High Prevalence of Antibiotic Resistance and Integrons in<i>Escherichia coli</i>Isolated from Urban River Water, India

Kaushik, Megha; Khare, Neha; Kumar, Sanjay; Gulati, Pooja

doi:10.1089/mdr.2018.0194

Cited by 28 publications

(22 citation statements)

References 46 publications

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“…The dfrA17-aadA5 integron (In54) cassette was first characterised in 2000 after its discovery in an E. coli isolated from a urinary tract infection (UTI) patient in Australia ( White et al, 2000 ). Since then, dfrA17-aadA5 has become one of the most prevalent cassette arrangements worldwide in E. coli ( Yu et al, 2004 ; Xia et al, 2016 ; Kaushik et al, 2019 ) and in other Enterobacteriaceae including Salmonella species ( Meng et al, 2017 ), and is thought to have driven trimethoprim resistance in ExPEC ST69 strains (also known as clonal group A) ( Solberg et al, 2006 ). While the dfrA17-aadA5 cassette has been previously identified in integrons present in clinical ETEC strains ( Kartsev et al, 2015 ), a BLASTn search demonstrated that the entire IS 26 flanked CRR containing the integron found in ST182 airplane isolates has not been observed in ETEC previously, but in Klebsiella, Salmonella and ExPEC-associated STs, most commonly in ST131.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Lineage of Enterotoxigenic Escherichia coli ST182 With Serotype O169:H41 in Airline Waste

et al. 2021

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Enterotoxigenic Escherichia coli (ETEC) is the primary aetiologic agent of traveller’s diarrhoea and a significant cause of diarrhoeal disease and death in developing countries. ETEC O169:H41 strains are known to cause both traveller’s diarrhoea and foodborne outbreaks in developed countries and are cause for concern. Here, whole-genome sequencing (WGS) was used to assemble 46 O169:H41 (ST182) E. coli draft genomes derived from two airplane waste samples sourced from a German international airport. The ST182 genomes were compared with all 84 publicly available, geographically diverse ST182 genomes to construct a core genome-based phylogenetic tree. ST182 isolates were all phylogroup E, the majority serotype O169:H41 (n = 121, 93%) and formed five major clades. The airplane waste isolates differed by an average of 15 core SNPs (range 0–45) but their accessory genome content was diverse. While uncommon in other ST182 genomes, all airplane-derived ST182 isolates carried: (i) extended-spectrum β-lactamase gene blaCTX–M–15 notably lacking the typical adjacent ISEcp1; (ii) qnrS1 and the S83L mutation in gyrA, both conferring resistance to fluoroquinolones; and (iii) a class 1 integron structure (IS26-intI1Δ648-dfrA17-aadA5-qacEΔ1-sul1-ORF-srpC-padR-IS6100-mphR-mrx-mphA-IS26) identified previously in major extraintestinal pathogenic E. coli STs but not in ETEC. ST182 isolates carried ETEC-specific virulence factors STp + CS6. Adhesin/invasin tia was identified in 89% of aircraft ST182 isolates (vs 23%) and was located on a putative genomic island within a hotspot region for various insertions including PAI I536 and plasmid-associated transposons. The most common plasmid replicons in this collection were IncFII (100%; F2:A-:B-) and IncB/O/K/Z (89%). Our data suggest that potentially through travel, E. coli ST182 are evolving a multidrug-resistant profile through the acquisition of class 1 integrons and different plasmids.

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Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Lineage of Enterotoxigenic Escherichia coli ST182 With Serotype O169:H41 in Airline Waste

et al. 2021

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“…This also implied that Tn 7 -like transposons were likely to occur simultaneously with other mobile elements. High resistance to streptomycin (87.8%) and trimethoprim/sulfamethoxazole (74.0%) can be attributed to the intI2 -associated resistance gene cassette ( aadA1 , sat2 , and dfrA1 ) carried by Tn 7 -like transposons ( Tietze and Brevet, 1991 ; Kaushik et al, 2019 ). The gene cassettes of intI2 contained the aminoglycoside adenyltransferase ( aadA1 and aadA2 ), dihydrofolate reductase ( dfrA1 ), and streptothricin acetyltransferase ( sat2 ) encoding genes, which are responsible for streptomycin-spectinomycin, trimethoprim, and streptothricin resistance, respectively ( Zhang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Detection of Tn7-Like Transposons and Antibiotic Resistance in Enterobacterales From Animals Used for Food Production With Identification of Three Novel Transposons Tn6813, Tn6814, and Tn6765

Cui

et al. 2020

Front. Microbiol.

