High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

Abstract

Objective: An attempt has been made to study phytoconstituents and High-Performance Thin Layer Chromatography (HPTLC) fingerprinting pattern of leaf/stem/root methanol extracts of Avicennia marina. Methods:The phytochemical screening was done by standard biochemical methods and standard optimized HPTLC densitometry determination was performed under two different energy zones [under UV-254 nm and under 540 nm after derivatization at sample size-10 µl, temperature = 25.8±0.3 °C (constant) and relative humidity = 86±1% (constant) in optimized solvent system].Results: Phytochemical analysis confirmed the presence of phytochemicals in the leaf, stem, and root of the plant. Under UV-254 nm energy range, the leaf, stem and root extract showed the presence of 8, 6 and 5 components respectively and under 540 nm energy zone after derivatization, the leaf, stem and root extract revealed the presence of 7, 5 and 5 components, respectively and specific marker components with very high concentration (under UV-254 nm; 3, 2, 2 in leaf, stem, and root respectively and under 540 nm after ASR treatment; 2, 1, 2 in leaf, stem, and root respectively) were detected among them. Conclusion:The preliminary phytochemical test results elucidated Avicennia marina leaf, stem, and root as a hug reservoir of various class of phytochemicals and metabolites. The results obtained by HPTLC fingerprinting method found to be acceptable as a quick, reliable, accurate and economical for identification and authentication of Avicennia marina mangrove plant and useful to differentiate this mangrove species from other similar mangroves, in a single TLC plate run. This serves as a biochemical marker pattern for leaf, stem and root parts of this mangrove and for its diverse phytoconstituents.

Study of Antimicrobial Potentiality of Eight Different Mangrove Species and Development of Their Intra-Relationship Through Cluster Analysis

“…Shanmugapriya et al [19] showed that the saponin content is present in leaf of A. marina and absent in leaf of A. officinalis which was collected from Parangipettai, Tamil Nadu, India. Unlike the result, this study found that the saponin content in leaves of A. marina is totally absent, which is supported by another study [20]. However, this study supports the absence of saponin in leaf of A. officinalis.…”

Standardisation and Quality Control Parameters of <i>Phalatrikadi Ghana Vati</i> – An <i>Ayurvedic</i> Formulation

“…HPTLC is becoming a critical and indispensable analytical technique for the standardisation of herbal drugs. A unique identification of the product can obtained by optimising the HPTLC 19 . Ayurveda formulations are composed of multiple herbal ingredients possessing a cocktail of numerous phytoconstituents.…”

HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF Β-Sitosterol FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

“…HPTLC is used to assess, compare, and quantify the reservoirs of secondary metabolites in different plants and also can be used in fingerprinting as conducted by Dawane and Fulekar [1] in which the results obtained by HPTLC fingerprinting method were found reliable, accurate, and economical for identification and authentication of Avicennia marina mangrove species from other similar mangroves, in a single TLC plate run.…”