High‐rate ethanol production at low pH using the anaerobic granular sludge process

Tamis, Jelmer; Joosse, Bart; Leeuw, Kasper D. de; Kleerebezem, Robbert

doi:10.1002/bit.27708

Cited by 9 publications

(2 citation statements)

References 27 publications

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“…As shown in Figure 8, at the genus level, Lactobacillus was detected in the fermentation sludge, representing 54.06% of the total, and many studies have reported it as a dominant genus in anaerobic activated sludge and acid-producing fermentation processes [27][28][29]. Therefore, the presence of the Lactobacillus genus is favorable for the acid production in sludge fermentation, resulting in an acid production rate of over 88.4%.…”

Section: Analysis Of Functional Microbial Communitymentioning

confidence: 99%

Exploring 2,4,6-Trichlorophenol Degradation Characteristics and Functional Metabolic Gene Abundance Using Sludge Fermentation Broth as the Carbon Source

Wang,

2023

Water

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The use of sludge fermentation broth (FB) as a co-metabolic carbon source for treating 2,4,6-trichlorophenol (2,4,6-TCP) wastewater is a novel strategy. The key to the feasibility of this strategy is whether the FB can promote the growth of functional microorganisms that are capable of degrading 2,4,6-TCP. This study focused on long-term acclimatized sludge and investigated the impact of key operating parameters such as the sludge FB concentration and the influent concentration of 2,4,6-TCP on the removal efficiency of chlorophenol. The research findings revealed that when the influent concentration of sludge FB exceeded 300 mg COD/L, it significantly inhibited the degradation of 2,4,6-TCP. Simulation experiments using individual VFA components as influent carbon sources showed that excessive propionic acid addition can inhibit the degradation of 2,4,6-TCP, indicating the need to control the concentration of propionic acid in the fermentation conditions. Metagenomic analysis further showed that sludge FB can promote the enrichment of microbial chlorophenol degradation genes, including PcpA, pcaF, pcaI, Mal-r, chqB, and fadA. The abundances of these six chlorophenol degradation genes were as follows: 1152 hits (PcpA), 112 hits (pcaF), 10,144 hits (pcaI), 12,552 hits (Mal-r), 8022 hits (chqB), and 20,122 hits (fadA). Compared with other types of carbon sources, sludge FB demonstrates distinct advantages in terms of leading to the highest chlorophenol degradation concentration and the abundance of functional microbial communities. This study has successfully demonstrated the feasibility of using sludge FB as a co-metabolic carbon source for the degradation of 2,4,6-TCP.

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Section: Analysis Of Functional Microbial Communitymentioning

confidence: 99%

Exploring 2,4,6-Trichlorophenol Degradation Characteristics and Functional Metabolic Gene Abundance Using Sludge Fermentation Broth as the Carbon Source

Wang,

2023

Water

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“…Chemical oxygen demand (COD) is one of the important analytical parameters for counting pure or mixed organic matters in aqueous solution (ASTM D1252-06, 2020ISO 15705, 2002;IUPAC, 2019;Baker et al, 1999). It is widely used in wastewater, aerobic digestion, and anaerobic digestion (Contreras et al, 2002;Brouckaert et al, 2021;Ahnert et al, 2021;Brouckaert et al, 2022;Angelidaki & Sanders, 2004;Tamis et al, 2021). The quantity of COD of organic matter is relevant to the quantitative amount of molecular oxygen (O 2 ).…”

Section: Introductionmentioning

confidence: 99%

Using Mean Oxidation Number of Organic Carbons to Count Theoretical Chemical Oxygen Demand

Yuen,

Lau

2024

IJC

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Chemical oxygen demand and mean oxidation number of organic carbons are two important concepts in redox chemistry. The former is used for counting pure or mixed organic matters in aqueous solution. The latter is a redox metric for water treatment, organic combustion, and anaerobic digestion. Currently the calculation of theoretical chemical oxygen demand of neutral organic matter is based on the number of moles of molecular oxygen (O2). However, the calculation of theoretical chemical oxygen demand of ionic organic matter has seldom been studied. The purpose of this article is to develop a simple mathematical equation for doing so by using mean oxidation number of organic carbons. To develop the equation, relationships among chemical oxygen demand, mean oxidation number of organic carbons, number of organic carbons, and formula mass of organic matter are identified. The mathematical equations for chemical oxygen demand, total organic carbon, and the ratio of chemical oxygen demand to total organic carbon are also established for any molecule(s).

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High‐rate ethanol production at low pH using the anaerobic granular sludge process

Tamis

Joosse

Leeuw

et al. 2021

Biotech & Bioengineering

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In this study, we investigated the operational performance and product spectrum of glucose‐fermenting anaerobic granular sludge reactor at pH 4. A selective environment for the growth of granules was implemented by the introduction of a 2 min settling phase, a hydraulic retention time of 6 h and a solid retention time of 12 ± 3 days. The fermentation products were ethanol, lactate, and volatile fatty acids (VFA) with yields of 0.55 ± 0.03, 0.15 ± 0.02, and 0.20 ± 0.04 gram chemical oxygen demand (gCOD)/gCOD glucose, respectively. The obtained product spectrum was remarkably different from the VFA‐dominated product spectrum reported in a previous study when the same system was operated at higher pH (4.5–5.5). The shift in product spectrum coincided with a shift in the microbial community structure with the dominance of eukaryotic Candida tropicalis, Pichia jaroonii, and prokaryotic Lactobacillus species instead of the Clostridia species obtained at higher pH‐values. The control of the microbiomes and the associated product spectra provides bioprocess engineers with the option to tailor a suitable precursor compound mixture for subsequent chain elongation fermentation or PHA biopolymer production.

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High‐rate ethanol production at low pH using the anaerobic granular sludge process

Cited by 9 publications

References 27 publications

Exploring 2,4,6-Trichlorophenol Degradation Characteristics and Functional Metabolic Gene Abundance Using Sludge Fermentation Broth as the Carbon Source

Exploring 2,4,6-Trichlorophenol Degradation Characteristics and Functional Metabolic Gene Abundance Using Sludge Fermentation Broth as the Carbon Source

Using Mean Oxidation Number of Organic Carbons to Count Theoretical Chemical Oxygen Demand

High‐rate ethanol production at low pH using the anaerobic granular sludge process

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