High-resolution peptide mapping of cerebrospinal fluid: a novel concept for diagnosis and research in central nervous system diseases

Heine, Gabriele; Zucht, Hans Dieter; Schuhmann, Martín U.; Bürger, Katharina; Jürgens, Michael; Zumkeller, M.; Schneekloth, Carsten G; Hampel, Harald; Schulz‐Knappe, Peter; Selle, Hartmut

doi:10.1016/s1570-0232(02)00571-8

Cited by 35 publications

(31 citation statements)

References 30 publications

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“…Peptide mapping serves as an important tool for protein identification, for sequence determination of internal parts of polypeptide chains, for monitoring of posttranslational modification, and structure elucidation of proteins. In addition, peptide map can be obtained also as a pattern obtained by two-or multidimensional separation of peptides present in complex biological fluids, e.g., high-resolution peptide mapping of cerebrospinal fluid by LC-MS or CE-MS has been suggested as a novel concept for diagnosis and research in central nervous system disease [281]. Due to the high complexity of peptide maps, namely of large proteins, usually multidimensional separations, 2-DE, 2-DE-MS, HPLC-CZE, HPLC-MS, CZE-MS, and HPLC-CZE-MS, are necessary for complete resolution of these mixtures (see Section 5.7).…”

Section: Peptide Mappingmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Peptide Mappingmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…However, the number of studies that are focusing on natural occurring peptides has been limited to date, probably due to sensitivity issues. The required volume of CSF for these analyses was at least 0.5 ml per analysis [46,47]. This is a large volume in comparison with the 3-5 ml of CSF that is routinely taken from patients for diagnostic purposes.…”

Section: Profiling Intact Proteins and Naturally Occurring Peptidesmentioning

confidence: 99%

Peptide profiling of cerebrospinal fluid by mass spectrometry

Dekker

Burgers

Kros

et al. 2006

Expert Review of Proteomics

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The search for biomarkers is driven by the increasing clinical importance of early diagnosis. Reliable biomarkers can also assist in directing therapy, monitoring disease activity and the efficacy of treatment. In addition, the discovery of novel biomarkers might provide clues to the pathogenesis of a disease. The dynamic range of protein concentrations in body fluids exceeds 10 orders of magnitude. These huge differences in concentrations complicate the detection of proteins with low expression levels. Since all classical biomarkers have low expression levels (e.g., prostate-specific antigen: 2-4 microg/l; and CA125: 20-35 U/ml), new developments with respect to identification and validation techniques of the low-abundance proteins are required. This review will discuss the current status of profiling cerebrospinal fluid using mass spectrometry-based techniques, and new developments in this area.

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“…Peptide mapping serves as an important tool for protein identification, for sequence determination of internal parts of polypeptide chains, for monitoring of post-translational modification and structure elucidation of proteins. In addition, a peptide map can be obtained also as a pattern obtained by one-or multidimensional separation of peptides present in complex biological fluids, e.g., high-resolution peptide mapping of cerebrospinal fluid by LC-MS or CE-MS has been suggested as a novel concept for diagnosis and research in a central nervous system disease [245]. Due to the high complexity of peptide maps, namely of large proteins, usually multidimensional separations, 2-DE, 2-DE-MS, HPLC-CZE, HPLC-MS, CZE-MS, HPLC-CZE-MS, are necessary for complete resolution of these mixtures [10,141].…”

Section: Peptide Mappingmentioning

confidence: 99%

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Kasicka

2003

Electrophoresis

106

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An overview of the recent developments in the applications of high-performance capillary electromigration methods, namely zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides is presented. New approaches to the theoretical description and experimental verification of the electromigration behavior of peptides and the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample treatment, and suppression of adsorption, are discussed, and new developments in individual separation modes and new designs of detection systems applied to peptide separations are shown. Several types of applications of capillary electromigration methods to peptide analysis are presented: quality control and purity tests, determination in biomatrices, monitoring of physical and chemical changes and enzymatic conversions, amino acid and sequence analysis, and peptide mapping. The examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

show abstract

High-resolution peptide mapping of cerebrospinal fluid: a novel concept for diagnosis and research in central nervous system diseases

Cited by 35 publications

References 30 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Peptide profiling of cerebrospinal fluid by mass spectrometry

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

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