of these scenarios. [1] Migration is influenced by chemotactic, topographical, and mechanotransductive cues from the extracellular matrix. [2] Moreover, cell migration in the context of cancer metastasis is a complex and important factor for understanding tumor progression. [3] The most aggressive form of a primary brain tumor is glioblastoma multiforme (GBM) which are highly invasive heterogenous tumors with a very low survival rate. [4] Surgical resection and chemo-or radiotherapy is commonly used for patient treatment, however, tumor recurrence is very frequent. Importantly, GBM cells invade and migrate along white matter tracts and brain blood vessels which promote tumor dissemination. [5] Hence, it is critical to understand the basic process of tumor migration and progression in order to develop new therapeutic drugs and treatment regimens. While therapeutic approaches that minimize GBM migration are logical, another approach focuses on guiding these cells away from the tumor into biomaterial reservoirs with the goal to reduce tumor size. [6] This diversional approach is based on the placement of a tube filled with a matrix and oriented substrate that provides topographical guidance cues at the tumor site. This results in the attraction and guidance of migration of GBM cells into the tube, effectively reducing overall tumor size. Therefore, in line with this study and the fact that GBM cells have an affinity for white brain matter and blood vessels, it is important to develop new 3D in vitro cell culture models to determine the optimal matrix composition that drives GBM migration. Novel research methods and tools provide an opportunity to study cell migration during cancer metastasis [7] where loss of cell adhesion from the primary tumor along with increased cell motility and invasion occurs. There is evidence from 3D microfluidic devices and microchips that matrix stiffness influences the migratory and invasive capabilities of tumor cells through the structure characteristics. [8] There have been significant advances to understand the process of cell migration using in vitro models, which are cost effective and easier to use compared to in vivo studies. With existing in vitro assays, cell migration conditions are welldefined with many based on the traditional 2D cell culture methods. [9] While simple to use, they are challenged to recapitulate the 3D in vivo microenvironment.