High-performance thin layer chromatographic method for quantitative determination of curcuminoids in Curcuma longa germplasm

Paramasivam, M.; Poi, Rajlakshmi; Banerjee, Hemanta; Bandyopadhyay, A. K.

doi:10.1016/j.foodchem.2008.07.051

Cited by 133 publications

(78 citation statements)

References 16 publications

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“…Detailed knowledge about genetic relationships among wild and cultivated species of Curcuma will enhance the utilization value of wild species for any future study. A few studies based on morphological, anatomical, and biochemical characterization of Curcuma species and cultivars have been attempted earlier [34][35][36][37][38]. Relying much on the morphological characters alone in species delimitation has its own limitations since they are not always completely representative of the genetic structure [39].…”

Section: Discussionmentioning

confidence: 99%

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

et al. 2011

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Molecular genetic fingerprints of nine Curcuma species from Northeast India were developed using PCR-based markers. The aim involves elucidating there intra- and inter-specific genetic diversity important for utilization, management, and conservation. Twelve random amplified polymorphic DNA (RAPD), 19 Inter simple sequence repeats (ISSRs), and four amplified fragment length polymorphism (AFLP) primers produced 266 polymorphic fragments. ISSR confirmed maximum polymorphism of 98.55% whereas RAPD and AFLP showed 93.22 and 97.27%, respectively. Marker index and polymorphic information content varied in the range of 8.64-48.1, 19.75-48.14, and 25-28 and 0.17-0.48, 0.19-0.48, and 0.25-0.29 for RAPD, ISSR, and AFLP markers, respectively. The average value of number of observed alleles, number of effective alleles, mean Nei's gene diversity, and Shannon's information index were 1.93-1.98, 1.37-1.62, 0.23-0.36, and 0.38-0.50, respectively, for three DNA markers used. Dendrograms based on three molecular data using unweighted pair group method with arithmetic mean (UPGMA) was congruent and classified the Curcuma species into two major clusters. Cophenetic correlation coefficient between dendrogram and original similarity matrix were significant for RAPD (r = 0.96), ISSR (r = 0.94), and AFLP (r = 0.97). Clustering was further supported by principle coordinate analysis. High genetic polymorphism documented is significant for conservation and further improvement of Curcuma species.

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Section: Discussionmentioning

confidence: 99%

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

et al. 2011

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“…Turmeric (Curcuma longa L.) is a member of the Zingiberaceae family and is cultivated in tropical and subtropical regions around the world (Paramasivam, Poi, Banerjee, & Bandyopadhyay, 2009). The powder is used extensively as a colouring and flavouring agent in curries and mustards.…”

Section: Introductionmentioning

confidence: 99%

Separation of an effective fraction from turmeric against Streptococcus mutans biofilms by the comparison of curcuminoid content and anti-acidogenic activity

Pandit

Kim

et al. 2011

Food Chemistry

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“…They are of the opinion that LC-MS/MS method is more suitable for trace analysis of curcumin, while NMR technique for rapid analysis. While Paramasivam et al [19] have quantified the curcuminoids by HPTLC method citing its advantages such as shorter analysis time and minimal sample preparation. Jayaprakasha et al [10] employed improved HPLC method for the determination of curcuminoids.…”

Section: Resultsmentioning

confidence: 99%

Estimation of Curcuminoids in Curcuma Karnatakensis (White Turmeric) - An Endemic Taxon

Kannan

2017

Asian J Pharm Clin Res

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Objective: To estimate the contents of curcuminoids in two samples of Curcuma karnatakensis collected from different localities.Methods: Quantification of curcuminoids was estimated by ultra-high performance liquid chromatography-mass spectrometry/selected reaction monitoring (UHPLC-MS/SRM) method in two samples of C. karnatakensis for the first time. Fine powder of rhizomatous rootstocks of two plant samples collected from different habitats were served as Samples A and B. The MS used for the metabolite analysis is a Vantage TSQ triple stage quadrupole MS equipped with heated electrospray ionization. The MS is coupled with an Agilent 1290 infinity UHPLC system. A stock solution of curcuminoid standard was prepared by dissolving 5 mg of standard in 1 mL of methanol. Seven different concentrations of standard (0.15-10 ng on column) were injected for the UHPLC-MS/SRM analysis. Separations were performed using a C-18 column with a flow rate of 0.2 mL/minute. Results:Contents of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found to be varied in two samples and lowest than any other species of Curcuma studied. Variation in the contents may be due to their different habitats in which they are growing. Conclusion:The present attempt of analyzing the contents of curcuminoids in this endemic taxon for the first time will provide the basis for further pharmacological analysis to authenticate the efficacy of these active principles as the curcuminoids are known for varied pharmacological activities.

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High-performance thin layer chromatographic method for quantitative determination of curcuminoids in Curcuma longa germplasm

Cited by 133 publications

References 16 publications

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

Separation of an effective fraction from turmeric against Streptococcus mutans biofilms by the comparison of curcuminoid content and anti-acidogenic activity

Estimation of Curcuminoids in Curcuma Karnatakensis (White Turmeric) - An Endemic Taxon

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