High-resolution mapping on pachytene chromosomes and extended DNA fibres by fluorescencein-situ hybridisation

Zhong, Xiao‐bo; Fransz, Paul; Eden, J. van; Zabel, P.; Kammen, A. van; Jong, J.H. de

doi:10.1007/bf02671658

Cited by 88 publications

(55 citation statements)

References 17 publications

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“…Due to the difficulty in obtaining clearly delineated chromosome spreads, it was difficult to ascertain the precise order of the FISH signals on the putative homeolog although the three most-distal BACs (161_O23, 038_L01, and 144_N07) are colinear (Figure 1, A and D, insets). DISCUSSION FISH is a common approach to merge genetic, physical, and chromosome maps to gain insight into the organization and structure of a genome (Arabidopsis, Zhong et al 1996 andJackson et al 1998;rice, Cheng et al 2001;humans, Cheung et al 2001; Medicago truncatula, Kulikova et al 2001). BAC clones, either genetically anchored or unanchored, can be used as FISH probes to map individual BACs or BAC contigs to the chromosomes, thereby integrating the physical map, and often the genetic map, with specific chromosomes and/or chromosomal regions.…”

Section: Resultsmentioning

confidence: 99%

Chromosome-Level Homeology in Paleopolyploid Soybean (Glycine max) Revealed Through Integration of Genetic and Chromosome Maps

et al. 2006

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Soybean has 20 chromosome pairs that are derived from at least two rounds of genomewide duplication or polyploidy events although, cytogenetically, soybean behaves like a diploid and has disomic inheritance for most loci. Genetically anchored genomic clones were used as probes for fluorescence in situ hybridization (FISH) to determine the level of postpolyploid chromosomal rearrangements and to integrate the genetic and physical maps to (1) assign linkage groups to specific chromosomes, (2) assess chromosomal structure, and (3) determine the distribution of recombination along the length of a chromosome. FISH mapping of seven putatively gene-rich BACs from linkage group L (chromosome 19) revealed that most of the genetic map correlates to the highly euchromatic long arm and that there is extensive homeology with another chromosome pair, although colinearity of some loci does appear to be disrupted. Moreover, mapping of BACs containing high-copy sequences revealed sequestration of high-copy repeats to the pericentromeric regions of this chromosome. Taken together, these data present a model of chromosome structure in a highly duplicated but diploidized eukaryote, soybean.

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Section: Resultsmentioning

confidence: 99%

Chromosome-Level Homeology in Paleopolyploid Soybean (Glycine max) Revealed Through Integration of Genetic and Chromosome Maps

et al. 2006

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“…After removal of cover glasses in 23 SSC and two 5-min washes with 23 SSC, the slides were washed three times for 5 min each in 50% formamide in 23 SSC at 42°(80% stringency). Blocking and antibody incubations were performed in 1-hr increments at 37°as described (Zhong et al 1996;Chang 2004). The antibodies differed depending on the probe label and included (in this order but not necessarily in each experiment) mouse anti-biotin 1:100 (Roche), biotinylated donkey anti-mouse 1:250 ( Jackson ImmunoResearch) and/or sheep anti-DIG tetramethyl rhodamine (TRITC) 1:125 (Roche), and streptavidin-FITC 1:250 (Roche) and/or donkey anti-sheep-TRITC 1:100 ( Jackson ImmunoResearch).…”

Section: Methodsmentioning

confidence: 99%

“…FISH was performed as described (Zhong et al 1996;Chang 2004) with the following modifications. Glass microscope slides with SC spreads were incubated for 1 min each in 45% acetic acid, freshly prepared 1:3 acetic ethanol, and 100% ethanol before air drying for 30 min at 65°.…”

Section: Methodsmentioning

confidence: 99%

Predicting and Testing Physical Locations of Genetically Mapped Loci on Tomato Pachytene Chromosome1

Chang¹,

Anderson

Sherman

et al. 2007

Genetics

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Predicting the chromosomal location of mapped markers has been difficult because linkage maps do not reveal differences in crossover frequencies along the physical structure of chromosomes. Here we combine a physical crossover map based on the distribution of recombination nodules (RNs) on Solanum lycopersicum (tomato) synaptonemal complex 1 with a molecular genetic linkage map from the interspecific hybrid S. lycopersicum 3 S. pennellii to predict the physical locations of 17 mapped loci on tomato pachytene chromosome 1. Except for one marker located in heterochromatin, the predicted locations agree well with the observed locations determined by fluorescence in situ hybridization. One advantage of this approach is that once the RN distribution has been determined, the chromosomal location of any mapped locus (current or future) can be predicted with a high level of confidence.

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“…Fluorescence in situ hybridization, microscopy, and image processing: Fixed anthers were digested in a pectolytic enzyme mixture, transferred to a clean slide, and spread according to Zhong et al (1996). This procedure involves no mechanical pressure to spread the cells on the slide and the threedimensional information is largely preserved.…”

Section: Plant Materialmentioning

confidence: 99%

Terminal Regions of Wheat Chromosomes Select Their Pairing Partners in Meiosis

et al. 2007

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Many plant species, including important crops like wheat, are polyploids that carry more than two sets of genetically related chromosomes capable of meiotic pairing. To safeguard a diploid-like behavior at meiosis, many polyploids evolved genetic loci that suppress incorrect pairing and recombination of homeologues. The Ph1 locus in wheat was proposed to ensure homologous pairing by controlling the specificity of centromere associations that precede chromosome pairing. Using wheat chromosomes that carry rye centromeres, we show that the centromere associations in early meiosis are not based on homology and that the Ph1 locus has no effect on such associations. Although centromeres indeed undergo a switch from nonhomologous to homologous associations in meiosis, this process is driven by the terminally initiated synapsis. The centromere has no effect on metaphase I chiasmate chromosome associations: homologs with identical or different centromeres, in the presence and absence of Ph1, pair the same. A FISH analysis of the behavior of centromeres and distal chromomeres in telocentric and biarmed chromosomes demonstrates that it is not the centromeric, but rather the subtelomeric, regions that are involved in the correct partner recognition and selection.

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High-resolution mapping on pachytene chromosomes and extended DNA fibres by fluorescencein-situ hybridisation

Cited by 88 publications

References 17 publications

Chromosome-Level Homeology in Paleopolyploid Soybean (Glycine max) Revealed Through Integration of Genetic and Chromosome Maps

Chromosome-Level Homeology in Paleopolyploid Soybean (Glycine max) Revealed Through Integration of Genetic and Chromosome Maps

Predicting and Testing Physical Locations of Genetically Mapped Loci on Tomato Pachytene Chromosome1

Terminal Regions of Wheat Chromosomes Select Their Pairing Partners in Meiosis

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