High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

Kim, Ok-Hee; Booth, Carmen J.; Choi, Han Seok; Lee, Jinwook; Kang, Jiwon; Hur, June; Choi, Hyung Jin; Kim, Hyeonjin; Auh, Joong-Hyuck; Kim, Jung-Wan; Cha, Ji-Young; Lee, Young Jae; Lee, Cheol Soon; Choi, Cheolsoo; Yang, Jun-Young; Im, Seung‐Soon; Lee, Dong Hoon; Cho, Sun Wook; Kim, Young–Bum; Park, Kyong Soo; Park, Young Joo; Oh, Baek-Rock

doi:10.1101/816512

Cited by 2 publications

(2 citation statements)

References 54 publications

(70 reference statements)

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“…Slides were left to react with DAB substrate for 15 min, followed by mounting with a cover slip and dibutylphthalate polystyrene xylene (Sigma-aldrich, Missourti, MO, USA) mounting solution. Images were detected using a light microscope (Olympus, Tokyo, Japan) and quantification of the intensity of the brown color was performed using Image J software (NIH, Maryland, MD, USA) [ 45 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

The Reducing Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on High-Fat Diet-Induced Pyroptosis in Endothelial and Vascular Smooth Muscle Cells of Mice Aortas

Son

Park

et al. 2020

Marine Drugs

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In hyperlipidemia, pyroptosis in endothelial cells (ECs) induces atherosclerosis via the toll-like receptor 4 (TLR4) pathway. We evaluated the effects of Ecklonia cava extract (ECE) and pyrogallol-phloroglucinol-6,6-bieckol (PPB) on pyroptosis of ECs and vascular smooth muscle cells (VSMCs), which leads to attenuation of these cells and dysfunction of the aorta in high-fat-diet (HFD)-fed mice and in palmitate-treated ECs and VSMCs. The expression of TLR4 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which induce formation of NOD-LRR-and pyrin domain-containing protein 3 (NLRP3) inflammasomes, were increased by HFD and were decreased by ECE and PPB. The TLR4/NF-κB pathway was upregulated in palmitate-treated ECs and VSMCs and was decreased by ECE and PPB. The expressions of NLRP3/apoptosis-associated speck like protein containing a caspase recruitment domain, caspase-1, interleukin (IL)-1β, and IL-18 were increased by HFD and were decreased by ECE and PPB. Pyroptotic cells were increased by HFD and decreased by ECE and PPB. The expressions of the adhesion molecules, intercellular adhesion molecule and vascular cell adhesion molecule, and endothelin-1 were increased by HFD and were decreased by ECE and PPB. ECE and PPB decreased pyroptosis in the ECs and VSMCs, which was induced by HFD in the mouse aorta, and attenuated EC and VSMC dysfunction, an initiation factor of atherosclerosis.

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Section: Methodsmentioning

confidence: 99%

The Reducing Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on High-Fat Diet-Induced Pyroptosis in Endothelial and Vascular Smooth Muscle Cells of Mice Aortas

Son

Park

et al. 2020

Marine Drugs

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“…Genomic DNA was isolated from frozen stool samples using the QIAamp® Fast DNA Stool kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Bacterial DNA was amplified, targeting the V3-V4 regions of the 16S rRNA gene 47,48 . A subsequent amplification was performed to add multiplexing indices and the sequencing adapter.…”

Section: Microbiota Analysismentioning

confidence: 99%

Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis

Baek

Park

et al. 2021

Cell Death Dis

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Atopic dermatitis is a chronic skin inflammatory disease mediated by Th2-type immune responses. Although intestinal immune responses have been shown to play a critical role in the development or prevention of atopic dermatitis, the precise influence of intestinal immunity on atopic dermatitis is incompletely understood. We show here that orally tolerized mice are protected from experimental atopic dermatitis induced by sensitization and epicutaneous (EC) challenge to ovalbumin. Although the expression of Th2-type cytokines in the small intestine of orally tolerized and EC-challenged mice did not change significantly, these mice showed decreased inflammatory responses in the small intestine with restoration of microbial change elicited by the EC challenge. Interestingly, an increase in small intestinal eosinophils was observed with the EC challenge, which was also inhibited by oral tolerance. The role of small intestinal eosinophils and microbiota in the pathogenesis of experimental atopic dermatitis was further substantiated by decreased inflammatory mediators in the small intestine and attenuated Th2-type inflammation in the skin of eosinophil-deficient and microbiota-ablated mice with EC challenges. Based on these data, we propose that the bidirectional interaction between the skin and the intestine has a role in the pathogenesis of atopic dermatitis and that modulation of the intestinal microenvironments could be a therapeutic approach to atopic dermatitis.

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High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

Cited by 2 publications

References 54 publications

The Reducing Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on High-Fat Diet-Induced Pyroptosis in Endothelial and Vascular Smooth Muscle Cells of Mice Aortas

The Reducing Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on High-Fat Diet-Induced Pyroptosis in Endothelial and Vascular Smooth Muscle Cells of Mice Aortas

Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis

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