High-resolution high-speed dynamic mechanical spectroscopy of cells and other soft materials with the help of atomic force microscopy

Dokukin, Maxim; Sokolov, Igor

doi:10.1038/srep12630

Cited by 41 publications

(32 citation statements)

References 57 publications

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“…This tendency for the PF-QNM mode seems to be a continuation of the tendency already observed for the FV mode on the Bioscope Catalyst. The decrease in elastic modulus against frequency for biological sample has already been reported by Dokukin and Sokolov (2015). They attributed this tendency to the creep relaxation.…”

Section: Effect Of the Tip Oscillation Frequencysupporting

confidence: 70%

High speed indentation measures by FV, QI and QNM introduce a new understanding of bionanomechanical experiments

Smolyakov

Formosa-Dague

Severac

et al. 2016

Micron

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Structural and mechanical mapping at the nanoscale by novel high-speed multiparametric Quantitative Imaging (QI) and PeakForce Quantitative Nanomechanical Mapping (PF-QNM) AFM modes was compared to the classical Force Volume (FV) mapping for the case of living Pseudomonas aeruginosa bacterial cells. QI and PF-QNM modes give results consistent with FV for the whole cells in terms of morphology and elastic modulus, while providing higher resolution and shorter acquisition time. As an important complement, the influence of scanning parameters on elastic modulus values was explored for small 0.2(2)μm(2) central area on top of cells. The modulus decreases with the indentation depth due to the effect of the hard cell wall, while it increases vs. tip oscillation frequency, displaying viscoelastic behaviour of the living bacterial cells. The ability of different AFM modes to follow correctly the bacteria viscoelastic behaviour at high oscillation frequency was tested.

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Section: Effect Of the Tip Oscillation Frequencysupporting

confidence: 70%

High speed indentation measures by FV, QI and QNM introduce a new understanding of bionanomechanical experiments

Smolyakov

Formosa-Dague

Severac

et al. 2016

Micron

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“…To enable a measurement within the range of linear material response, AFM-nDMA executes a force modulation while the AFM tip is in continuous contact ( Fig. 1 segment B), using a modulation force much smaller than the average force (or preload) 13,14 and with appropriate tip radius and force levels. 15 A non-resonant measurement is chosen to allow continuous frequency coverage in the rheologically relevant 0.1 Hz to 100 Hz frequency range.…”

Section: Dynamic Mechanical Analysis With Afm: Operating Principlesmentioning

confidence: 99%

Nanoscale DMA with the Atomic Force Microscope: A New Method for Measuring Viscoelastic Properties of Nanostructured Polymer Materials

Pittenger

Osechinskiy

Yablon³

et al. 2019

JOM

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We introduce nanoscale dynamic mechanical analysis (DMA) based on atomic force microscopy (AFM), a new mode for quantitative viscoelastic analysis of heterogeneous polymer materials at the nanoscale (AFM-nDMA). AFM-nDMA takes advantage of the exquisite force sensitivity, small contact radius, and nanoscale indentation depth of the AFM to provide dynamic mechanical analysis with 10 nm spatial resolution at rheologically relevant frequencies and variable temperature. This novel, non-resonant measurement is embedded in a force curve and typically occurs at a series of frequencies to provide spectra of storage modulus, loss modulus, and loss tangent. By using tailored probes and mitigating the effect of contact radius changes throughout the measurement, quantitative results are obtained that tie directly to bulk DMA and allow for time-temperature superposition analysis. The combination of quantitative results and 10 nm spatial resolution holds promise for the investigation of previously inaccessible microscopic domains, confinement effects, and interphases.

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“…in the quasi-static regime. By exciting the sample via a piezo-scanner it is possible to measure more rapidly the mechanical characteristic of samples at multiple frequencies up to 300 Hz, with a sensible advantage in the measurement time with respect to commercial nanoindenters 2 . In both cases the frequency is limited to the sub-kHz regime.…”

Section: Discussionmentioning

confidence: 99%

Theory of Single-Impact Atomic Force Spectroscopy in liquids with material contrast

López-Guerra

Banfi

Solares

et al. 2018

Sci Rep

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Scanning probe microscopy has enabled nanoscale mapping of mechanical properties in important technological materials, such as tissues, biomaterials, polymers, nanointerfaces of composite materials, to name only a few. To improve and widen the measurement of nanoscale mechanical properties, a number of methods have been proposed to overcome the widely used force-displacement mode, that is inherently slow and limited to a quasi-static regime, mainly using multiple sinusoidal excitations of the sample base or of the cantilever. Here, a different approach is put forward. It exploits the unique capabilities of the wavelet transform analysis to harness the information encoded in a short duration spectroscopy experiment. It is based on an impulsive excitation of the cantilever and a single impact of the tip with the sample. It performs well in highly damped environments, which are often seen as problematic in other standard dynamic methods. Our results are very promising in terms of viscoelastic property discrimination. Their potential is oriented (but not limited) to samples that demand imaging in liquid native environments and also to highly vulnerable samples whose compositional mapping cannot be obtained through standard tapping imaging techniques.

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High-resolution high-speed dynamic mechanical spectroscopy of cells and other soft materials with the help of atomic force microscopy

Cited by 41 publications

References 57 publications

High speed indentation measures by FV, QI and QNM introduce a new understanding of bionanomechanical experiments

High speed indentation measures by FV, QI and QNM introduce a new understanding of bionanomechanical experiments

Nanoscale DMA with the Atomic Force Microscope: A New Method for Measuring Viscoelastic Properties of Nanostructured Polymer Materials

Theory of Single-Impact Atomic Force Spectroscopy in liquids with material contrast

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