High-Performance Thin-Layer Chromatography Hyphenated with Microchemical and Biochemical Derivatizations in Bioactivity Profiling of Marine Species

Abstract

Marine organisms produce an array of biologically active natural products, many of which have unique structures that have not been found in terrestrial organisms. Hence, marine algae provide a unique source of bioactive compounds. The present study investigated 19 marine algae and one seagrass collected from Torquay beach, Victoria, Australia. High-performance thin-layer chromatography (HPTLC) hyphenated with microchemical (DPPH•, p-anisaldehyde, and Fast Blue B) and biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to evaluate antioxidant activity, presence of phytosterols and phenolic lipids, α-amylase and AChE inhibitory activities of extract components. Significant α-amylase and AChE inhibitory activities were observed in samples 2, 6, 8 and 10. Antioxidant activities in the samples were found to be correlated to phytosterol content (R2 = 0.78), but was not found to be related to either α-amylase or AChE inhibitory activities. α-Amylase inhibitory activities were correlated to AChE inhibition (R2 = 0.77) and attributed to the phytosterol content, based on the similar peak position in the chromatograms with the β-sitosterol chromatogram. Samples 1, 8, and especially sample 20, were found to contain phenolic lipids (alkyl resorcinol derivatives) with significant antioxidant activities. The results suggest that these marine species have a significant number of bioactive compounds that warrant further investigation.

HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts

“…The ethyl acetate extract from nonfermented dried leaves was compared with ethyl acetate extracts from fermented dried and fermented fresh rosemary leaves, in terms of antioxidant activity, total phenolic content, and extraction of natural products, via HPTLC screening analysis. Ethyl acetate was selected as the most efficient solvent for extraction of the widest range of polyphenolics and phytosterols [ 19 ]. It is a moderately polar solvent that has the advantages of being volatile, relatively non-toxic, and non-hygroscopic.…”

Is Our Natural Food Our Homeostasis? Array of a Thousand Effect-Directed Profiles of 68 Herbs and Spices

“…The latest α-amylase inhibition method, which used immersion of the plate into enzyme and substrate solutions ( Agatonovic-Kustrin and Morton, 2017 ; Agatonovic-Kustrin et al, 2019 ), was adjusted and transferred to a piezoelectric spraying procedure, in which the enzyme solution (62.5 U/mL in sodium acetate buffer) was sprayed onto the chromatogram (1 ml, red nozzle, level 5), followed by 30 min incubation at 37°C. As substrate 2%-soluble starch solution was sprayed onto the wet plate (0.5 ml, red nozzle, level 5).…”

Chemical constituents of Launaea sarmentosa roots

“…This spot was visualized yellow or blue-violet when sprayed with KMnO4 or with p-anisaldehyde/ sulfuric acid stainning solutions, respectively implying the triterpene nature of the spot. [11] This observation was supported by its 1 H and 13 Silica gel column chromatography eluted by different solvents failed to separate these triterpenes. However, literature showed that by changing the functional groups, the polarity of these compounds could be altered.…”