1997
DOI: 10.1080/15216549700202321
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High salt and solvent induced Z‐conformation in native calf thymus DNA

Abstract: The Z‐DNA forming potentiality of native calf thymus DNA using spectrophotometric measurements and diethyl pyrocarbonate reactivity have been investigated. The [image omitted] value was evaluated from absorbance ratio for B‐ and Z‐DNA and compared with the values obtained for native DNA. The results suggest that native DNA undergoes B‐ to Z‐/Z‐analogous transition to an extent of 20 per cent under constraint conditions. Possible Z‐DNA formation was also characterized by hyper‐reactivity of native DNA towards d… Show more

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Cited by 6 publications
(9 citation statements)
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“…Additionally, Z-DNA binding proteins, although rare, are involved in gene regulation (Oh et al, 2002), viral pathogenesis (e.g., E3L) (Kim et al, 2003;Kwon and Rich, 2005), innate immune sensing (e.g., ZBP1) (Kuriakose and Kanneganti, 2018;Newton et al, 2016), DNA recognition (e.g., ADAR-1) (Kim et al, 2000), and inflammation (Szczesny et al, 2018). There is also ample experimental evidence of Z-DNA formation in the presence of high ionic strength (Ali and Ali, 1997;Peck et al, 1982), Z-DNA binding proteins (Bae et al, 2011), negative supercoiling (Nordheim et al, 1982;Nordheim and Rich, 1983;Wittig et al, 1991), as well as induction of Z-DNA via nucleotide modification (e.g., methylation and or bromination) that can reduce the high energy activation barrier (Temiz et al, 2012). Although there are Z-prone DNA sequences (e.g., alternating dGdC) (Mo ¨ller et al, 1982), all sequences of DNA are capable of conversion to the Z-form (Kypr et al, 2009).…”
Section: Introductionmentioning
confidence: 99%
“…Additionally, Z-DNA binding proteins, although rare, are involved in gene regulation (Oh et al, 2002), viral pathogenesis (e.g., E3L) (Kim et al, 2003;Kwon and Rich, 2005), innate immune sensing (e.g., ZBP1) (Kuriakose and Kanneganti, 2018;Newton et al, 2016), DNA recognition (e.g., ADAR-1) (Kim et al, 2000), and inflammation (Szczesny et al, 2018). There is also ample experimental evidence of Z-DNA formation in the presence of high ionic strength (Ali and Ali, 1997;Peck et al, 1982), Z-DNA binding proteins (Bae et al, 2011), negative supercoiling (Nordheim et al, 1982;Nordheim and Rich, 1983;Wittig et al, 1991), as well as induction of Z-DNA via nucleotide modification (e.g., methylation and or bromination) that can reduce the high energy activation barrier (Temiz et al, 2012). Although there are Z-prone DNA sequences (e.g., alternating dGdC) (Mo ¨ller et al, 1982), all sequences of DNA are capable of conversion to the Z-form (Kypr et al, 2009).…”
Section: Introductionmentioning
confidence: 99%
“…The conformation of double‐helical nucleic acids depends largely on factors like the sequence, solvent, electrolytes, and their concentrations, pH, temperature, and binding to proteins . The role of DNA in biological processes depends on its conformational properties.…”
Section: Introductionmentioning
confidence: 99%
“…Nucleic acids can adopt different conformations in solution depending on the base composition (Hunter, 1993) and the environment (for example pH and temperature, Kumar and Maiti, 1994), including the nature of the solvent (Fang et al, 1999), the counterions (Minasov et al, 1999), their concentration (Ali and Ali, 1997), and interactions with proteins (Jones et al, 1999), or small molecules (Reinert, 1999). Even a given sequence of DNA or RNA can exhibit multiple conformations (Kielkopf et al, 2000).…”
Section: Introductionmentioning
confidence: 99%