High performance liquid chromatography with ultraviolet detection used for laboratory routine determination of fluvoxamine in human plasma

Abstract

<jats:p> Fluvoxamine is an antidepressant drug introduced into the clinic in 1986. It acts by selectively inhibiting neuronal serotonin recapture. It can be quantified by several meth ods, including high performance liquid chromatography. The HPLC method used so far needs special equipment and has poor sensitivity. The technique is difficult and time consuming. An easier, quicker and more sensitive HPLC assay for the routine determination of fluvoxamine in human plasma has therefore been developed. </jats:p><jats:p> After alkalinisation and direct extraction by a mixture of n-hexane-isoamylic alcohol 985: 15 (v/v) of plasma samples, the organic phases were further extracted by HCl 0.1 N. </jats:p><jats:p> Thirty μL of the final extract (with loxapine as internal standard) were injected directly into a C-8 column with a mobile phase consisting of 370 mL acetonitrile, 0.4 mL diethylamine, 630 mL of distilled water, 25 mL pic B5. UV detection at 254 nm was used. </jats:p><jats:p> The whole process was completed in 40 min. The detec tion limit was 10 ng mL-1. No interference was found either with several benzodiazepines or with antidepres sant drugs commonly associated during treatments. </jats:p>

High-Performance Liquid Chromatographic Determination of Fluvoxamine and Fluvoxamino Acid in Human Plasma

“…Several papers have described the HPLC method for the determination of fluvoxamine in plasma samples using a C8 stationary phase 14,[17][18][19] and C18 stationary phase. 13,16 We think, from our present results, that the C8 and C18 stationary phase are not necessarily suitable for the simultaneous analysis of fluvoxamine and fluvoxamino acid, because great opposite changes of k′ values were observed at higher pH in mobile phase on C8 and C18 stationary phase.…”

“…[13][14][15][16][17] Two papers have reported inline automatic solid phase extraction using C8 clean-up column. 18,19 Our simple solid-phase extraction method, an efficient combination system in HPLC determination method, could remove interfering substances.…”

“…Fluvoxamino acid is the main primary metabolite in humans. 4 Therefore, the monitoring of fluvoxamino acid on the drug interaction study of fluvoxamine will be able to detect slight changes of fluvoxamine metabolism by co-administered drug in healthy volunteers and/or patients.The pharmacokinetics of fluvoxamino acid in human has not yet been studied sufficiently.Methods for the fluvoxamine by GC-MS, 12 HPLC with liquidliquid extraction of plasma samples [13][14][15][16][17] and HPLC with solidphase in-line extraction 18,19 were described in previous papers. The liquid-liquid extraction methods are not completely satisfactory because they are time-consuming and require a tedious procedure.…”

“…Methods for the fluvoxamine by GC-MS, 12 HPLC with liquidliquid extraction of plasma samples [13][14][15][16][17] and HPLC with solidphase in-line extraction 18,19 were described in previous papers. The liquid-liquid extraction methods are not completely satisfactory because they are time-consuming and require a tedious procedure.…”

Electroanalytical study of fluvoxamine

“…Most FVX analysis is performed by high-perfor-mance liquid chromatography (HPLC) coupled with spectrometric detectors, ultraviolet/diode array [4][5][6][7][8][9][10][11][12][13][14][15][16][17], mass spectrometric [18,19] and fluorimetric [20][21][22], for analysis of pharmaceuticals [8] and human serum or plasma [4][5][6][7][10][11][12][13][14][15][16][17][18][19][20][21][22]. This widespread usage of HPLC methods is justified by the high sensitivity and the low limit of detection usually obtained and the possibility of simultaneous analysis of the drug and its metabolites, which increases its use even further.…”