High-pressure cell for fluorescence fluctuation spectroscopy

Abstract

We introduce a high-pressure cell for fluorescence fluctuation spectroscopy. The pressure cell consists of a cylindrical, fused silica microcapillary and a modified pressure plug, which connects the cell to a pressure generating apparatus. The capillary serves both as the body and the optical window of the cell. The pressure cell is mounted onto the stage of an optical microscope. The small diameter of the capillary allows the use of high numerical aperture objectives, which are required for fluorescence fluctuation experiments. The pressure stability of the cell has been tested up to 4 kbar. We performed two-photon excitation experiments on simple dyes as a function of pressure. The diffusion coefficient was determined by fluorescence correlation spectroscopy. We also used the same cell to measure the pressure dependence of fluorescence lifetimes.

Pressure Response of Photoluminescence in Cesium Lead Iodide Perovskite Nanocrystals

“…The second apparatus is a hydraulic pressure setup, modified from a design by Tekmen et al, 39 with all components purchased from High Pressure Equipment Company (HIP, Erie, Pennsylvania). A hydraulic pressure generator (HIP, 25-5.75-100) capable of producing pressures as high as 700 MPa pressurized a set of stainless steel tubes filled with ethanol as the pressure-transmitting fluid.…”

Innovative insights in a plug flow microreactor foroperandoX-ray studies

“…As in their early work, Clausen et al (1991) did not expect to be able to estimate the maximum pressure a capillary could support. On the basis of the work of Tekmen & Mü ller (2004), a theoretical estimation of the maximum pressure supported by the capillaries was calculated. The quartz capillaries used have a 1 mm diameter with a 0.025 mm wall thickness.…”

Translational diffusion of probe molecules under high pressure: A study by fluorescence recovery after photobleaching technique

“…The technique that uses flexible fused silica microcapillaries to pressurize the sample has been developed previously by Müller and Gratton 24 and Tekmen et al 25 We describe here the setup used in our laboratory. 26 The solution containing fluorescent probe molecules fills a fused silica micro-capillary from Polymicro (USA).…”