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Enterobacterales are widely distributed in the gastro-intestinal system of animals and may cause opportunistic infections. Worse still, multidrug-resistant Enterobacterales also poses a serious threat to public health. Tn7-like transposons have been found in several species of the Enterobacterales order and play an important role in dissemination of antibiotic resistance. This study aimed to investigate the distribution and genetic characterization of Tn7-like transposons in Enterobacterales isolates from food animals and their association with antibiotic resistance. Enterobacterales isolated from the samples were identified and classified according to the 16S rDNA sequence. Tn7-like transposons and associated integrons were detected by polymerase chain reaction (PCR) and sequencing. The antibiotic resistance of each Tn7-like transposon positive isolate was detected according to the Kirby-Bauer disk diffusion method. Then, six representative strains were selected to study the genetic environment by whole-genome sequencing (WGS). In total, we isolated 377 Tn7-like transposons positive strains of Enterobacterales. Class 2 integrons were detected in 99.5% of the isolates, and there were high frequency mutation sites especially in base 535, a stop mutation. Many isolates (54.9%) were multidrug-resistant and observed high resistance rates to trimethoprim/sulfamethoxazole and streptomycin. Among these strains, we found three new types of Tn7-like transposons, named Tn6813, Tn6814, and Tn6765. This is the first comprehensive survey that shows Tn7-like transposons in Enterobacterales from animals used for food production in different regions of China. This study also provides an insight into the horizontal transfer of resistance genes associated with Tn7-like transposons.

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“…Tn7-like isolates are particularly resistant to Trimethoprim/Sulfamethoxazole and Streptomycin because of the intI2-associated resistance gene cassette (aadA1,sat2 and dfrA1) carried by Tn7-like (43,44). The gene cassettes of intI2 contained the aminoglycoside adenyltransferase (aadA1 and aadA2), dihydrofolate reductase (dfrA1), and streptothricin acetyltransferase (sat2) encoding genes, which are responsible for streptomycin-spectinomycin, trimethoprim, and streptothricin resistance, respectively area (45).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Tn7-like transposons and its related antibiotic resistance among Enterobacteriaceae from livestock and poultry in China

He¹,

Li²,

Cui³

et al. 2020

Preprint

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Background: This study was aimed to investigate the prevalence and structure of Tn7-like in Enterobacteriaceae from livestock and poultry as well as their possible role as reservoir of antibiotic resistance genes (ARGs). Methods:Polymerase chain reaction (PCR) and DNA sequencing analyses were used for the characterization of Tn7-like, associated integrons and ARGs. The antimicrobial resistance profile of the isolates was examined by using disc diffusion test.Results: Three hundred and seventy-eight Tn7-like-positive strains of Enterobacteriaceae were isolated, and included E. coli (128), Proteus(150), K. pneumonia(17), Salmonella(13), M. morganii (21) and A. baumannii(1), wherein high resistance was observed for Trimethoprim/Sulfamethoxazole and Streptomycin, and fifty percent of the strains were multidrug-resistant. Integrons class 2 were detected in all of the isolates and there are high frequency mutation sites especially in 535, a stop mutation. Variable region of class 2 integrons carried same gene cassettes, namely aadA1-sat2-dfrA1.From the 378 isolated strains, we found a new type of Tn7-like on a plasmid, named Tn6765. Conclusions:These findings proved that the Tn7-like can contribute to the horizontal transmission of antibiotic resistant genes in livestock and poultry. As potential vessels for antibiotic resistance genes (ARGs), Tn7-like could not be ignored due to their efficient transfer ability in environments.

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High Prevalence of Antibiotic Resistance and Integrons inEscherichia coliIsolated from Urban River Water, India

Cited by 28 publications

References 46 publications

Multidrug-Resistant Lineage of Enterotoxigenic Escherichia coli ST182 With Serotype O169:H41 in Airline Waste

Multidrug-Resistant Lineage of Enterotoxigenic Escherichia coli ST182 With Serotype O169:H41 in Airline Waste

Detection of Tn7-Like Transposons and Antibiotic Resistance in Enterobacterales From Animals Used for Food Production With Identification of Three Novel Transposons Tn6813, Tn6814, and Tn6765

Characterization of Tn7-like transposons and its related antibiotic resistance among Enterobacteriaceae from livestock and poultry in China

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