High resolution (NA=08) in lensless in-line holographic microscopy with glass sample carriers

Kanka, Mario; Riesenberg, Rainer; Petruck, P.; Graulig, Christian

doi:10.1364/ol.36.003651

Cited by 42 publications

(29 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently another configuration was also reported [16] for NA=0.8 in DIHM, achieved with air between the pinhole and sample to visualize a large area from the sample. The values for NA are inverse proportional with the pinhole diameter.…”

Section: Compact System Designmentioning

confidence: 99%

Compact system design based on digital in-line holographic microscopy configuration

Mihăilescu¹,

Kusko²

2012

J. Eur. Opt. Soc.-Rapid Publ.

View full text Add to dashboard Cite

We present our study regarding a compact system design for cell counting and simultaneous 3D imaging, based on digital in-line holographic microscopy configuration. The system is built around the known experimental configuration which includes a pinhole but we also investigate the configuration with a monomode fiber as a light source. Considered samples consist of a very low concentration of cells in flow in a microchannel. The main challenge in our design is to obtain the digital hologram of one cell on a regular video camera sensor in proper resolution conditions, as opposed to the usual configurations where the aim is to visualize a large area. This fact is possible with shorter distances between pinhole and sample and with pinholes with diameters slightly larger than 1micron. These can now be realized by considering the microtechnological processes for microchannel and pinhole fabrication on the same substrate with high refractive indexto increase the numerical aperture of the system The geometrical parameters are established after the numerical analysis of the diffracted field from a single cell and of the entire system numerical aperture values.

show abstract

Section: Compact System Designmentioning

confidence: 99%

Compact system design based on digital in-line holographic microscopy configuration

Mihăilescu¹,

Kusko²

2012

J. Eur. Opt. Soc.-Rapid Publ.

View full text Add to dashboard Cite

show abstract

“…Although computational approaches involving pixel super-resolution 18,21 and pixel function estimation or measurement 23 can partially help to boost some of these spatial frequencies, on-chip microscopy has thus far been limited to an NA of less than about 0.8-0.9. 11,23,30 Synthetic aperture approaches in optical microscopy [31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] were originally implemented to overcome the limited SBPs of traditional lensbased imaging designs. Here, we demonstrate the first application of the synthetic aperture technique in lensfree holographic on-chip imaging to reach a record high NA of 1.4 over a large FOV of .20 mm 2 , where the sample is sequentially illuminated at various angles using a partially coherent light source (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Synthetic aperture-based on-chip microscopy

et al. 2015

View full text Add to dashboard Cite

Wide field-of-view (FOV) and high-resolution imaging requires microscopy modalities to have large space-bandwidth products. Lensfree on-chip microscopy decouples resolution from FOV and can achieve a space-bandwidth product greater than one billion under unit magnification using state-of-the-art opto-electronic sensor chips and pixel super-resolution techniques. However, using vertical illumination, the effective numerical aperture (NA) that can be achieved with an on-chip microscope is limited by a poor signal-to-noise ratio (SNR) at high spatial frequencies and imaging artifacts that arise as a result of the relatively narrow acceptance angles of the sensor's pixels. Here, we report, for the first time, a synthetic aperture-based on-chip microscope in which the illumination angle is scanned across the surface of a dome to increase the effective NA of the reconstructed lensfree image to 1.4, achieving e.g., ,250-nm resolution at 700-nm wavelength under unit magnification. This synthetic aperture approach not only represents the largest NA achieved to date using an on-chip microscope but also enables color imaging of connected tissue samples, such as pathology slides, by achieving robust phase recovery without the need for multi-height scanning or any prior information about the sample. To validate the effectiveness of this synthetic aperture-based, partially coherent, holographic on-chip microscope, we have successfully imaged color-stained cancer tissue slides as well as unstained Papanicolaou smears across a very large FOV of 20.5 mm 2 . This compact on-chip microscope based on a synthetic aperture approach could be useful for various applications in medicine, physical sciences and engineering that demand high-resolution wide-field imaging. Keywords: computational imaging; lensfree microscopy; on-chip microscopy; synthetic aperture INTRODUCTIONWide field-of-view (FOV) and high-resolution imaging is crucial for various applications in biomedical and physical sciences. Such tasks demand microscopes to have large space-bandwidth products (SBPs) with minimal spatial aberrations that distort the utilization of the SBP of the imaging system. Conventional lens-based digital microscopes can achieve high-resolution imaging over a large FOV using mechanical scanning stages to capture multiple images from different parts of the specimen that are digitally stitched together. This scanning approach, however, demands a relatively bulky and expensive imaging set-up. In contrast, recent advances in digital components and computational techniques have enabled powerful imaging methods,

show abstract

“…This trade-off results in time-consuming and costly mechanical scanning procedures when microscopic images across large areas are needed. In the past two decades digital holographic microscopy has become a competitive imaging technique in comparison to conventional microscopy tools since it does not necessarily require objective lenses to achieve high resolution imaging [1]- [5]. Along the same lines, our research group has recently developed a lensfree holographic microscope which decouples the lateral resolution and FOV from each other while also maintaining a compact, light-weight and cost-effective design [6]- [18].…”

Section: Introductionmentioning

confidence: 99%

Giga-pixel imaging on a chip: High numerical aperture lensfree microscopy over a wide field-of-view

Luo

Greenbaum

Ozcan

2013

2013 IEEE 10th International Symposium on Biomedical Imaging

View full text Add to dashboard Cite

We report an on-chip lensfree holographic microscope with a large field-of-view (FOV) of ~20.5 mm 2 and a high numerical aperture (NA) of ~ 0.9. By implementing a pixel super-resolution technique on a color CMOS image sensor, this computational microscope achieves a spatial resolution of ~300 nm at an illumination wavelength of 530 nm, yielding approximately one billion useful pixels in both the reconstructed amplitude and phase images. This lensfree holographic on-chip imaging technique can be used as the building block for high resolution, compact and costeffective optical microscopes for various biomedical imaging applications.

show abstract

High resolution (NA=08) in lensless in-line holographic microscopy with glass sample carriers

Cited by 42 publications

References 15 publications

Compact system design based on digital in-line holographic microscopy configuration

Compact system design based on digital in-line holographic microscopy configuration

Synthetic aperture-based on-chip microscopy

Giga-pixel imaging on a chip: High numerical aperture lensfree microscopy over a wide field-of-view

Contact Info

Product

Resources

About