High pH-induced flocculation–sedimentation and effect of supernatant reuse on growth rate and lipid productivity of Scenedesmus obliquus and Chlorella vulgaris

Castrillo, M.; Lucas-Salas, L.M.; Rodríguez-Gil, C.; Martinez, Daiana Gotardo

doi:10.1016/j.biortech.2012.10.114

Cited by 73 publications

(44 citation statements)

References 23 publications

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“…Final pH values were high and showed substantial DIC acquisition and photosynthetic capacity compared to other studies and species (Merrett et al 1996, Choo et al 2002, Ihnken et al 2011b). An in crease in pH also elevated growth in a Chlorella mass-culture (Castrillo et al 2013). Similar conclusions were made using marine algae in situ.…”

Section: Ph or Dic Effects?supporting

confidence: 73%

“…This clearly shows that alternative electron routes are employed by the cells when the pH of the external medium is perturbed. However, rapid alkalization causes cells to aggregate temporarily (Castrillo et al 2013), which can cause self-shading similar to the package-effect (Berner et al 1989). It is therefore possible that high τ PSII values are artifacts caused by cell aggregation after addition of the alkaline solution.…”

Section: Ph-dependent Acclimation Patternsmentioning

confidence: 99%

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Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

Ihnken¹,

Beardall²,

Kromkamp³

et al. 2014

Aquat. Biol.

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Chlorella spp. are robust chlorophyte microalgal species frequently used in mass culture. The pH optimum for growth is close to neutrality; at this pH, theoretically little energy is required to maintain homeostasis. In the present study, we grew Chlorella fusca cells in an open, outdoor, thin-layer cascade photobioreactor (TLC), under ambient photon flux at the theoretically preferred pH (7.2), and let the culture pass the exponential growth phase. Using pH drift experiments, we show that an alkalization to pH 9 supported photosynthesis in the TLC. The increased photosynthetic activity under alkaline conditions was a pH-dependent effect, and not a dissolved inorganic carbon (DIC) concentration-or light intensity-dependent effect. Re-acidification (in one step or in increments) lowered gross oxygen production and increased non-photochemical quenching in short-term experiments. Gross oxygen production and electron transport rates in PSII were uncoupled during the pH perturbation experiments. Electron transport rates were only marginally affected by pH, whereas oxygen production rates decreased with acidification. Alternative electron pathways, electron donation at the plastid terminal oxidase and state-transitions are discussed as a potential explanation. Because cell material from the TLC was not operating at maximal capacity, we propose that alkalization can support photosynthesis in challenged TLC systems.

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Section: Ph or Dic Effects?supporting

confidence: 73%

Section: Ph-dependent Acclimation Patternsmentioning

confidence: 99%

Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

Ihnken¹,

Beardall²,

Kromkamp³

et al. 2014

Aquat. Biol.

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“…The main microalgal harvesting processes include filtration, centrifugation, magnetic or electromagnetic separation, coagulation-flocculation, flotation and sedimentation or a combination of these processes. However, despite the abundance of microalgae recovery studies for microalgal biomass recovery, there is no consensus on the most appropriate methodology for this process 9,10 . The coagulation-flocculation process, followed by sedimentation, has been the most widely adopted method because it has been shown to be economically viable for large production volumes of biomass 11,12 .…”

Section: Introductionmentioning

confidence: 99%

Harvesting Microalgal Biomass grown in Anaerobic Sewage Treatment Effluent by the Coagulation-Flocculation Method: Effect of pH

Cassini

Francisco

Antunes

et al. 2017

Braz. arch. biol. technol.

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“…In 2013, an interesting investigation has been reported that concerns technologies for harvesting the biomass of microalgae by increasing the pH value in order to induce the flocculation-sedimentation of microalgae, such as Scenedesmus obliquus and Chlorella vulgaris [46]. The authors demonstrated that flocculation of microalgae can be induced by adding NaOH or Ca(OH) 2 to the culture.…”

Section: Cultivation Of Chlorella Vulgaris Ccala 896 In a Modified Bgmentioning

confidence: 99%

“…Although the highest pH value (about 10.7) reached in the flat photobioreactor used in the present study was not high enough to attain a total flocculation-sedimentation in the Chlorella vulgaris CCALA 896, this technology could certainly be improved. In order to cut down on the high costs of microalgae harvesting, which is one of the most expensive steps in microalgae production, the flocculation-sedimentation of Chlorella cells caused by a pH higher than 10.8 with the addition of Ca(OH) 2 [46] should be performed at the end of the Chlorella growth, when a pH value of 10.7 has already been attained. By doing this, the amount of Ca(OH) 2 required for the flocculation-sedimentation of cells could be reduced significantly.…”

Section: Cultivation Of Chlorella Vulgaris Ccala 896 In a Modified Bgmentioning

confidence: 99%

Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity

et al. 2014

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Abstract:The aim of this study was to induce lipid accumulation in Chlorella cells by creating stressful growth conditions. Chlorella vulgaris CCALA 896 was grown under various batch growth modes in basal and modified BG-11 and Kolkwitz culture broths, using a continuous light regimen of 150 µE/m 2 /s, at 30 °C . In order to perform the experiments, two indoor photobioreactor shapes were used: a cylindrical glass photobioreactor (C G PBR) with a working volume of 350 mL, and a flat glass photobioreactor (F G PBR) with a working volume of 550 mL. Stress-eliciting conditions, such as nitrogen and phosphorous starvation, were imposed in order to induce lipid accumulation. The results demonstrated that more than 56% of the lipids can be accumulated in Chlorella biomass grown under two-phase batch growth conditions. The highest biomass productivity of 0.30 g/L/d was obtained at the highest nominal dilution rate (0.167 day −1 ) during a semi-continuous regimen, using a modified Kolkwitz medium. During the pH-stress cycles, the amount of lipids did not increase significantly and a flocculation of Chlorella cells was noted. OPEN ACCESSEnergies 2014, 7 3841

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High pH-induced flocculation–sedimentation and effect of supernatant reuse on growth rate and lipid productivity of Scenedesmus obliquus and Chlorella vulgaris

Cited by 73 publications

References 23 publications

Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

Harvesting Microalgal Biomass grown in Anaerobic Sewage Treatment Effluent by the Coagulation-Flocculation Method: Effect of pH

Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity

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