High‐Performance Thin‐Layer Chromatography–Mass Spectrometry for Analysis of Small Molecules

Morlock, Gertrud E.

doi:10.1002/9781118180730.ch49

Cited by 7 publications

(8 citation statements)

References 103 publications

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“…Eight different assays were selected and applied, which directly pointed to individual radical scavenging compounds (antioxidants), antibacterials against Gram-negative and Gram-positive bacteria as well as enzyme inhibitors against α-glucosidase, β-glucosidase, tyrosinase, AChE and β-glucuronidase. A plate prewashing was recommended [ 46 , 47 ] to obtain clear signal responses in each assay. Instead of the commonly used automated immersion of the plate, the assay solutions and suspensions were cost-efficiently applied by piezoelectric spraying, which required only few milliliters and avoided a zone shift or blurring of the main flavonoids in the tea samples when the plate was pulled out [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Effect-Directed Profiling of Powdered Tea Extracts for Catechins, Theaflavins, Flavonols and Caffeine

et al. 2021

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The antioxidative activity of Camelia sinensis tea and especially powdered tea extracts on the market, among others used as added value in functional foods, can considerably vary due to not only natural variance, but also adulteration and falsification. Thus, an effect-directed profiling was developed to prove the functional effects or health-promoting claims. It took 3–12 min per sample, depending on the assay incubation time, for 21 separations in parallel. Used as a fast product quality control, it can detect known and unknown bioactive compounds. Twenty tea extracts and a reference mixture of 11-bioactive compounds were investigated in parallel under the same chromatographic conditions by a newly developed reversed phase high-performance thin-layer chromatographic method. In eight planar on-surface assays, effect-directed tea profiles were revealed. Catechins and theaflavins turned out to be not only highly active, but also multi-potent compounds, able to act in a broad range of metabolic pathways. The flavan-3-ols acted as radical scavengers (DPPH∙ assay), antibacterials against Gram-positive Bacillus subtilis bacteria, and inhibitors of tyrosinase, α-glucosidase, β-glucosidase, and acetylcholinesterase. Further effects against Gram-negative Aliivibrio fischeri bacteria and β-glucuronidase were assigned to other components in the powdered tea extracts. According to their specifications, the activity responses of the powdered tea extracts were higher than in mere leaf extracts of green, white and black tea. The multi-imaging and effect-directed profiling was not only able to identify known functional food ingredients, but also to detect unknown bioactive compounds (including bioactive contaminants, residues or adulterations).

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Section: Resultsmentioning

confidence: 99%

Effect-Directed Profiling of Powdered Tea Extracts for Catechins, Theaflavins, Flavonols and Caffeine

et al. 2021

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“…HPTLC plates were prewashed for HRMS recording or in case of interfering α-/β-fronts. A set of plates was either fully immersed or developed with methanol-water, 4:1 (V/V), up to the upper plate edge in the Simultan Separating Chamber (biostep, Burkhardtsdorf, Germany), and dried in an oven at 110 °C for 20 min [ 60 , 61 ]. For storage in a desiccator, a clean counter glass plate was placed on top of the stacked plates, which were altogether wrapped in aluminum foil.…”

Section: Methodsmentioning

confidence: 99%

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

Morlock

Heil

2020

Anal Bioanal Chem

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Food products and botanicals on the global market need to be investigated in a more comprehensive way to detect effects, falsifications or adulterations. This is especially true for such ones containing Stevia leaves, Stevia extracts, or steviol glycosides. A multi-imaging profiling was developed exploiting hydrophilic interaction liquid chromatography (HILIC). A minimalistic sample preparation, different mixtures of acetonitrile and water/buffer on the silica gel phase as well as derivatization reagents and optional hyphenation with high-resolution mass spectrometry were exploited. The hydrophilic interaction high-performance thin-layer chromatography (HI-HPTLC) development took 10 min for 48 analyses. It was used to screen Stevia leaf extracts and 20 different food products. For the first time, the biological and biochemical profiling of Stevia leaf products by HI-HPTLC-UV/ Vis/FLD-assay pointed to 19 different bioactive compound bands found in the more natural multicomponent Stevia leaf extracts, whereas most of these activities were not existent for the steviol glycosides. The chemically isolated, purified, and EU-regulated steviol glycosides ease risk assessment and food product development. However, multipotent botanicals may have subtle impact on homeostasis via several metabolic pathways, providing benefits for the consumer's health. Analyzed side by side by means of the effect-directed profiling, their individual activity profiles were visualized as image and individual substances of importance were pointed out. Multi-imaging (comprehensive detection) plus non-targeted bioprofiling (focus on known and unknown bioactivity) allows for a fast detection of questionable product changes that occur along the global food chain and are particularly related to food safety.

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“…41 Useful detail information on the background and practical performance of HPTLC-MS has been reported. 46,47 Liquid Chromatography (LC) Offline and online separation of complex mixtures can be done by Liquid chromatography including Low pressure LC, high performance liquid chromatography (HPLC), ultra-high-performance liquid chromatography (UPLC), counter current chromatography (CCC) and other variants. 48 All these can be coupled with bio-assay and spectroscopy.…”

Section: Thin Layer Chromatography (Tlc) and High Performance Thin Lamentioning

confidence: 99%

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Elufioye¹

2017

TJNPR

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High‐Performance Thin‐Layer Chromatography–Mass Spectrometry for Analysis of Small Molecules

Cited by 7 publications

References 103 publications

Effect-Directed Profiling of Powdered Tea Extracts for Catechins, Theaflavins, Flavonols and Caffeine

Effect-Directed Profiling of Powdered Tea Extracts for Catechins, Theaflavins, Flavonols and Caffeine

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

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