High-resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease

Pidon, Hélène; Wendler, Neele; Habekuβ, Antje; Maasberg, Anja; Ruge-Wehling, Brigitte; Perović, Dragan; Ordon, Frank; Stein, Nils

doi:10.1007/s00122-020-03733-7

Cited by 15 publications

(13 citation statements)

References 76 publications

(100 reference statements)

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“…In the F 2 populations, we analyzed a currently used method based on finger rubbing for mechanical inoculation which revealed about 10% escapes. However, the efficiency in the present study was much higher than in similar studies (Shi et al 2019;Pidon et al 2020), although a similar inoculation method (Habekuß et al 2008) was used.…”

Section: Discussioncontrasting

confidence: 59%

Delineating the elusive BaMMV resistance gene rym15 in barley by medium-resolution mapping

et al. 2021

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Barley mild mosaic virus (BaMMV), transmitted by the soil-borne protist Polymyxa graminis, has a serious impact on winter barley production. Previously, the BaMMV resistance gene rym15 was mapped on chromosome 6HS, but the order of flanking markers was non-collinear between different maps. To resolve the position of the flanking markers and to enable map-based cloning of rym15, two medium-resolution mapping populations Igri (susceptible) × Chikurin Ibaraki 1 (resistant) (I × C) and Chikurin Ibaraki 1 × Uschi (susceptible) (C × U), consisting of 342 and 180 F2 plants, respectively, were developed. Efficiency of the mechanical inoculation of susceptible standards varied from 87.5 to 100% and in F2 populations from 90.56 to 93.23%. Phenotyping of F2 plants and corresponding F3 families revealed segregation ratios of 250 s:92r (I × C, χ2 = 0.659) and 140 s:40r (C × U, χ2 = 0.741), suggesting the presence of a single recessive resistance gene. After screening the parents with the 50 K Infinium chip and anchoring corresponding SNPs to the barley reference genome, 8 KASP assays were developed and used to remap the gene. Newly constructed maps revealed a collinear order of markers, thereby allowing the identification of high throughput flanking markers. This study demonstrates how construction of medium-resolution mapping populations in combination with robust phenotyping can efficiently resolve conflicting marker ordering and reduce the size of the target interval. In the reference genome era and genome-wide genotyping era, medium-resolution mapping will help accelerate candidate gene identification for traits where phenotyping is difficult.

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Section: Discussioncontrasting

confidence: 59%

Delineating the elusive BaMMV resistance gene rym15 in barley by medium-resolution mapping

et al. 2021

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“…In addition to cloned eIF4E and PDIL5-1 , rym7 [ 26 ], Rym16 HB [ 27 , 28 ], Rym17 [ 29 ] and Rym14 HB [ 4 ] have been fine-mapped to a small region on chromosome 1H, 2H, 3H, and 6H, respectively. The remaining 10 BYMD-resistant genes rym8 [ 30 ], rym9 [ 31 ], rym12 [ 32 ], rym13 [ 33 ], rym18 [ 29 ], rym3 [ 34 ], Un-designated [ 35 ], rym15 [ 36 ], rym2 [ 32 ] and rym7t [ 37 ] have been preliminarily mapped.…”

Section: Backgroundsmentioning

confidence: 99%

Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

et al. 2021

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Background Barley yellow mosaic disease (BYMD) caused by Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) seriously threatens the production of winter barley. Cultivating and promoting varieties that carry disease-resistant genes is one of the most powerful ways to minimize the disease’s effect on yield. However, as the BYMD virus mutates rapidly, resistance conferred by the two cloned R genes to the virus had been overcome by new virus strains. There is an urgent need for novel resistance genes in barley that convey sustainable resistance to newly emerging virus strains causing BYMD. Results A doubled haploid (DH) population derived from a cross of SRY01 (BYMD resistant wild barley) and Gairdner (BYMD susceptible barley cultivar) was used to explore for QTL of resistance to BYMD in barley. A total of six quantitative trait loci (qRYM-1H, qRYM-2Ha, qRYM-2Hb, qRYM-3H, qRYM-5H, and qRYM-7H) related to BYMD resistance were detected, which were located on chromosomes 1H, 2H, 3H, 5H, and 7H. Both qRYM-1H and qRYM-2Ha were detected in all environments. qRYM-1H was found to be overlapped with rym7, a known R gene to the disease, whereas qRYM-2Ha is a novel QTL on chromosome 2H originated from SRY01, explaining phenotypic variation from 9.8 to 17.8%. The closely linked InDel markers for qRYM-2Ha were developed which could be used for marker-assisted selection in barley breeding. qRYM-2Hb and qRYM-3H were stable QTL for specific resistance to Yancheng and Yangzhou virus strains, respectively. qRYM-5H and qRYM-7H identified in Yangzhou were originated from Gairdner. Conclusions Our work is focusing on a virus disease (barley yellow mosaic) of barley. It is the first report on BYMD-resistant QTL from wild barley accessions. One novel major QTL (qRYM-2Ha) for the resistance was detected. The consistently detected new genes will potentially serve as novel sources for achieving pre-breeding barley materials with resistance to BYMD.

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“…However, the disease resistance of rym1/11 has already been overcome by new BaYMV strains in Japan ( Okada et al, 2004 ). In addition to the cloned eIF4E and PDIL5-1 resistance alleles, rym7 (resistance against BaMMV) ( Yang et al, 2013 ), Rym16 Hb (from Hordeum bulbosum , resistance against all strains) ( Ruge-Wehling et al, 2006 ; Johnston et al, 2015 ), Rym17 (from barley landrace, resistance against BaYMV) ( Kai et al, 2012 ) and Rym14 Hb (from Hordeum bulbosum , resistance against all strains) ( Pidon et al, 2020 ) have been precisely mapped to small regions on chromosomes 1H, 2H, 3H, and 6H, respectively. The remaining ten BaYMV disease resistance genes rym2 (resistance against several strains of the two viruses) ( Gotz & Friedt, 1993 ), rym3 (resistance against several BaYMV strains) ( Werner et al, 2003b ), rym7t (resistance against several BaYMV strains) ( Takata et al, 2012 ), rym8 (resistance against several strains of the two viruses) ( Bauer et al, 1997 ), rym9 (resistance against several BaMMV strains) ( Werner, Friedt & Ordon, 2005 ), rym12 (resistance against several strains of the two viruses) ( Gotz & Friedt, 1993 ), rym13 (resistance against several strains of the two viruses) ( Werner et al, 2003a ), rym15 (resistance against BaMMV) ( Le Gouis et al, 2004 ), rym18 (resistance against BaYMV) ( Kai et al, 2012 ), and Un-designated (resistance against BaYMV) ( Saeki et al, 1999 ) have only been preliminarily mapped.…”

Section: Introductionmentioning

confidence: 99%

Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

Pan

Jun

Hong

et al. 2022

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Background The disease caused by Barley yellow mosaic virus (BaYMV) infection is a serious threat to autumn-sown barley (Hordeum vulgare L.) production in Europe, East Asia and Iran. Due to the rapid diversification of BaYMV strains, it is urgent to discover novel germplasm and genes to assist breeding new varieties with resistance to different BaYMV strains, thus minimizing the effect of BaYMV disease on barley cropping. Methods A natural population consisting of 181 barley accessions with different levels of resistance to BaYMV disease was selected for field resistance identification in two separate locations (Yangzhou and Yancheng, Jiangsu Province, China). Additive main effects and multiplicative interaction (AMMI) analysis was used to identify accessions with stable resistance. Genome-wide association study (GWAS) of BaYMV disease resistance was broadly performed by combining both single nucleotide polymorphisms (SNPs) and specific molecular markers associated with the reported BaYMV disease resistance genes. Furthermore, the viral protein genome linked (VPg) sequences of the virus were amplified and analyzed to assess the differences between the BaYMV strains sourced from the different experimental sites. Results Seven barley accessions with lower standardized Area Under the Disease Progress Steps (sAUDPS) index in every environment were identified and shown to have stable resistance to BaYMV disease in each assessed location. Apart from the reported BaYMV disease resistance genes rym4 and rym5, one novel resistance locus explaining 24.21% of the phenotypic variation was identified at the Yangzhou testing site, while two other novel resistance loci that contributed 19.23% and 19.79% of the phenotypic variation were identified at the Yancheng testing site, respectively. Further analysis regarding the difference in the VPg sequence of the predominant strain of BaYMV collected from these two testing sites may explain the difference of resistance loci differentially identified under geographically distinct regions. Our research provides novel genetic resources and resistance loci for breeding barley varieties for BaMYV disease resistance.

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High-resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease

Cited by 15 publications

References 76 publications

Delineating the elusive BaMMV resistance gene rym15 in barley by medium-resolution mapping

Delineating the elusive BaMMV resistance gene rym15 in barley by medium-resolution mapping

Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

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