High-resolution genetic mapping of a novel brown planthopper resistance locus, Bph34 in Oryza sativa L. X Oryza nivara (Sharma &amp; Shastry) derived interspecific F2 population

Kumar, Kishor; Sarao, Preetinder Singh; Bhatia, Dharminder; Neelam, Kumari; Kaur, Amanpreet; Mangat, Gurjit Singh; Brar, D. S.; Singh, Kuldeep

doi:10.1007/s00122-018-3069-7

Cited by 77 publications

(41 citation statements)

References 50 publications

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“…However, improved cultivars carrying these genes lost their resistance to BPH due to the evolution of new biotypes (populations) [1,8,9,10,11,12]. At present, nine ( Bph3 , Bph6 , Bph9 , Bph14 , Bph17 , Bph18 , Bph26 , Bph29 , and Bph32 ) of the 34 BPH resistance genes ( Bph/bph ) have been cloned or characterized in rice and its relatives [13,14]. Of them, Bph3 (a cluster of plasma-membrane-localized lectin receptor kinases, OsLecRK s), has been considered for breeding rice cultivars with broad-spectrum and durable insect resistance [7,10].…”

Section: Introductionmentioning

confidence: 99%

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Nanda

Wan

Yuan

et al. 2018

IJMS

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The conserved mitogen-activated protein kinase (MAPK) cascades play vital roles in plant defense responses against pathogens and insects. In the current study, the expression profiles of 17 OsMPKs were determined in the TN1 and IR56 rice varieties under the infestation of brown planthopper (BPH), one of the most destructive hemimetabolous rice pests. The virulent IR56 BPH population (IR56-BPH) and the avirulent TN1 BPH population (TN-BPH) were used to reveal the roles of OsMPKs in the compatible (IR56-BPH infested on the TN1 and IR56 rice varieties, and TN1-BPH infested on the TN1 rice variety) and the incompatible (TN1-BPH infested on the IR56 rice variety) interaction. The statistical analysis revealed that rice variety, BPH population type, and infestation period have significant effects on the transcription of OsMPKs. Out of these genes, five OsMPKs (OsMPK1, OsMPK3, OsMPK7, OsMPK14, and OsMPK16) were found to exhibit upregulated expression only during incompatible interaction. Six OsMPKs (OsMPK4, OsMPK5, OsMPK8, OsMPK9, OsMPK12, and OsMPK13) were associated with both incompatible and compatible interactions. The transcription analysis of salicylic acid, jasmonic acid, and ethylene phytohormone signaling genes revealed their roles during the rice–BPH interactions. The upregulated expression of OsC4H, OsCHS, and OsCHI in the incompatible interaction implied the potential defense regulatory roles of phenylpropanoids. In both varieties, the elevated transcript accumulations of OsGST and OsSOD, and the increased enzyme activities of POD, SOD, and GST at 1 day post-infestation (dpi), but not at 3 dpi, indicated that reactive oxygen species (ROS) signaling might be an early event in rice–BPH interactions. Furthermore, upregulated transcription of OsLecRK3 and OsLecRK4 was found only during an incompatible interaction, suggesting their involvement in the BPH resistance response in the IR56 rice variety. Lastly, based on the findings of this study, we have proposed a model of interactions of IR56 rice with TN1-BPH and IR56-BPH that depicts the resistance and susceptibility reactions, respectively.

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Section: Introductionmentioning

confidence: 99%

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Nanda

Wan

Yuan

et al. 2018

IJMS

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“…For instance, thirteen genes are found in two distinct clusters of chromosome 4. Six of them ( Bph6 , bph12 , Bph27, bph18 ( t ) , Bph27 ( t ) , and Bph34 ) are clustered on the long arm of chromosome 4 (Qiu et al 2010, 2012; Huang et al 2013; Fujita et al 2013; Kumar et al 2018); and seven genes ( Bph3, Bph12, Bph15, Bph17, Bph20 ( t ), Bph30 , and Bph33 ) are found on the short arm of chromosome 4 (Liu et al, 2015; Yang et al 2002; Sun et al 2005; Yang et al 2004; Rahman et al 2009; Wang et al 2018; Hu et al 2018). According to the present study Bph36 also occupies this cluster, and via high-resolution mapping, it was successfully identified on the short arm of chromosome 4 roughly 2 Mb downstream from the Bph12, Bph17 , Bph15 , and Bph20 regions (Yang et al 2002; Sun et al 2005; Yang et al 2004; Rahman et al 2009) and 0.6 Mb upstream from Bph3 in the Nipponbare genome (Liu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff)

