High resolution laboratory grating-based X-ray phase-contrast CT

Viermetz, Manuel; Birnbacher, Lorenz; Willner, Marian; Achterhold, Klaus; Pfeiffer, Franz; Herzen, Julia

doi:10.1038/s41598-018-33997-5

Cited by 27 publications

(18 citation statements)

References 41 publications

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“…Our results show that tissue characterisation is feasible at both imaging setups and signal intensities for different tissue types is similar. In addition, using advanced imaging and reconstruction techniques, Viermetz et al significantly improved spatial resolution of grating-based PCCT using conventional X-ray sources 21 22. We did not investigate the effect of formalin fixation on image contrast and signal intensities in PCCT.…”

Section: Discussionmentioning

confidence: 96%

Grating-based phase-contrast CT (PCCT): histopathological correlation of human liver cirrhosis and hepatocellular carcinoma specimen

et al. 2020

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AimsTo correlate signal intensities in grating-based phase-contrast CT (PCCT) images obtained at a synchrotron light source and a conventional X-ray source with tissue components in human liver cirrhosis and hepatocellular carcinoma (HCC) specimen.MethodsStudy approval was obtained by the institutional review board. Human specimen of liver cirrhosis and HCC were imaged at experimental grating-based PCCT setups using either a synchrotron radiation source or a conventional X-ray tube. Tissue samples were sectioned and processed for H&E and Elastica van Gieson staining. PCCT and histological images were manually correlated. Depending on morphology and staining characteristics tissue components like fibrosis, HCC, inflammation, connective tissue and necrosis were differentiated and visually correlated with signal intensity in PCCT images using a 5-point Likert scale with normal liver parenchyma as a reference.ResultsGrating-based PCCT images of human cirrhotic liver and HCC specimen showed high soft-tissue contrast allowing correlation with histopathological sections. Signal intensities were similar in both setups independent of the nature of the radiation source. Connective tissue and areas of haemorrhage displayed the highest signal intensities, fibrotic liver tissue the lowest.ConclusionsGrating-based PCCT provides comparable results for the characterisation of human specimen of liver cirrhosis and HCC using either a synchrotron light source or a conventional X-ray tube. Due to its high soft-tissue contrast and its applicability to conventional X-ray tubes grating-based PCCT holds potential for preclinical research and virtual histology applications.

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Section: Discussionmentioning

confidence: 96%

Grating-based phase-contrast CT (PCCT): histopathological correlation of human liver cirrhosis and hepatocellular carcinoma specimen

et al. 2020

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“…Micro-CTs with X-PCI capabilities can be expected in the near future. 34,35,36,37,38,39,40 Also, while studies on imaging beating hearts (Langendorff and in-vivo) with X-PCI are underway, the current research was performed on static, ex-vivo hearts thus lacking direct correlative function assessment, and lacking direct comparison with in vivo methods.…”

Section: Limitationsmentioning

confidence: 99%

Myocardial Remodeling in Ischemic Heart Disease – Imaging from Cell to Organ Level by Synchrotron Propagation Based X-Ray Phase-Contrast Imaging

Planinc

García-Cañadilla

Dejea

et al. 2021

Preprint

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Background: Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail.The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction.Methods and Results: Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8µm and 0.65µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas.Conclusions: Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.

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“…Another approach to enhance spatial resolution is a super-resolution (SR) imaging technique. The main advantage of SR techniques is that the SR image reconstruction allows to generate high resolution (HR) images from experimentally measured multiple low resolution (LR) images without changing the hardware 16–25 .…”

Section: Introductionmentioning

confidence: 99%

Super-Resolution Scanning Transmission X-Ray Imaging Using Single Biconcave Parabolic Refractive Lens Array

Mamyrbayev

Ikematsu

Meyer

et al. 2019

Sci Rep

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A new super resolution imaging technique which potentially enables sub-µm spatial resolution, using a detector of pixels much larger than the spatial resolution, is proposed. The method utilizes sample scanning through a large number of identical X-ray microprobes periodically spaced (the period corresponds to a multiple of the pixel size), which reduces drastically the scanning time. The information about the sample illuminated by the microprobes is stored by large detector pixels. Using these data and sample position information, a super-resolution image reconstruction is performed. With a one-dimensional (1D) high aspect ratio nickel single lens array designed for theoretically expected sub-µm microprobes at 17 keV and fabricated by deep X-ray lithography and electroforming technique, 2 µm X-ray microprobes with a period of 10 µm were achieved. We performed a first experiment at KARA synchrotron facility, and it was demonstrated that the smallest structure of a test pattern with a size of 1.5 µm could be easily resolved by using images generated from a detector having a pixel size of 10.4 µm. This new approach has a great potential for providing a new microscopic imaging modality with a large field of view and short scan time.

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High resolution laboratory grating-based X-ray phase-contrast CT

Cited by 27 publications

References 41 publications

Grating-based phase-contrast CT (PCCT): histopathological correlation of human liver cirrhosis and hepatocellular carcinoma specimen

Grating-based phase-contrast CT (PCCT): histopathological correlation of human liver cirrhosis and hepatocellular carcinoma specimen

Myocardial Remodeling in Ischemic Heart Disease – Imaging from Cell to Organ Level by Synchrotron Propagation Based X-Ray Phase-Contrast Imaging

Super-Resolution Scanning Transmission X-Ray Imaging Using Single Biconcave Parabolic Refractive Lens Array

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