High‐resolution optical coherence tomography as a non‐destructive monitoring tool for the engineering of skin equivalents

Spöler, Felix; Först, Michael; Marquardt, Yvonne; Hoeller, Daniela; Kurz, H.; Abuzahra, Faris

doi:10.1111/j.0909-752x.2006.00163.x

Cited by 36 publications

(30 citation statements)

References 22 publications

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“…The continued development of OCT technology over recent years has significantly increased its use in a variety of fields, both research and clinically driven. Although a well-established diagnostic tool in the field of ophthalmology (20,21), modern technical advances, such as an increased resolution via improved laser sources (22), enhanced sensitivity through the use of phase information (23)(24)(25), accelerated scanning speeds (26,27) and a larger field-of-view (28,29), have enabled the exploration of OCT applications in dermatology (30,31). With regards to cutaneous repair or regeneration, for example, OCT has been used in conjunction with other techniques, such as multiphoton microscopy and histopathology, to monitor over time wound healing in mice (5), wound healing in an engineered skin equivalent tissue model (32), and scar formation during skin substitute-assisted wound healing (33).…”

Section: Introductionmentioning

confidence: 99%

Optical coherence tomography angiography monitors human cutaneous wound healing over time

Deegan¹,

Men²,

Li³

et al. 2018

Quant. Imaging Med. Surg.

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Background: In vivo imaging of the complex cascade of events known to be pivotal elements in the healing of cutaneous wounds is a difficult but essential task. Current techniques are highly invasive, or lack the level of vascular and structural detail required for accurate evaluation, monitoring and treatment. We aimed to use an advanced optical coherence tomography (OCT)-based angiography (OCTA) technique for the noninvasive, high resolution imaging of cutaneous wound healing.Methods: We used a clinical prototype OCTA to image, identify and track key vascular and structural adaptations known to occur throughout the healing process. Specific vascular parameters, such as diameter and density, were measured to aid our interpretations under a spatiotemporal framework.Results: We identified multiple distinct, yet overlapping stages, hemostasis, inflammation, proliferation, and remodeling, and demonstrated the detailed vascularization and anatomical attributes underlying the multifactorial processes of dermatologic wound healing.Conclusions: OCTA provides an opportunity to both qualitatively and quantitatively assess the vascular response to acute cutaneous damage and in the future, may help to ascertain wound severity and possible healing outcomes; thus, enabling more effective treatment options.

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Section: Introductionmentioning

confidence: 99%

Optical coherence tomography angiography monitors human cutaneous wound healing over time

Deegan¹,

Men²,

Li³

et al. 2018

Quant. Imaging Med. Surg.

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“…There has been growing interest in research into EpSCs and their roles in homeostasis, wound repair and tumorigenesis. EpSCs are also becoming attractive as a therapeutic option in tissue bioengineering, gene therapy and the treatment of a variety of diseases [4][5][6][7][8][9]. Because EpSCs are important for both basic research and clinical application, establishing an approach to separate EpSC populations from the epidermis is of paramount importance.…”

Section: Introductionmentioning

confidence: 99%

Enrichment of putative human epidermal stem cells based on cell size and collagen type IV adhesiveness

Miao

Guo

et al. 2007

Cell Res

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The enrichment and identification of human epidermal stem cells (EpSCs) are of paramount importance for both basic research and clinical application. Although several approaches for the enrichment of EpSCs have been established, enriching a pure population of viable EpSCs is still a challenging task. An improved approach is worth developing to enhance the purity and viability of EpSCs. Here we report that cell size combined with collagen type IV adhesiveness can be used in an improved approach to enrich pure and viable human EpSCs. We separated the rapidly adherent keratinocytes into three populations that range in size from 5-7 μm (population A), to 7-9 μm (population B), to ≥9 μm (population C) in diameter, and found that human putative EpSCs could be further enriched in population A with the smallest size. Among the three populations, population A displayed the highest density of β1-integrin receptor, contained the highest percentage of cells in G0/G1 phase, showed the highest nucleus to cytoplasm ratio, and possessed the highest colony formation efficiency (CFE). When injected into murine blastocysts, these cells participated in multi-tissue formation. More significantly, compared with a previous approach that sorted putative EpSCs according to β1-integrin antibody staining, the viability of the EpSCs enriched by the improved approach was significantly enhanced. Our results provide a putative strategy for the enrichment of human EpSCs, and encourage further study into the role of cell size in stem cell biology.

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“…Ultrahigh resolution systems utilize lasers with large spectral bandwidths, such as Ti:sapphire femtosecond lasers, resulting in a coherence length or axial resolution <4 mm in air. 12,13 Ultrahigh resolution OCT has been used to image skin wound healing in mice 12 as well as to image skin equivalents consisting of keratinocytes and fibroblasts grown in collagen gels. 13 Morphological features, such as the dermal-epidermal junction, could be observed in both cases.…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

Imaging hydrogel implants in situ

Patterson

2011

Biomedical Hydrogels

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High‐resolution optical coherence tomography as a non‐destructive monitoring tool for the engineering of skin equivalents

Cited by 36 publications

References 22 publications

Optical coherence tomography angiography monitors human cutaneous wound healing over time

Optical coherence tomography angiography monitors human cutaneous wound healing over time

Enrichment of putative human epidermal stem cells based on cell size and collagen type IV adhesiveness

Imaging hydrogel implants in situ

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