High progesterone concentrations induce acrosome reaction with a low cytotoxic effect

Parinaud, Jean; Labal, B.; Vieitez, G.

doi:10.1016/s0015-0282(16)55270-4

Cited by 49 publications

(27 citation statements)

References 21 publications

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“…In contrast, Foresta et al (1992) and Uhler et al (1992) did not find a stimulating effect of the AR of sperm incubated for 8-24 h with progesterone during capacitation. Parinaud et al (1992) found an increased rate of AR after coincubating cells with progesterone during capacitation, but with the use of unphysiologically high concentrations of the steroid (314.5 p,g/ml). An increase in [Ca2+Ii is found in uncapacitated as well as in capacitated spermatozoa.…”

Section: Direct Initiation Of the Ar By Progesterone Cbg Or Cbg-promentioning

confidence: 95%

Further indications of the multicomponent nature of the acrosome reaction‐inducing substance of human follicular fluid

Fehl

Miska

Henkel

1995

Molecular Reproduction Devel

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Human follicular fluid (hFF), which has been treated with either unspecific proteases or dextran-coated charcoal (DCC) to remove proteins and/or steroids, cannot successfully induce the acrosome reaction (AR). After the removal of steroids, AR-inducing activity can be restored to hFF by supplementation with exogenous progesterone, but only in the presence of intact protein. Gel filtration experiments with 3H-progesterone-labelled hFF showed elution of the radioactive signal in the high molecular weight range, corresponding to bound progesterone. AR-inducing activity was seen in exactly the same fraction. Based on these results, the acrosome reaction-inducing substance (ARIS) appears to be a complex of progesterone and a progesterone-binding protein, which was shown to be identical with the plasma protein corticosteroid-binding globulin (CBG) by immunological techniques. AR induction was only observed in the presence of both CBG and progesterone, suggesting a combined effect of the two components.

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Section: Direct Initiation Of the Ar By Progesterone Cbg Or Cbg-promentioning

confidence: 95%

Further indications of the multicomponent nature of the acrosome reaction‐inducing substance of human follicular fluid

Fehl

Miska

Henkel

1995

Molecular Reproduction Devel

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“…First, we attempted to maximize the response of cells to progesterone challenge. Second, there is no indication that a high concentration of progesterone (up to 1 mM) affects cell viability [48]. Third, spermatozoa may, after all, be acutely exposed to a high concentration of progesterone on the cell surface [49].…”

Section: Resultsmentioning

confidence: 99%

Bicarbonate/CO₂ induces rapid activation of phospholipase A₂ and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

Roldán

Vázquez

1996

FEBS Letters

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We tested whether capacitation, a phenomenon that renders spermatozoa capable of undergoing acrosomal exocytosis, may be completed rapidly after a short exposure to bicarbonatelCO2. We found that, in the presence of Ca 2+, a 10-min exposure of boar spermatozoa to bicarbonate led to a partial activation of phospholipase A2, primed spermatozoa for a major subsequent activation of this enzyme upon stimulation with progesterone and furthermore rendered spermatozoa capable of undergoing exocytosis in response to this steroid. These results suggest that capacitation may be completed in a relatively short period of time and open up new possibilities for unravelling molecular mechanisms underlying this process.

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“…Progesterone and its derivatives have been reported to act as agonists against GABAA receptor in the neural tissues [17,18]. It was recently found that mammalian sperm AR could be promoted by progesterone [9, 10], and several authors insisted on the possibility that the action of progesterone acted as GABAA-like receptor [11,12]. We, therefore, investigated the interaction of progesterone and GABAA receptor on calcium mobilization during human sperm AR.…”

Section: Discussionmentioning

confidence: 99%

“…Although the agent had been so far understood to interact with intracellular receptor, several authors reported that it might act as an agonist against cell surface r-aminobutyric acid with type A (GABAA) receptor [11,12]. Its mode of action is, however, still a controversial issue.…”

mentioning

confidence: 99%

Influence of Progesterone and Gaba a Receptor on Calcium Mobilization During Human Sperm Acrosome Reaction

Kuroda

Kaneko

Yoshimura

et al. 1999

Archives of Andrology

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High progesterone concentrations induce acrosome reaction with a low cytotoxic effect

Cited by 49 publications

References 21 publications

Further indications of the multicomponent nature of the acrosome reaction‐inducing substance of human follicular fluid

Further indications of the multicomponent nature of the acrosome reaction‐inducing substance of human follicular fluid

Bicarbonate/CO₂ induces rapid activation of phospholipase A₂ and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

Influence of Progesterone and Gaba a Receptor on Calcium Mobilization During Human Sperm Acrosome Reaction

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High progesterone concentrations induce acrosome reaction with a low cytotoxic effect

Cited by 49 publications

References 21 publications

Further indications of the multicomponent nature of the acrosome reaction‐inducing substance of human follicular fluid

Further indications of the multicomponent nature of the acrosome reaction‐inducing substance of human follicular fluid

Bicarbonate/CO2 induces rapid activation of phospholipase A2 and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

Influence of Progesterone and Gaba a Receptor on Calcium Mobilization During Human Sperm Acrosome Reaction

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Bicarbonate/CO₂ induces rapid activation of phospholipase A₂ and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone