High-resolution mapping of the Mov-1 locus in wheat by combining radiation hybrid (RH) and recombination-based mapping approaches

Mahlandt, Alexander; Rawat, Nidhi; Leonard, Jeffrey M.; Venglat, Prakash; Datla, Raju; Meier, Nathan; Gill, Bikram S.; Riera‐Lizarazu, Oscar; Coleman, Gary D.; Murphy, Angus S.; Tiwari, Vijay K.

doi:10.1007/s00122-021-03827-w

Cited by 6 publications

(22 citation statements)

References 52 publications

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“…Given that TRP was a single dominant gene, additionally, the 5A locus was only detected in one of the replicate penetrance traits (Supplemental Table 4). Thus, the 5A locus may have been a pseudo-locus, since it Mapping results were consistent with previous studies (Mahlandt et al 2021;Yu et al 2020).…”

Section: Genetic Analysis Of Trpsupporting

confidence: 90%

“…1a, Supplemental Table 1). In addition, our unpublished data showed that kernel weight of 4 F 1 populations increased more than 2 folds, compared to the normal parental wheat line, which was also found by Mahlandt et al (2021). These observations imply the enormous potential of TRP in enhancing yields and development of wheat hybrid-breeding.…”

Section: Discussionmentioning

confidence: 52%

“…7). Subsequently, Mahlandt et al (2021) physically located Mov-1 to a 1.1 Mb region by combining radiation hybrid (RH) and recombination-based mapping approaches (Supplemental Fig. 7).…”

Section: Discussionmentioning

confidence: 99%

“…Within the past fty years, studies evaluated oral morphologies, genetic make-up, biochemical features, and gene mapping of similar tri-pistil mutants, which were subsequently referred to as "multi-ovary" (MOV), "multiple-pistil" (MP), and "three-pistil" (TP) (Chen et Yu et al 2020). The tri-pistil mutants generate normal stamens and other oral organs, except for three fertile pistils (Li et al 2020c; Mahlandt et al 2021;Peng 2003). When crossed with normal lines, all F 1 plants from different cross groups exhibited a tri-pistil trait.…”

Section: Introductionmentioning

confidence: 99%

“…Mahlandt et al 2021;Yu et al 2020). In addition, poor penetrance of most 4045 F 1 populations, which differed from previous studies, implied that TRP may be different from Pis1 or Mov-1.Map-based cloning is an important strategy for gene identi cation in wheat, whereas it was di cult and time-consuming before the release of wheat genomes.…”

mentioning

confidence: 99%

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Morphological characterization and genetic analysis of tri-pistil trait, a precisely regulated pistil number mutation in bread wheat

Liu

Sun

et al. 2022

Preprint

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Bread wheat (Triticum aestivum L.) is an important source of nutrients for humans. Therefore, improvement of its yields is essential to feed the increasing world population. The tri-pistil (TRP) trait in wheat has a high potential for increasing yields. We obtained a pure tri-pistil wheat line, 4045, and evaluated its morphological properties. The 4045 wheat line stably produced three independently inherited pistils, which led to 1-3 grains in each floret. Among the three pistils, two lately emerged pistils initiated at late anther primordia stage to early tetrads stage. Genetic analysis revealed that there were TRP penetrance variations among the 11 F1 populations of 4045. Fine mapping narrowed the single dominant TRP locus to a 97.3 kb region, containing two candidate genes, on the 2DL chromosome. However, further gene sequence, functional as well as comparative genomic analyses ruled out the only two candidate genes. Therefore, TRP is high-likely a unique gain-of-function mutation that does not exist in normal wheat genome. Transcriptome analysis of floral homeotic genes revealed that expressions of the C-class TaAG-2s, which are essential for carpel specification, significantly increased in 4045, implying that TaAG-2s have played important roles in TRP-regulated tri-pistil formation. This study highlights that TRP leads to a precisely regulated pistil number increase (PRPNI) mutations and proposed a regulatory model of PRPNI pistil architecture. PRPNI offers a novel abnormal pistil development resource for research of floral architectures and potential on crop yield improvement.

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Section: Genetic Analysis Of Trpsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 52%

“…7). Subsequently, Mahlandt et al (2021) physically located Mov-1 to a 1.1 Mb region by combining radiation hybrid (RH) and recombination-based mapping approaches (Supplemental Fig. 7).…”

Section: Discussionmentioning

confidence: 99%