Localized surface
plasmon resonance (LSPR) biosensors show great
potential for practical/commercial use in clinical diagnosis, home
healthcare, environmental analysis, and public healthcare. However,
two main issues, that is, low refractometric sensitivity and low reproducibility
(large-area uniformity and batch-to-batch consistency), hinder the
extensive applications of LSPR biosensors. Therefore, plasmonic nanostructures
with high sensitivity and excellent reproducibility are desirable
for preparing reliable LSPR sensors. Herein, we have fabricated plasmonic
nanopyramid arrays (NPAs) for several batches with reproducible morphology
and optical properties by elastic soft lithography and metal thermal
evaporation. NPAs of various metals (i.e., Al, Au, and Ag) were also
prepared by thermal evaporation with the according metals. The transmission
spectra of these NPAs showed several narrow LSPR peaks in the visible-infrared
wavelength region. The refractometric sensitivities of the LSPR peaks
were systematically studied, and high refractometric sensitivities
of 774.0, 472.8, and 421.0 nm/RIU were achieved on Al, Au, and Ag
NPAs, respectively. To demonstrate the potential of the NPAs for multiplex
applications, we first applied this highly sensitive Al NPA biosensor
to monitoring the process of proliferation of HeLa cancer cells, in
situ and in real time. Then, we demonstrated that the Au NPA was
able to identify the absorbed analytes on its surface through the
surface-enhanced Raman scattering spectrum. In addition, the finite
difference time domain simulations were performed to reveal the electromagnetic
field enhancement on NPAs. Because of the properties of high sensitivity
and excellent reproducibility of the metal NPA LSPR substrates, as
well as the simplicity and cost efficiency of the fabrication method,
our proposed work will accelerate the practical use of LSPR sensors.