High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants

Reich, Eike; Schibli, Anne

doi:10.1055/b-002-66241

Cited by 152 publications

(13 citation statements)

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“…The chromatogram indicates three components of analyte stability: in the extract solution and on the TLC plate, during chromatography, and after derivatization. The stability test method referred to Reich and Schibli (Reich and Schibli 2007) with modifications. The stability of the analyte in the extract solution and on the TLC plate was tested in parallel using a 10 x 10 cm TLC plate.…”

Section: Stability Testmentioning

confidence: 99%

“…After the first elution, it was dried, rotated 90° counterclockwise, and re-eluted using a new mobile phase. A sample is stable during chromatography if the zones form a diagonal line connecting the initial point (lower right corner) and the intersection of the two elution results (Reich and Schibli 2007).…”

Section: Stability Testmentioning

confidence: 99%

“…The vanillinsulfuric acid reagent was sprayed onto the plate, then the results were observed after 5, 10, 30, and 60 minutes. An analyte is considered stable after derivatization if the Rf value does not vary throughout the 60-minute observation (Reich and Schibli 2007).…”

Section: Stability Testmentioning

confidence: 99%

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TLC-fingerprinting and chemometrics for identification of Curcuma xanthorrhiza from different geographical origins in Indonesia

KARTINI,

SABATINI,

HARIDSA

et al. 2023

Biodiversitas

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Abstract. Kartini K, Sabatini SS, Haridsa NM, Jayani NIE, Setiawan F, Hadiyat MA. 2023. TLC-fingerprinting and chemometrics for identification of Curcuma xanthorrhiza from different geographical origins in Indonesia. Biodiversitas 24: 6557-6566. Geographical origin is an important parameter that influences the quality of herbal medicine. The fingerprint of herbal plants is expressed as chemical compounds contributing to said quality. Curcuma xanthorrhiza, popular as java turmeric, is extensively used in Indonesian traditional medicine. This study aimed to validate and develop a Thin Layer Chromatography (TLC) fingerprint of C. xanthorrhiza, followed by chemometric techniques to classify C. xanthorrhiza rhizomes from 15 regions in Indonesia. Under selected TLC conditions (i.e., stationary phase: TLC plate Silica gel 60 GF254, mobile phase: dichloromethane, chloroform, and ethanol (10: 10: 1), detection: vanillin-sulfuric acid reagent), C. xanthorrhiza produced five zones of the compound. Extracts were stable both on the TLC plate and in the extract solution during chromatography and within 60 minutes after derivatization. In terms of precision, the chromatogram meets the requirement of intraday precision. Chemometric analysis with Principal Component Analysis (PCA) and Cluster Analysis (CA) showed that the samples of C. xanthorrhiza from 15 regions in Indonesia were grouped into five clusters based on the similarity of the chemical compounds, namely cluster 1 (Tawangmangu, Bangkalan, Kediri, and Surabaya); cluster 2 (Batu, Sragen, Tulungagung, Pasuruan, Blitar, and Lombok Tengah); cluster 3 (Ngawi); cluster 4 (Gresik); and cluster 5 (Bojonegoro, Banyuwangi, and Palangkaraya). The TLC fingerprinting with chemometrics on C. xanthorrhiza rhizome is useful for quality control based on geographic origin and authenticity identification.

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Section: Stability Testmentioning

confidence: 99%

Section: Stability Testmentioning

confidence: 99%

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TLC-fingerprinting and chemometrics for identification of Curcuma xanthorrhiza from different geographical origins in Indonesia

KARTINI,

SABATINI,

HARIDSA

et al. 2023

Biodiversitas

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“…Использование современного оборудования представляет возможность сделать метод полуавтоматическим и программируемым. Идентификация с помощью метода ВЭТСХ включает в себя следующие стадии: пробоподготовка (получение извлечения из лекарственного растительного сырья), нанесение, элюирование, дериватизация (при необходимости), оценка хроматограмм (классическая денситометрия, сканирование), документирование, обработка снимков, архивирование [12]. Основной категорией риска при проведении анализа является ручной перенос пластинки с этапа на этап [1].…”

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“…Детекция прово-дится с использованием различных длин волн, что обеспечивает получение более полного профиля исследуемого объекта. Постхроматографическая дериватизация может осуществляться разнообразными реагентами и обеспечивает одновременную обработку стандартов и образцов в одинаковых условиях [12].…”

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Using high performance thin layer chromatography for the detection of pharmacologically active secondary metabolites in Empetrum nigrum L.

Ponkratova

Whaley

Лужанин

et al. 2021

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. The article presents the results of the detection of pharmacologically active secondary metabolites in black crowberry Empetrum nigrum L. using the method of high performance thin layer chromatography (HPTLC).Aim. To show the efficiency of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites in promising medicinal plant species.Materials and methods. HPTLC analysis was carried out on a CAMAG device (Switzerland), using MERCK HPTLC silica gel 60 F154, 20 × 10 cm plates. For the evaporation of the samples, a Heidolph vacuum rotary evaporator (Germany) was used. The aerial parts (shoots) of Empetrum nigrum were harvested next to St. Petersburg State Chemical and Pharmaceutical University (SPCPU) nursery garden of medicinal plants (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in August 2019.Results and discussion. In the course of the research, four fractions from the aerial parts of Empetrum nigrum were obtained: hexane, dichloromethane, butanol, and water. Then, these fractions were investigated by HPTLC in two solvent systems – n-butanol : acetic acid : water (BAW) (4 : 1 : 2) and hexane : dichloromethane : methanol (HDM) (1 : 2 : 0.5). After scanning densitometric analysis of the plates eluted in the HDM system, it was revealed, that the hexane and dichloromethane fractions have a similar composition and contain the greatest amount of compounds, compared to the butanol and water fractions, and in the BAW system, it was found, that the butanol fraction contains the greatest variety of metabolites. As a result of UV spectroscopy, it was found, that the main groups of compounds contained in the hexane and dichloromethane fractions are derivatives of chalcones, dihydrochalcones, bibenzyls and 9,10-dihydrophenanthrenes. While in the butanol fraction, the main groups of secondary metabolites were derivatives of flavonoids and tanninsConclusion. The data obtained allow us to note the efficiency, speed and simplicity of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites of promising medicinal plant species.

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Thin Layer Chromatography

Reich¹,

Widmer²

2012

Ullmann's Encyclopedia of Industrial Chemistry

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High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants

Cited by 152 publications

References 0 publications

TLC-fingerprinting and chemometrics for identification of Curcuma xanthorrhiza from different geographical origins in Indonesia

TLC-fingerprinting and chemometrics for identification of Curcuma xanthorrhiza from different geographical origins in Indonesia

Using high performance thin layer chromatography for the detection of pharmacologically active secondary metabolites in Empetrum nigrum L.

Thin Layer Chromatography

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