High-Resolution Gas-Chromatographic Analysis of the Volatile Constituents of Body Fluids, with Use of Glass Capillary Columns

Abstract

Trace volatile constituents of human urine, serum, and cerebrospinal fluid were concentrated on a porous polymer precolumn, and resolved with high-efficiency glass capillary columns. Thin-film glass columns facilitate effective resolution of underivatized compounds within the volatility range up to docosane in relatively short analysis times. Glass capillary columns coated with stationary phases of different polarity were used. Recorded volatile metabolic profiles of human urine are altered after the sample is treated with β-glucuronidase and sulphatase.

High-resolution gas chromatographic profiles of volatile organic compounds produced by microorganisms at refrigerated temperatures

“…---+ X --* h + - Capillary-column gas chromatograms of the volatiles produced by a Moraxella oxidative culture (A) and a P. putida culture (B). organic compounds from the headspace of a number of samples including body fluids (14,17), drinking water (5, 6), tobacco leaf aroma (13), and air (3, 15). Tenax GC has an extremely high capacity for the retention of organic compounds and little retention for water.…”

“…Cultures of Pseudomonas putida, Pseudomonas fluorescens, and a Moraxella oxidative genus, previously isolated from spoiled, uncooked chicken (7), were grown separately on prepoured TSY agar plates enclosed in oxygen-permeable, medium-density polyethylene bags to aid in moisture control. The cultures were allowed to incubate for 14 days at 2 to 40C. The TSY agar medium was composed of 4% Trypticase soy agar (BBL) supplemented with 0.5% yeast extract (Difco).…”

Application and characteristics of polymer adsorption method used to analyze flavor volatiles from peanuts

“…In recent years, adsorption polymers have been used for collection, concentration, and subsequent GC analyses in a wide variety of applications. Novotny et al (1974a) used a porous polymer adsorption precolumn for high-resolution GC analysis of the volatile constituents of body fluids. Murray (1977) described a technique for concentrating headspace, airborne, and aqueous volatiles on a porous polymer precolumn, and Zeldes and Horton (1978) used Tenax GC adsorbent polymer to trap volatiles in cigarette smoke.…”

Scent and the single male: ring‐tailed lemurs produce honest signals

“…Gas chromatography and mass spectrometry revealed many of the volatile compounds, semiochemicals, that composed various types of scent marks in many species (Novotny et al . 1974a,b; Albone 1984). Currently, because of advances in molecular genetics, interest in the study of scent has been reinvigorated, in particular, in the relationship of genes and scent production.…”