High-resolution three-dimensional probes of biomaterials and their interfaces

Grandfield, Kathryn; Palmquist, Anders; Engqvist, Håkan

doi:10.1098/rsta.2011.0253

Cited by 23 publications

(23 citation statements)

References 78 publications

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“…Conventional bright-field TEM images and high-angle annular dark-field (HAADF) scanning TEM (STEM) images with compositional contrast [22] were recorded. High-resolution TEM (HRTEM) was utilized to visualize lattice fringes representative of crystalline structure.…”

Section: Methodsmentioning

confidence: 99%

The plastic nature of the human bone–periodontal ligament–tooth fibrous joint

Kurylo

Grandfield

et al. 2013

Bone

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This study investigates bony protrusions within a narrowed periodontal ligament space (PDL-space) of a human bone-PDL-tooth fibrous joint by mapping structural, biochemical, and mechanical heterogeneity. Higher resolution structural characterization was achieved via complementary atomic force microscopy (AFM), nano transmission X-ray microscopy (nano-TXM), and micro tomography (Micro XCT™). Structural heterogeneity was correlated to biochemical and elemental composition, illustrated via histochemistry and microprobe X-ray fluorescence analysis (μ-XRF), and mechanical heterogeneity evaluated by AFM-based nanoindentation. Results demonstrated that the narrowed PDL-space was due to invasion of bundle bone (BB) into PDL-space. Protruded BB had a wider range with higher elastic modulus values (2-8 GPa) compared to lamellar bone (0.8-6 GPa), and increased quantities of Ca, P and Zn as revealed by μ-XRF. Interestingly, the hygroscopic 10-30 μm interface between protruded BB and lamellar bone exhibited higher X-ray attenuation similar to cement lines and lamellae within bone. Localization of the small leucine rich proteoglycan biglycan (BGN) responsible for mineralization was observed at the PDL-bone interface and around the osteocyte lacunae. Based on these results, it can be argued that the LB-BB interface was the original site of PDL attachment, and that the genesis of protruded BB identified as protrusions occurred as a result of shift in strain. We emphasize the importance of bony protrusions within the context of organ function and that additional study is warranted.

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Section: Methodsmentioning

confidence: 99%

The plastic nature of the human bone–periodontal ligament–tooth fibrous joint

Kurylo

Grandfield

et al. 2013

Bone

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“…Given the resolution level, we believe that ACOM-TEM could be advantageously exploited to analyze the interface layer (typically < 1 µm) between biomaterials and bone formed at the surface of implants, a critical aspect of osseointegration [41,42]. TEM was widely used to investigate the tissue structure at this interface [43,44], but the collective mineral nanocrystals structure and organization was never analyzed. Similarly, in the biomedical field, severe pathological perturbations of mineral nanocrystals have been reported in many bone diseases, e.g.…”

Section: Discussionmentioning

confidence: 99%

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

et al. 2018

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To cite this version:M. Verezhak, E.F. Rauch, M. Veron, C. Lancelon-Pin, J.-L. Putaux, et al.. Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level. Acta Biomaterialia, Elsevier, 2018Elsevier, , 10.1016Elsevier, /j.actbio.2018 Online version: https://www.sciencedirect.com/science/article/pii/S1742706118301946 Abstract: The nanoscale characteristics of the mineral phase in bone tissue such as nanocrystal size, organization, structure and composition have been identified as potential markers of bone quality. However, such characterization remains challenging since it requires combining structural analysis and imaging modalities with nanoscale precision. In this paper, we report the first application of automated crystal orientation mapping using transmission electron microscopy (ACOM-TEM) to the structural analysis of bone mineral at the individual nanocrystal level. By controlling the nanocrystal growth of a cortical bovine bone model artificially heated up to 1000 ºC, we highlight the potential of this technique. We thus show that the combination of sample mapping by scanning and the crystallographic information derived from the collected electron diffraction patterns provides a more rigorous analysis of the mineral nanostructure than standard TEM. In particular, we demonstrate that nanocrystal orientation maps provide valuable information for dimensional analysis. Furthermore, we show that ACOM-TEM has sufficient sensitivity to distinguish between phases with close crystal structures and we address unresolved questions regarding the existence of a hexagonal to monoclinic phase transition induced by heating. This first study therefore opens new perspectives in bone characterization at the nanoscale, a daunting challenge in the biomedical and archaeological fields, which could also prove particularly useful to study the mineral characteristics of tissue grown at the interface with biomaterials implants.

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“…three-dimensional analysis of tissue-biomaterial interfaces and for resolving the ultrastructure of actomyosin linkages and intracellular Ca 2þ stores within muscle cells [30,98,99].…”

Section: Electron Microscopymentioning

confidence: 99%

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

Vielreicher

Schürmann

Detsch

et al. 2013

J. R. Soc. Interface.

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This review focuses on modern nonlinear optical microscopy (NLOM) methods that are increasingly being used in the field of tissue engineering (TE) to image tissue non-invasively and without labelling in depths unreached by conventional microscopy techniques. With NLOM techniques, biomaterial matrices, cultured cells and their produced extracellular matrix may be visualized with high resolution. After introducing classical imaging methodologies such as µCT, MRI, optical coherence tomography, electron microscopy and conventional microscopy two-photon fluorescence (2-PF) and second harmonic generation (SHG) imaging are described in detail (principle, power, limitations) together with their most widely used TE applications. Besides our own cell encapsulation, cell printing and collagen scaffolding systems and their NLOM imaging the most current research articles will be reviewed. These cover imaging of autofluorescence and fluorescence-labelled tissue and biomaterial structures, SHG-based quantitative morphometry of collagen I and other proteins, imaging of vascularization and online monitoring techniques in TE. Finally, some insight is given into state-of-the-art three-photon-based imaging methods (e.g. coherent anti-Stokes Raman scattering, third harmonic generation). This review provides an overview of the powerful and constantly evolving field of multiphoton microscopy, which is a powerful and indispensable tool for the development of artificial tissues in regenerative medicine and which is likely to gain importance also as a means for general diagnostic medical imaging.

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High-resolution three-dimensional probes of biomaterials and their interfaces

Cited by 23 publications

References 78 publications

The plastic nature of the human bone–periodontal ligament–tooth fibrous joint

The plastic nature of the human bone–periodontal ligament–tooth fibrous joint

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

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