High-resolution proton nuclear magnetic resonance spectroscopy of ovarian cyst fluid

Preliminary observations and clinical value of N-acetyl resonances in ovarian tumours using in-vivo proton MR spectroscopy at 3T

“…The metabolite peak at 2 ppm was detected in various mucinous and nonmucinous gynaecologic lesions by MR spectroscopy [21][22][23]. Boss et al detected this peak in five of 11 serous cystadenomas by ex-vivo MR spectroscopy and assigned this peak as NAA, however, they also stated that NAA is usually present in living neurons and may be undetectable in other cell types and further investigation is needed [21]. Hascalik et al detected this peak in an ovarian mucinous cystadenoma by in-vivo MR spectroscopy and considered this peak as NAA [22].…”

“…Lip: Lipid peak centered at 1.3 ppm mucinous compounds which was probably related to glycoproteins in mucin [8][9][10]. The metabolite peak at 2 ppm was detected in various mucinous and nonmucinous gynaecologic lesions by MR spectroscopy [21][22][23]. Boss et al detected this peak in five of 11 serous cystadenomas by ex-vivo MR spectroscopy and assigned this peak as NAA, however, they also stated that NAA is usually present in living neurons and may be undetectable in other cell types and further investigation is needed [21].…”

“…However, low peak at 2 ppm was also detected in various non-mucinous tumours. This result suggests that the peak at 2 ppm may also contain some metabolites other than N-acetyl mucinous compounds, which is compatible with the result of Boss et al [21]. In vitro chromatography or high-resolution nuclear magnetic resonance (NMR) may be needed in the future to explore the chemical origin of this peak observed in non-mucinous tumours.…”

Metabolomics in cell culture—A strategy to study crucial metabolic pathways in cancer development and the response to treatment

“…Therefore, there is increased interest in the discovery of novel diagnostic biomarkers [42,113] and in the identification of factors involved in cancer invasiveness and growth [114,115]. The implementation of metabolomics to study human biofluids and ovarian cyst fluids uncovers a decreased level of valine, alanine and phospholipids, and an increased level of ketone bodies in the serum of patients with ovarian carcinoma [116], together with an increase in isoleucine, valine, lysine, lactate and choline concentrations in malignant cyst fluids [117]. Furthermore, an ''abnormal'' phosphatidylcholine concentration has been recognised as a feasible biomarker for progression of ovarian carcinoma with potential application for diagnosis and improvement of treatment efficiency [118].…”

Padé optimization of noise-corrupted magnetic resonance spectroscopic time signals from fibroadenoma of the breast

“…We first applied the FPT to noiseless time signals associated with MRS data for benign and cancerous ovarian cyst fluid from Ref. [86]. The spectral parameters for all twelve genuine metabolites, including those that were quite closely-lying, were accurately reconstructed by the FPT using very small fractions (N /16 = 64) of the time signal of full length N = 1024 [72,87,88], whereas the FFT produced completely uninterpretable spectra at these short signal lengths [72,87,88].…”