High-resolution lipidomics reveals dysregulation of lipid metabolism in respiratory syncytial virus pneumonia mice

Shan, Jinjun; Qian, Wenjuan; Shen, Cunsi; Lin, Lili; Xie, Ting; Peng, Linxiu; Xu, Jian; Yang, Rui; Ji, Jianjian; Zhao, Xia

doi:10.1039/c8ra05640d

Cited by 44 publications

(46 citation statements)

References 41 publications

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“…In addition, we have also identified plasmalogen lipids. These lipids can prevent lung disease due to their antioxidant effects, as the lung is a direct target of reactive oxidant species [24]. In this study, we found that the concentration of plasmalogen lipids decreased.…”

Section: Discussionsupporting

confidence: 48%

“…It is possible to find new targets for the treatment of diseases if a deeper understanding of the relationship between lipid composition and lung disease is acquired [23]. Lipidomics is a rapidly evolving tool that explores the potential lipid biomarkers in diseases and the biological functions of lipids in various life activities by comparing the changes in lipid metabolism networks under different physiological conditions [24].…”

Section: Discussionmentioning

confidence: 99%

“…DPPC can inhibit cytokine production induced by airway epithelial cells and LPS, potentially by incorporation into the plasma membrane and affecting cell membrane fluidity. In addition, intratracheal DPPC supplementation in mice attenuates lung inflammation induced by intravenous LPS [22,24]. Another major phospholipid component is PG, which plays a role in stabilizing the alveolar tension, and in regulating the innate immune response [26].…”

Section: Discussionmentioning

confidence: 99%

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Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry

et al. 2019

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Surfactant lipid metabolism is closely related to pulmonary diseases. Lipid metabolism disorder can cause lung diseases, vice versa. With this rationale, a useful method was established in this study to determine the lipidome in bronchoalveolar lavage fluid (BALF) of mice. The lipid components in BALF were extracted by liquid–liquid extraction (methanol and methyl tert-butyl ether, and water). Ultra-high-performance liquid chromatography coupled to hybrid Quadrupole-Exactive Orbitrap mass spectrometry was used to analyze the extracted samples, which showed a broad scanning range of 215–1800 m/z. With MS-DIAL software and built-in LipidBlast database, we identified 38 lipids in positive, and 31 lipids in negative, ion mode, including lysophosphatidylcholine (lysoPC), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), etc. Then, the changes of lipids in BALF of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) was investigated, which may contribute to further exploration of the pathogenesis of ALI.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry

et al. 2019

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“…This, and the PICRUSt data in our study, suggest that increased sphingosine metabolism following RSV infection may be due to a change in gut microbiota metabolism. Sphingolipid metabolite abundance has also been observed to be altered in the lung metabolome after RSV infection in mice (Shan et al, 2018) and in the airways of children with RSV-bronchiolitis (Stewart et al, 2017). It is interesting to speculate whether altered gut metabolites promote host resistance to the infection or tolerance to the immunopathology (Wang et al, 2016;Trompette et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Respiratory viral infection alters the gut microbiota by inducing inappetence

Groves

Higham

Moffatt

et al. 2019

Preprint

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The gut microbiota has an important role in health and disease. Respiratory viral infections are extremely common but their impact on the composition and function of the gut microbiota is poorly understood. We previously observed a significant change in the gut microbiota after viral lung infection. Here we show that weight loss during Respiratory Syncytial Virus (RSV) or influenza virus infection was due to decreased food consumption, and that fasting mice independently of infection altered gut microbiota composition. While the acute phase TNF-α response drove early weight loss and inappetence during RSV infection, this was not sufficient to induce changes in the gut microbiota. However, depleting CD8 + cells increased food intake and prevented weight loss resulting in a reversal of the gut microbiota changes normally observed during RSV infection. Viral infection also led to changes in the faecal gut metabolome during RSV infection, with a significant shift in lipid metabolism. Sphingolipids, poly-unsaturated fatty acids (PUFAs) and the short-chain fatty acid (SCFA) valerate all increased in abundance in the faecal metabolome following RSV infection. Whether this, and the impact of infection-induced anorexia on the gut microbiota, are part of a protective, anti-inflammatory response during respiratory viral infections remains to be determined.

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“…Data raw files (.wiff) were converted into .abf format in order to perform retention time correction, peak alignment, and identification. The latter was achieved by comparison of experimental spectra with those in the LipidBlast library [24], using both accurate mass and MS/MS fragmentation (Table S1) data (total identification score >70%). Prevalent adducts were previously investigated in our experimental conditions, and thus, the identification was restricted only on them.…”

Section: Lipidomic Data Processingmentioning

confidence: 99%

An Innovative Lipidomic Workflow to Investigate the Lipid Profile in a Cystic Fibrosis Cell Line

Dei

Zulueta

Mingione

et al. 2020

Cells

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Altered lipid metabolism has been associated to cystic fibrosis disease, which is characterized by chronic lung inflammation and various organs dysfunction. Here, we present the validation of an untargeted lipidomics approach based on high-resolution mass spectrometry aimed at identifying those lipid species that unequivocally sign CF pathophysiology. Of n.13375 mass spectra recorded on cystic fibrosis bronchial epithelial airways epithelial cells IB3, n.7787 presented the MS/MS data, and, after software and manual validation, the final number of annotated lipids was restricted to n.1159. On these lipids, univariate and multivariate statistical approaches were employed in order to select relevant lipids for cellular phenotype discrimination between cystic fibrosis and HBE healthy cells. In cystic fibrosis IB3 cells, a pervasive alteration in the lipid metabolism revealed changes in the classes of ether-linked phospholipids, cholesterol esters, and glycosylated sphingolipids. Through functions association, it was evidenced that lipids variation involves the moiety implicated in membrane composition, endoplasmic reticulum, mitochondria compartments, and chemical and biophysical lipids properties. This study provides a new perspective in understanding the pathogenesis of cystic fibrosis and strengthens the need to use a validated mass spectrometry-based lipidomics approach for the discovery of potential biomarkers and perturbed metabolism.

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High-resolution lipidomics reveals dysregulation of lipid metabolism in respiratory syncytial virus pneumonia mice

Abstract: Respiratory syncytial virus (RSV) is a leading viral pathogen responsible for lower respiratory tract infections, particularly in children under five years worldwide, often resulting in hospitalization.

Cited by 44 publications

References 41 publications

Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry

Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry

Respiratory viral infection alters the gut microbiota by inducing inappetence

An Innovative Lipidomic Workflow to Investigate the Lipid Profile in a Cystic Fibrosis Cell Line

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