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A simple high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous determination of the pharmacologically important quinazoline alkaloids vasicine and vasicinone in Adhatoda vasica. The assay combines the separation and quantification of the analytes on silica gel 60 GF254 HPTLC plates with visualisation under UV and scanning at 270 and 281 nm. Using this technique, the alkaloidal content of different parts of the title plant have been determined.

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A modified QuEChERS method was developed for the determination of 64 pesticides in apple, guava andbanana using GC-MS and LC-MS/MS. Out of sixty-four pesticides, 39 (32 insecticides + 7 herbicides) were analysed by GC-MS and others were analysed in LC-MS/MS. The efficiency of methanol, ethyl-acetate and acetonitrile as extracting solvents were checked. Among these, acetonitrile gave the highest recovery. d-SPE clean-up was done for apple and guava using PSA, whereas PSA + GCB was used for banana to remove heteropolysaccharides and carotenoids. The method was validated as per SANTE/11813/2017 guidelines. The LOD was in the range of 0.002-0.04 mg kg −1 and LOQ was 0.005-0.11 mg kg −1. Average recovery ranged from 80 to 120% with RSD ≤ 20% and RPD % of precision ≤ 20%. The expanded uncertainty was ≤ 20%. The validated method was applied to check the real samples of apple, guava and banana collected from markets of four different districts of West Bengal, India.

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