High-Resolution Screening of Amino-Acidurias

Rapid Technique for the Presymptomatic Detection of Inborn Errors of Amino Acid Metabolism

“…Med., 1965) seems to have been wasted as only one or two pieces of information have been obtained from each specimen. More generalised screening procedures for amino-acidurias have been undertaken (Turner and Juster, 1964;Sackett, 1964;Samuels, 1964); Samuels has developed the method of combined high-voltage electrophoresis and chromatograpny to handle up to 100 specimens a day. However, the capital outlay for high-voltage electrophoresis equipment may be beyond the means of many routine laboratories.…”

Electrophoresis

“…Variant techniques for Immunoelectrophoresis have been given descriptive names: stereoimmunoelectrophoresis (1763), immunoosmophoresis (258,259), and antigen-antibody crossed electrophoresis (1275). There are many procedures for separating and measuring amino acids (89, 298,439,671,1020,1076,1926,1943,2366), some of which combine electrophoresis and chromatography (216,761,1021,1655,1706). Physiological cations can cause false ninhydrin reactions in electrophoregrams of amino acids (820).…”

Biochemical Analysis

“…High voltage electrophoresis of amino acids was reviewed (46). The method was applied to the determination of amino acids in serum (64,SOI) and in urine (421,426) and to the identification of biogenic amines in feces of cattle (403). A micromethod of determining 0.01 micromole or less of amino acids by high voltage electrophoresis was devised (47); the accuracy was stated asab out 5%.…”