High Pressure Liquid Chromatographic Determination of Zearalenone in Chicken Tissues

Abstract

A method is reported for the extraction and analysis of zearalenone in chicken fat, heart muscle, and kidney tissue by using high pressure liquid chromatography (HPLC). Zearalenone is extracted with acetonitrile, cleaned up with hexane, and extracted further with ethyl acetate. Zearalenone is determined by HPLC using a reverse phase radial compression separation system, an ultraviolet absorbance detector, and a mobile phase of acetonitrile-water (60 + 40) (v/v). Recoveries of zearalenone added at levels from 50 to 200 ng/g are in the range 82.6-95.1%.

Indirect enzyme-linked immunosorbent assay for the mycotoxin zearalenone

“…In conclusion, we have described procedures for the production of zearalenone antibody in rabbits and for the use of this antibody in the competitive indirect ELISA for zearalenone. Indirect competitive ELISA would have significant advantages over existing methods which require extensive cleanup of the initial extract (9,13,(23)(24)(25)27). Gendloff et al (8) have shown that ELISA estimates of T-2 toxin in methanol extracts of corn correlate with gas-liquid chromatography.…”

“…The extensive biological effects of zearalenone require that its presence be routinely monitored in human foods and animal feeds. Current analytical methods for zearalenone such as thin-layer chromatography (TLC) (8,9,23,24), gas-liquid chromatography (23,25), and high-pressure liquid chromatography (13,23,27) necessitate time-consuming extraction and sample clean-up and are thus unsuitable for the routine screening of large sample numbers. Recently, a radioimmunoassay (RIA) was developed as an alternative for detecting zearalenone in clinical samples (26).…”

Hybridoma cell line production of a specific monoclonal antibody to the mycotoxins zearalenone and .alpha.-zearalenol

“…Current methods for analysis of zearalenone in foods and feeds include thin-layer chromatography (TLC) (AOAC, 1984;Gimeno, 1983; Scott et al, 1978;Swanson et al, 1984), gas-liquid chromatography (GLC) (Scott et al, 1978; Thouvenot and Morfin, 1979), and liquid chromatography (Bennett et al, 1985, James et al, 1982; Scott et al, 1978;Turner et al, 1983). These methods require extensive extraction and sample cleanup and therefore are not readily applicable to routine screening of large numbers of samples for zearalenone.…”

Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water

“…Analytical methods for detection include colorimetric and fluorescence-based strategies (e.g., enzyme-linked immunosorbent assay, ELISA), chromatographic methods, and enzyme-linked oligonucleotide assays [ 39 ]. Traditional chromatographic separation, e.g., high-performance liquid chromatography (HPLC) [ 40 , 41 , 42 ], thin layer chromatography (TLC) [ 43 , 44 , 45 ], and liquid or gas chromatography coupled with mass spectroscopy (LC or GC MS) [ 46 , 47 , 48 , 49 ] have low limit of detection (LOD) and limit of quantification (LOQ) values but, usually, due to the complex sample preparation they are time consuming technologies requiring special instrumentation. Immunoanalytical techniques are cost-effective and suitable for rapid monitoring with detecting multiple samples at the same time [ 50 , 51 , 52 , 53 ].…”