High salt‐treatment‐induced Na⁺ extrusion and low salt‐treatment‐induced Na⁺ accumulation in suspension‐cultured cells of the mangrove plant, Bruguiera sexangula

Stimulatory effects of sea salts on cell growth in liquid culture of Avicenniaceae mangrove

“…Callus induction in A. alba was observed in mAA medium in the presence of both 2,4-D and BA at the concentration of 0.1-10 mM, but not in MS. Though the AA basal medium was more profitable than MS for callus-induction in solid culture (Mimura et al 1997) and cell suspension culture of B. sexangula (Kura-Hotta et al 2001), mAA was better than MS and AA when the 24-well culture plate method was used for suspension culture of A. alba (data not shown). Since the only difference between mAA and AA is the glycine content, and the total N content did not differ substantially, amino acid metabolism might be important for the suspension culture of A. alba.…”

“…However, mangrove plants are very recalcitrant in cell culture with the exception of a few species in the families Rhizophoraceae (Bruguiera sexangula) (Mimura et al 1997, Kura-Hotta et al 2001 and Sonneratiaceae (Sonneratia alba and S. caseolaris) (Kawana et al 2007, Yamamoto et al 2009) which are amenable to suspension culture studies. The effects of sea salts on the growth of mangrove have been investigated using suspension cultures of B. sexangula from leaves and of S. alba from cotyledons.…”

“…However, all four salts were inhibitory at high concentrations. In B. sexangula, callus induction in solid culture in leaf tissue was less tolerant to NaCl than that in viviparous seedlings (Mimura et al 1997), and it is important to note that the above-mentioned cell suspension culture of B. sexangula was obtained from leaf-derived calluses (Kura-Hotta et al 2001). In B. sexangula, cell suspension culture has been used for biochemical (SuzukiYamamoto et al 2006) and molecular biological studies alba after 40 days of culture at 30°C.…”

Salt-induced respiration in Bruguiera cylindrica — role in salt transport and protection against oxidative damage

“…Bruguiera species are known to accumulate salt to levels higher than that of sea water (Takemura et al, 2000;Kura-Hotta et al, 2001). Salt is thought to act as a cheap and easily available osmoticum, which maintains an inward flow of water into the plant (Shabala, 2000;Pardo and Quintero, 2002).…”

Effects of Sea Salts on Induction of Cell Proliferation in Liquid Cultures of Mangrove Plants, Sonneratia caseolaris and S. alba

“…The development of in vitro culture systems is important for complementation of whole plant studies. In 2001, the successful establishment of a suspension culture from leaf explants of Bruguiera sexangula (Rhisophoraceae) was reported [7,8]. Protoplast fusion studies [9] and molecular biological work on salt-tolerance related genes has since been performed [10].…”