High-resolution gas chromatographic profiles of volatile organic compounds produced by microorganisms at refrigerated temperatures

Abstract

Three different strains of bacteria isolated from spoiled, uncooked chicken were grown in pure culture on Trypticase soy agar supplemented with yeast extract. The volatile organic compounds produced by each culture were concentrated on a porous polymer precolumn and analyzed by high-resolution gas chromatographic mass spectrometry. Twenty different compounds were identified. Both qualitative and quantitative differences in the chromatographic profiles from each culture were found.

Headspace analysis of volatile metabolites of Pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry

“…A va- riety of volatile compounds are also formed by P. putrefaciens and P. fluorescens when incubated on sterile fish muscle and include the following: methyl mercaptan, DMDS, DMTS, and isopentanol (26). A detailed study of volatiles produced by P. putida and P. fluorescens isolated from spoiled, uncooked chicken and incubated on Trypticase soy agar identified 20 different compounds including DMDS, DMTS, 1-undecene, 2-butanone, and 2-nonanone, which were also identified in this study (22). P. aeruginosa when grown in culture produces a compound that imparts a grapelike odor to the culture and aids in the identification of this organism.…”

“…More recently, headspace analysis techniques have been developed to sample directly the volatile metabolites produced in culture. These techniques either have involved sampling the culture headspace directly, as in the case of aliphatic acids (2,21) and amines (21) for various anaerobes and sulfides for Proteus (14), or have made use of volatile concentration methods for Pityrosporum and pseudomonads (18,22,28). Headspace analysis has also been applied to samples of human body fluids including saliva (17), urine (25,39), and blood serum (24).…”

Characterization Of Ofice Dust By VOCs And TVOC Release - Identification Of Potential Irritant VOCs By Partial Least Squares Analysis

“…Most of the compounds with high Table 3 TVOC desorbed from particle fractions Ipg/gram dust calculated as decane equivalents), standard error in parentheses, average irritation index 1%) Gyntelberg et a1 (19931,and modeling power could arise either from (bio)-degradation of fatty acids or microbiological de novo synthesis (Wolkoff and Wilkens, 1993). Methyl ketones are known fatty acid degradation products by both bacteria and fungi (Cailleux et al, 1992;Kindelerer, 1987;Zechman and Labows, 1985;Lee et al, 1979). The unsaturated compound, 5-methyl-3-methylen-5-hexen-2-one could be an c1-or P-pinene oxidative degradation product similar to the previously identified 6-methyl-2-heptene-2-one (Ciccioli et al, 1992).…”

Indoor VOCs From Household Floor Dust: Comparison of Headspace with Desorbed VOCs; Method for VOC Release Determination

“…Larsson and Holst, 1982; 0. Lee et al, 1979; P. Rivers et al, 1992;Q. Seifert and King, 1982 ible source of fatty acids, saturated alkanals and 2alkanones is the degradation of fat during meat broiling and roasting (Rogge et al, 1991).…”