High Pressure Liquid Chromato-Graphic Separation of Aryl-Naphthalide Lignan Lactones

Annapoorani, K.S.; Damodaran, C.; Sekharan, P. Chandra

doi:10.1080/01483918508067135

Cited by 8 publications

(4 citation statements)

References 5 publications

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“…[ 68 – 71 ] Other techniques that have been used for detection of toxin in blood and urine include high performance liquid chromatography (HPLC), spectrophotometry and thin-layer chromatography. [ 32 37 – 39 72 73 ]…”

Section: Introductionmentioning

confidence: 99%

Cleistanthus collinus poisoning

Chrispal

2012

J Emerg Trauma Shock

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Cleistanthus collinus, a toxic shrub, is used for deliberate self-harm in rural South India. MEDLINE (PUBMED) and Google were searched for published papers using the search/ MeSH terms “Cleistanthus collinus,” “Euphorbiaceae,” “Diphyllin,” “Cleistanthin A,” Cleistanthin B” and “Oduvanthalai.” Non-indexed journals and abstracts were searched by tracing citations in published papers. The toxic principles in the leaf include arylnaphthalene lignan lactones — Diphyllin and its glycoside derivatives Cleistanthin A and B. Toxin effect in animal models demonstrate neuromuscular blockade with muscle weakness, distal renal tubular acidosis (dRTA) and type 2 respiratory failure with conflicting evidence of cardiac involvement. Studies suggest a likely inhibition of thiol/thiol enzymes by the lignan-lactones, depletion of glutathione and ATPases in tissues. V-type H+ ATPase inhibition in the renal tubule has been demonstrated. Mortality occurs in up to 40% of C. collinus poisonings. Human toxicity results in renal tubular dysfunction, commonly dRTA, with resultant hypokalemia and normal anion gap metabolic acidosis. Aggressive management of these metabolic derangements is crucial. Acute respiratory distress syndrome (ARDS) is seen in severe cases. Cardiac rhythm abnormalities have been demonstrated in a number of clinical studies, though the role of temporary cardiac pacemakers in reducing mortality is uncertain. Consumption of decoctions of C. collinus leaves, hypokalemia, renal failure, severe metabolic acidosis, ARDS and cardiac arrhythmias occur in severe poisonings and predict mortality. Further study is essential to delineate mechanisms of organ injury and interventions, including antidotes, which will reduce mortality.

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Section: Introductionmentioning

confidence: 99%

Cleistanthus collinus poisoning

Chrispal

2012

J Emerg Trauma Shock

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“…This plant is commonly used as a suicidal and homicidal poison in Southeast Asian countries,[ 1 ] and cleistanthins A and B have been reported to be responsible for toxic effects in human beings. [ 2 3 ] Arylnaphthalide lignin glycosides are the major phytoconstituent of the genus Cleistanthus . They are also present in Phyllanthus taxodiifolius, Haplophyllum buxbaumii , and Cleistanthus patulus .…”

Section: Introductionmentioning

confidence: 99%

Biodistribution properties of cleistanthin A and cleistanthin B using magnetic resonance imaging in a normal and tumoric animal model

Parasuraman¹,

Raveendran²,

Ardestani³

et al. 2012

Phcog Mag

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Aim:To determine the biodistribution properties of cleistanthin A and cleistanthin B in rodents using magnetic resonance imaging (MRI).Materials and Methods:Cleistanthins A and B, constituents of Cleistanthus collinus Roxb., were labelled with gadolinium (Gd3+) directly and injected into normal and tumoric nude mice. The tissue signal intensity was measured using MRI to perform a noninvasive kinetic assay. Wistar rats were used for determination of the grayscale intensity to observe the distribution patterns of of cleistanthins A and B.Results:Cleistanthin A is kinetically more attractive to the gastrointestinal tract than is cleistanthin B, which gets accumulated in muscular tissues of mice in greater concentrations compared with cleistanthin A. Cleistanthin B but not cleistanthin A showed tumoric affinity and exhibited a tumor kinetic attraction in tumoric mice. In rats, cleistanthin A showed greater grayscale intensities in the brain, liver, and skeletal muscles in immediate post contrast MRI images, whereas the gadolinium tagged cleistanthin B showed higher grayscale intensities in the cardiac muscle and skeletal muscles in delayed post contrast MRI images.Conclusions:Cleistanthin A is more pharmacokinetically attractive to the gastrointestinal tract than cleistanthin B.

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“…[ 1 ] Cleistanthins A and B have been reported to be toxic, but scientific evidence for this claim is lacking. [ 2 ] Arylnaphthalide lignans are found in significant levels in plants of the genus Cleistanthus . Arylnaphthalide lignans include diphyllin, collinusin, cleistanthin, taiwanin, and other related compounds.…”

Section: Introductionmentioning

confidence: 99%

Diuretic Effects of Cleistanthin A and Cleistanthin B from the Leaves of Cleistanthus Collinus in Wistar Rats

Parasuraman¹,

Raveendran²

2012

Journal of Young Pharmacists

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To study the diuretic effects of cleistanthin A and cleistanthin B, phytoconstituents were isolated from the leaves of Cleistanthus collinus in Wistar rats. The in vivo diuretic effects of cleistanthins A and B were determined according to the Lipschitz test. Prior to the experiment, the animals were fasted for 5 h and placed individually in metabolic cages. Cleistanthins A and B (12.5, 25, and 50 mg/kg) and furosemide (5 mg/kg) were suspended in 0.5% w/v carboxymethyl cellulose and administered orally. The urine was collected up to 5 h after administration and subsequently up to 24 h after administration. The acidity and urine volume were measured immediately. The urinary sodium and potassium levels were determined using a flame photometer, and the chloride level was determined by argentometric titration. The diuretic index and diuretic activity were calculated mathematically. While cleistanthins A and B showed a diuretic index of more than one, the diuretic activity of these compounds was less than one, indicating inferior activity compared with furosemide. Both cleistanthin A and B produced a significant increase in the urine volume and alterations in urinary electrolyte levels. However, the effect of the compounds was not dose dependent. Cleistanthin A and cleistanthin B exert diuretic effects in male Wistar rats without affecting the urinary acidity.

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High Pressure Liquid Chromato-Graphic Separation of Aryl-Naphthalide Lignan Lactones

Cited by 8 publications

References 5 publications

Cleistanthus collinus poisoning

Cleistanthus collinus poisoning

Biodistribution properties of cleistanthin A and cleistanthin B using magnetic resonance imaging in a normal and tumoric animal model

Diuretic Effects of Cleistanthin A and Cleistanthin B from the Leaves of Cleistanthus Collinus in Wistar Rats

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