Xue

Zhou

et al. 2019

Rice

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Background The brown planthopper ( Nilaparvata lugens Stål; BPH), one of the most destructive pests of rice, has proven to be a substantial threat, conferring enormous production losses in Asia and becoming a difficult challenge to manipulate and control under field conditions. The continuous use of insecticides promotes the resurgence of BPH, which results in resistant varieties adapting through the upgrading of new BPH biotypes. To overcome resistance acquired by BPH against resistance varieties, different forms of novel resistant gene fusions act as functional domains for breeding to enhance insect resistance. Results The current study reports on the novel BPH resistance gene Bph36 derived from two introgression lines (RBPH16 and RBPH17) developed from wild rice GX2183 which was previously reported to be resistant to BPH. Using two F 2 crossing populations (Kangwenqizhan × RBPH16 and Huanghuazhan × RBPH17) in a bulked segregant analysis (BSA) for identification of resistant genes and QTL analysis, two QTLs for BPH resistance were generated on the long and short arms of chromosome 4, which was further confirmed by developing BC 1 F 2:3 populations by backcrossing via marker assisted selection (MAS) approach. One BPH resistance locus on the short arm of chromosome 4 was mapped to a 38-kb interval flanked by InDel markers S13 and X48, and then was named Bph36 , whereas another locus on the long arm of chromosome 4 was also detected in an interval flanked by RM16766 and RM17033, which was the same as that of Bph27 . An evaluation analysis based on four parameters (BPH host selection, honeydew weight, BPH survival rate and BPH population growth rate) shows that Bph36 conferred high levels antibiosis and antixenosis to BPH. Moreover, Bph36 pyramided with Bph3 , Bph27 , and Bph29 through MAS into elite cultivars 9311 and MH511 (harbored Xa23 ), creating different background breeding lines that also exhibited strong resistance to BPH in the seedling or tillering stage. Conclusion Bph36 can be utilized in BPH resistance breeding programs to develop high resistant rice lines and the high-resolution fine mapping will facilitate further map-based cloning and marker-assisted gene pyramiding of resistant gene. MAS exploited to pyramid with Bph3 , Bph27 , Bph29 , and Xa23 was confirmed the effectiveness for BPH resistance breeding in rice and provided insights into the molecular mechanism of defense to control this devastating insect. Electronic supplementary material ...

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“…Host‐plant resistance in rice is considered to be the most practical and economical approach for controlling rice planthoppers . In addition to the previously documented 84 major genes/quantitative trait loci for planthopper resistance in rice, at least 13 new major genes/quantitative trait loci for planthopper resistance in rice have been reported over the last 2 years, including Bph30 (AC‐1613), Bph31 , Bph32 (Rathu Heenati), Bph33 , Bph33 ( t ), Bph34 , qSBPH5 , qSBPH7 , qSBPH10 , qWBPH3.1 , qWBPH3.2 , qWBPH11 and qWBPH12 . Moreover, three unnamed recessive genes for BPH resistance have been reported from two newly identified rice germplasm sources (PHS29 and MRST3) .…”

Section: Molecular Players Of Rice Originmentioning

confidence: 99%

Current understanding of the molecular players involved in resistance to rice planthoppers

Yang

Zhang

2019

Pest Management Science

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Rice planthoppers are the most widespread and destructive pest of rice. Planthopper control depends greatly on the understanding of molecular players involved in resistance to planthoppers. This paper summarizes the recent progress in the understanding of some molecular players involved in resistance to planthoppers and the mechanisms involved. Recent researches showed that host‐plant resistance is the most promising sustainable approach for controlling planthoppers. Planthopper‐resistant varieties with a host‐plant resistance gene have been released for rice products. Integrated planthopper management is a proposed strategy to prolong the durability of host‐plant resistance. Bacillus spp. and their gene products or insect pathogenic fungi have great potential for application in the biological control of planthoppers. Enhancement of the activity of the natural enemies of planthoppers would be more cost‐effective and environmentally friendly. Various molecular processes regulate rice–planthopper interactions. Rice encounters planthopper attacks via transcription factors, secondary metabolites, and signaling networks in which phytohormones have central roles. Maintenance of cell wall integrity and lignification act as physical barriers. Indirect defenses of rice are regulated via chemical elicitors, honeydew‐associated elicitor, amendment with silicon and biochar, and salivary protein of BPH as elicitor or effector. Further research directions on planthopper control and rice defense against planthoppers are also put forward. © 2019 Society of Chemical Industry

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High-resolution genetic mapping of a novel brown planthopper resistance locus, Bph34 in Oryza sativa L. X Oryza nivara (Sharma & Shastry) derived interspecific F2 population

Cited by 77 publications

References 50 publications

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff)

Current understanding of the molecular players involved in resistance to rice planthoppers

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