High prevalence of B2-ST131 clonal group among extended-spectrum β-lactamase-producing Escherichia coli isolated from bloodstream infections in Quito, Ecuador

Zurita, Jeannete; Solís, María Belén; Ortega-Paredes, David; Barba, Pedro; Miño, Ariane Paz y; Sevillano, Gabriela

doi:10.1016/j.jgar.2019.04.019

Cited by 10 publications

(9 citation statements)

References 35 publications

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“…E. coli ‐ST410 has been identified as a new pandemic clone (Schaufler et al ), and it was previously reported in vegetable samples (Ortega‐Paredes et al ) and clinical samples (Zurita et al ) in Ecuador. E. coli ‐ST410 has been isolated from animal (Zogg et al ) and environmental samples, suggesting that this clone plays a relevant role in the human–animal–environment interface proposed by the ‘One Health’ concept (Wielinga and Schlundt ).…”

Section: Resultsmentioning

confidence: 89%

“…Ready‐to‐eat salads carried MDR E. coli with genes coding for resistance to antibiotics that are considered critically important for human medicine as fluoroquinolones and third‐generation cephalosporins (WHO ). The dissemination of the bla CTX‐M‐15 gene is the leading cause of β‐lactam antibiotic resistance in the E. coli isolates most frequently reported in clinical samples in Ecuador (Zurita et al ). However, in this study, the most commonly encountered resistance genes were bla CTX‐M‐55 and bla CTX‐M‐65 , which have also been previously reported in Ecuador (Zurita et al ; Ortega‐Paredes et al ) in broiler production samples (Vinueza et al ), canine faeces (Ortega‐Paredes et al ) and urban rivers (Ortega‐Paredes et al ).…”

Section: Resultsmentioning

confidence: 99%

“…Escherichia coli ‐ST131 has been isolated from humans, animals and environmental samples in different countries, and due to its extraordinary capacity for disseminating and propagating ESBL resistance genes, it represents a potential threat to human health (Rogers et al ). We identified one ST131‐B2 isolate (5%) harbouring bla CTX‐M‐15 , described as an epidemic clone with broad dissemination (Nicolas‐Chanoine et al ) that is found mainly in clinical samples in Ecuador (Zurita et al ). The presence of E. coli‐ ST131 in a single sample is probably due to the contamination during the manipulation of the food sample.…”

Section: Resultsmentioning

confidence: 99%

“…Escherichia coli is the main ESBL‐producing bacterium identified in food products, human and environmental samples worldwide (Rawat and Nair ; Ojer‐usoz et al ). In Ecuador, ESBL‐producing E. coli strains have been characterized in clinical samples, raw vegetables, dog faeces and broiler chickens (Gestal et al ; Ortega‐Paredes et al ; Chiluisa‐Guacho et al ; Soria Segarra et al ; Hedman et al ; Vinueza et al ; Zurita et al ). However, there is no data about the contamination of street food with ESBL‐producing E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

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Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Zurita

Yánez

Sevillano³

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: This study identified ESBL-producing Escherichia coli epidemic clones: ST131, ST410 and ST744 in ready-to-eat street food samples. Street food is a possible way to spread harm multidrug-resistant (MDR) E. coli strains in the community. Studies to identify the contamination sources of this kind of food are needed to tackle MDR E. coli dissemination. AbstractReady-to-eat food contamination with ESBL-producing Escherichia coli is a growing health concern. Some of these strains also are epidemic clones and can cause community-associated infections that are difficult to treat. In this study, the occurrence of ESBL-producing E. coli contaminated ready-to-eat street food in Quito, Ecuador was evaluated. In total, 150 samples were collected randomly in the most crowded sites of the city. In all, 34 samples (34/150; 22Á6%) were positive for total thermotolerant (44Á5°C) coliforms resistant to cefotaxime. MALDI-TOF analysis identified that the E. coli was found in 20 food samples (20/34; 59%). ESBL gene bla CTX-M-55 was identified in nine isolates, bla CTX-M-15 in six isolates, bla CTX-M-14 in two isolates, and one isolate each harboured bla CTX-M-24 , bla CTX-M-65 , bla CTX-M-55 and bla CTX-M-8 . Phylogenetic groups like A and B1 were the most common, followed by groups D and B2. MLST analysis identified 12 different sequence types (STs), the most common was ST162. Recognized epidemic clonal groups ST410, ST131 and ST744 were encountered. Ready-to-eat street food is a potential way of spreading ESBL-producing E. coli epidemic clones in Quito, Ecuador.Letters in Applied Microbiology ISSN 0266-8254

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Zurita

Yánez

Sevillano³

et al. 2020

Lett Appl Microbiol

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“…On the other hand, CTX-M-15 was observed in ST131 E. coli strains (Alsharapy et al, 2018). As it becomes evident, ST131 is highly associated with ESBL production in the Middle Eastern region as well as other countries across the world: Israel (Karfunkel et al, 2013), KSA (Alghoribi et al, 2015; Alyamani et al, 2017; Yasir et al, 2018), Iran (Moghanni et al, 2018), Bulgaria (Markovska et al, 2012), Ecuador (Zurita et al, 2019), and Spain (Merino et al, 2016).…”

Section: Distribution Of Multi-drug Resistant Organisms In Humansmentioning

confidence: 99%

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

et al. 2019

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In the last decade, extended-spectrum cephalosporin and carbapenem resistant Gram-negative bacilli (GNB) have been extensively reported in the literature as being disseminated in humans but also in animals and the environment. These resistant organisms often cause treatment challenges due to their wide spectrum of antibiotic resistance. With the emergence of colistin resistance in animals and its subsequent detection in humans, the situation has worsened. Several studies reported the transmission of resistant organisms from animals to humans. Studies from the middle east highlight the spread of resistant organisms in hospitals and to a lesser extent in livestock and the environment. In view of the recent socio-economical conflicts that these countries are facing in addition to the constant population mobilization; we attempt in this review to highlight the gaps of the prevalence of resistance, antibiotic consumption reports, infection control measures and other risk factors contributing in particular to the spread of resistance in these countries. In hospitals, carbapenemases producers appear to be dominant. In contrast, extended spectrum beta lactamases (ESBL) and colistin resistance are becoming a serious problem in animals. This is mainly due to the continuous use of colistin in veterinary medicine even though it is now abandoned in the human sphere. In the environment, despite the small number of reports, ESBL and carbapenemases producers were both detected. This highlights the importance of the latter as a bridge between humans and animals in the transmission chain. In this review, we note that in the majority of the Middle Eastern area, little is known about the level of antibiotic consumption especially in the community and animal farms. Furthermore, some countries are currently facing issues with immigrants, poverty and poor living conditions which has been imposed by the civil war crisis. This all greatly facilitates the dissemination of resistance in all environments. In the one health concept, this work re-emphasizes the need to have global intervention measures to avoid dissemination of antibiotic resistance in humans, animals and the environment in Middle Eastern countries.

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WHO Critical Priority Escherichia coli in Latin America: A One Health Challenge for a Post-Pandemic World

Lincopán

Fuentes‐Castillo

Espinoza-Muñoz³

et al. 2023

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High prevalence of B2-ST131 clonal group among extended-spectrum β-lactamase-producing Escherichia coli isolated from bloodstream infections in Quito, Ecuador

Cited by 10 publications

References 35 publications

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

WHO Critical Priority Escherichia coli in Latin America: A One Health Challenge for a Post-Pandemic World

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