liquid Chromatographic Method with Chemiluminescence Detection

“…Utilizing the former derivatization method, MA and its five metabolites in urine were separately determined (Hayakawa et al, 1993). N-(4-Aminobuty1)-N-ethyl-isoluminol (Nakashima et al, 1991) and 4-(NJV-dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (Nakashima et al, 1992) were also used as other derivatization reagents for the simultaneous determination of MA and its metabolites. In this study, we applied the CL-HPLC method using dansyl derivatives for the sensitive determination of MA and AP in hair samples and confirmed that its sensitivity was higher than that of GCMS using selective ion monitoring (SIM).…”

An Invitation to Bio-Analytical Chemistry

“…2,3 It was applied to the ultra trace analysis of catecholamines 26 , amino acids [27][28][29][30] , steroids [31][32][33] and drugs. 3,[34][35][36][37][38][39][42][43][44][45][46] …”