High-performance liquid chromatography with non-aqueous solvents Relative elution strength on a polystyrene—divinylbenzene column

Chambers, Thomas K; Fritz, James S.

doi:10.1016/0021-9673(95)01023-8

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…Typically, good solvents of high elutropic strength toward PS-DVB materials are (in order of strength) MeCl 2 > EtOAc > MeCN > MeOH. Dimethyl sulfoxide, DMSO, and isopropyl alcohol, IPA, although good solvents, are usually quite weak mobile phases .…”

Section: Resultsmentioning

confidence: 99%

“…Deformed and noisy peaks were obtained as a rule, due to the presence of air/N 2 in the trap, and therefore, any precise calculations based on the peak area were not attempted. Typically, good solvents of high elutropic strength toward PS-DVB materials are (in order of strength 20 ) MeCl 2 > EtOAc > MeCN > MeOH. Dimethyl sulfoxide, DMSO, and isopropyl alcohol, IPA, although good solvents, are usually quite weak mobile phases.…”

Section: Stationary (Trapping) Phase Performance Comparisonsmentioning

confidence: 99%

See 1 more Smart Citation

Generic Compound Isolation Using Solid-Phase Trapping as Part of the Chromatographic Purification Process. Part 1. Proof of Generic Trapping Concept

et al. 2006

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Section: Resultsmentioning

confidence: 99%

Section: Stationary (Trapping) Phase Performance Comparisonsmentioning

confidence: 99%

Generic Compound Isolation Using Solid-Phase Trapping as Part of the Chromatographic Purification Process. Part 1. Proof of Generic Trapping Concept

et al. 2006

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“…2. A rectilinear correlation would indicate similarity in terms of selectivity [17]. From the equation of the trendline, superior retention between columns can be read in terms of a slope deviating from unity.…”

Section: Data Acquisition and Handlingmentioning

confidence: 99%

Broad‐spectrum separations in metabolomics using enhanced polar LC stationary phases: A dedicated evaluation using plant metabolites

t’Kindt

Alaerts

Heyden

et al. 2007

J of Separation Science

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Original PaperBroad-spectrum separations in metabolomics using enhanced polar LC stationary phases: A dedicated evaluation using plant metabolitesIn metabolomics, major efforts are invested in the development of suitable analytical approaches. A tendency towards the use of LC-MS is nowadays very obvious. A great majority of the metabolites of interest are polar to highly polar in nature. We focus on the so-called , extended polarity' reversed LC phases, developed specifically to allow better retention characteristics for polar compounds. Several of these phases (Atlantis dC18, Inertsil ODS-3, Zorbax XDB, Alltima HP C18) are tested for different column dimension variations (0.5, 1.0, 2.1 mm id) in a specific LC-MS metabolomics setting. Important chromatographic and mass spectrometric quality parameters such as capacity factor, separation efficiency, peak symmetry, sensitivity, and mass accuracy are taken into account. All phases show adequate retention of polar compounds and also perform well with highly aqueous mobile phase compositions. On comparing 1.0 and 2.1 mm id columns, it is clear that the potential gain in sensitivity is not achieved. Using a Lockspraym device, accurate mass measurement with a Q-TOF micro is feasible within a mass range of 12 ppm if signal intensities of compound and lockmass are equated. Finally, the extended polarity RP approach in metabolomics experiments is endorsed using real plant extracts.

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Influence of solvent effects on retention for a porous polymer sorbent in reversed phase liquid chromatography

Bolliet

Poole

1997

Chromatographia

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SummaryThe influence of acetonitrile, methanol and isopropanol as retention selectivity modifiers in reversed phase liquid chromatography on a poly(styrene-divinylbenzene) macroporous polymer sorbent (PLRP-S) is evaluated using the solvation parameter model. Retention results from a combination of adsorption and partitioning and is influenced by the equilibrium absorption of organic solvent by the polymer from the mobile phase. The sorption of solutes is dominated by the ease of cavity formation in or on the solvated sorbent, with a small contribution from lone pair-lone pair electron interactions. All polar interactions, such as dipole-type and hydrogenbond formation, are more favorable in the mobile phase and reduce retention. Changes in the uptake of organic solvent from the mobile phase affect kinetic properties of the column such as band broadening and porosity as well as retention. The PLRP-S solvated sorbent is suitable for solid-phase extraction and is more retentive than typical silica-based, bonded phase sorbents for extraction from water. As a surrogate system for estimating solute lipophilicity and biological activity through retention-property correlations it provides a poor fit for hydrogen-bond acid solutes and is too dipolar/polarizable to fit some models.

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High-performance liquid chromatography with non-aqueous solvents Relative elution strength on a polystyrene—divinylbenzene column

Cited by 10 publications

References 10 publications

Generic Compound Isolation Using Solid-Phase Trapping as Part of the Chromatographic Purification Process. Part 1. Proof of Generic Trapping Concept

Generic Compound Isolation Using Solid-Phase Trapping as Part of the Chromatographic Purification Process. Part 1. Proof of Generic Trapping Concept

Broad‐spectrum separations in metabolomics using enhanced polar LC stationary phases: A dedicated evaluation using plant metabolites

Influence of solvent effects on retention for a porous polymer sorbent in reversed phase liquid chromatography

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