High-Reynolds Microfluidic Sorting of Large Yeast Populations

Keinan, Eliezer; Abraham, Ayelet Chen; Cohen, Anna; Alexandrov, Alexander I.; Mintz, Reshef; Cohen, Merav; Reichmann, Dana; Kaganovich, Daniel; Nahmias, Yaakov

doi:10.1038/s41598-018-31726-6

Cited by 10 publications

(10 citation statements)

References 53 publications

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“…One strategy of generating precise high shear stress in a microchannel is to use centrifugal force. [ 38–40 ] Centrifugal force can be easily applied to a microfluidic channel by simply placing the microfluidic channel on a spin coater and spinning the microfluidic channel at a set rotation speed. The degree of centrifugal force can also be readily controlled by adjusting the rotation speed.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Targeted Material Binding Microorganisms Using a Centrifugal Microfluidic Platform

Han

Sarkes

Jahnke

et al. 2021

Adv Materials Technologies

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Discovery of unique peptides that specifically bind to target materials of interest is commonly achieved through biopanning‐based screening of cell libraries having high diversity. However, conventional methods have limitations in the generation of controllable shear stress applied in order to effectively remove weak and unbound cells from a target material. Here, a centrifugal force‐based microfluidic biopanning platform is presented. Using this microfluidic platform, a wide range of shear stress conditions can be generated by simply adjusting the rotation speed of the platform, which provides a precisely controllable wash step. The enhanced circularly permuted outer membrane protein X (eCPX) 3.0 bacterial peptide display library (≈1011 number of cells) is screened, and many isolates that show strong affinity towards gold only, ITO only, or both are successfully obtained through a multiround iterative sorting process. Amino acid contents of the resulting isolates are analyzed to provide a better understanding of the distinctive attributes of these peptides and their specific material‐binding capabilities. This platform is simple and easy to use and can be readily applied to screen microbial libraries against virtually any material surface of interest, which can greatly accelerate the discovery of peptide‐driven biomaterials and the development of hybrid organic–inorganic materials.

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Section: Introductionmentioning

confidence: 99%

Discovery of Targeted Material Binding Microorganisms Using a Centrifugal Microfluidic Platform

Han

Sarkes

Jahnke

et al. 2021

Adv Materials Technologies

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“…In the literature review, various powerful microfluidic-cell-separation solutions [ 4 , 18 , 29 ] were presented. Their channel geometry and cross-sectional parameters mainly determine the possible cell sizes and morphologies to be separated and focused.…”

Section: Discussionmentioning

confidence: 99%

“…Eliezer Kainan et al [ 29 ] performed inertial focusing and sorting of Saccharomyces cereviae in a curved channel at higher Reynolds numbers ( Re = 215, 1.5 mL/min) to segregate yeast based on age-related cell size. The degree of budding is a good indicator of cell aging.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels

Bányai

Farkas

Jankovics

et al. 2023

Sensors

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The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (Ø = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 µm) at different flow rates (0.5, 1, 2 µL/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped Escherichia coli (E. coli), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, Saccharomyces cerevisiae, and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel.

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“…Keinan et al 28 combined this mechanism of inertial focusing with peripheral Dean vortices to isolate 10 7 yeast cells/min from yeast suspensions based on size differences of 1.5 μm. The platform provided the ability to isolate large quantities of microorganisms for proteomic and metabolomic analyses.…”

Section: ■ Cell Separation Methodsmentioning

confidence: 99%

“…Even though CTCs hold a strong presence with respect to isolation and enrichment platforms, there are other type of cells for which novel platforms were demonstrated. For example, technologies were presented for the screening and analysis of bacterial cells, − yeast cells, , immune cells, , or characterization of red blood cells. , This review will cover methods for cell separation and sorting based on numerous phenomena, such as acoustophoresis, affinity, dielectrophoresis and inertial forces, size and elasticity, deterministic lateral displacement, flow fractionation, and density gradient centrifugation. We cover in this review magnetic, impedance, and fluorescence-activated cell sorters as well as droplet-based methods.…”

Section: Need For Cell Separations and Sorting Technologiesmentioning

confidence: 99%

Cell Separations and Sorting

2019

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The development of new methods and technologies for cell separation, sorting, selection, isolation, or enrichment (many times the aforementioned terms are used interchangeably) are becoming more imperative due in part to the wealth of research aimed at addressing issues for the analysis of liquid biopsy targets, such as rare cells, to realize the concept of "precision medicine". Precision medicine is defined as "treatments targeted to the needs of individual patients on the basis of genetic, biomarker, phenotypic, or psychosocial characteristics that distinguish a patient from others with similar clinical presentations." 1 Before full implementation of precision medicine in a hospital or clinical setting, technological discoveries must be realized to assist in the analysis of disease-associated cells enriched from complex samples.Liquid biopsy markers and the cargo that they carry have been shown to be an attractive source of information that can be used to facilitate precise decisions for disease management. These biomarkers include, but are not limited to, rare cells such as circulating tumor cells (CTCs) or cancer stem cells (CSCs), immune cells (i.e., CD8+ T-cells), bacterial and viral cells, cell-free molecules such as cell free DNA (cfDNA), and extracellular vesicles (EVs). Liquid biopsies are attractive because of the minimally invasive nature of the sampling method (i.e., collection of peripheral blood, urine, saliva) to secure relevant biomarkers. These biomarkers can enable precision medicine decisions on managing a variety of diseases, including oncology and nononcology-related diseases. 2,3 Liquid biopsies *

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High-Reynolds Microfluidic Sorting of Large Yeast Populations

Cited by 10 publications

References 53 publications

Discovery of Targeted Material Binding Microorganisms Using a Centrifugal Microfluidic Platform

Discovery of Targeted Material Binding Microorganisms Using a Centrifugal Microfluidic Platform

Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels

Cell Separations and Sorting

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