High-Sensitivity Chemiluminescence Immunoassays for Detection of Growth Hormone Doping in Sports

Bidlingmaier, Martin; Suhr, Jennifer; Ernst, Andrea; Wu, Zida; Keller, Alexandra; Strasburger, Christian J.; Bergmann, Andreas

doi:10.1373/clinchem.2008.112458

Cited by 123 publications

(99 citation statements)

References 29 publications

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“…The GH secretion was higher in females following AMI, which resembles the findings using fasting GH levels in healthy subjects within the general population (5,6). We also demonstrated a weak correlation to troponin levels, which suggests some association with infarct size.…”

Section: Discussionsupporting

confidence: 81%

Growth hormone for risk stratification and effects of therapy in acute myocardial infarction

Bhandari

Sandhu

et al. 2015

Biomarkers

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ContextExcess growth hormone is associated with early mortality. In healthy populations, high GH levels was associated with increased mortality. ObjectivesWe assessed the association of Growth Hormone (GH) with prognosis after acute myocardial infarction(AMI), and the effects of secondary prevention therapies on outcome stratified by GH levels. MethodsGH was measured using a high-sensitivity assay in 953 (687 male, mean age 66.1 ± 12.8 years) AMI patients. The primary outcome was major adverse events (MACE, a composite of death, re-AMI, heart failure (HF) hospitalization). respectively) with significant interactions with beta blocker therapy (p=0.047) and ACE/ARB therapy (p=0.016). Prescription of beta blocker therapy and ACE/ARB was most effective at reducing MACE in those patients in the top GH tertile (p<0.0005). Results During ConclusionsGH levels post-AMI are prognostic for MACE and may indicate those patients who benefit from beta blocker and ACE/ARB therapy.

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Section: Discussionsupporting

confidence: 81%

Growth hormone for risk stratification and effects of therapy in acute myocardial infarction

Bhandari

Sandhu

et al. 2015

Biomarkers

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“…Washing procedure was repeated before luminometer reading. [12] hGH: Biomarkers IGF-I: Immunotech kit (Beckman-Coulter, Czech Republic) was used according to the manufacturer's instructions. Briefly, 25 μL of serum samples and controls were incubated with 500 μL of dissociation buffer (30-90 minutes, RT).…”

Section: Erythropoiesis Stimulating Agents (Esas)mentioning

confidence: 99%

“…The XXXI Summer Olympic Games (August [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]2016) and the XV Paralympic Games (September 7-18, 2016) were held in Rio de Janeiro, marking the first time that these events were organised in South America. Doping control analyses have been conducted in Brazil since 1989, were incorporated into the International Olympic Committee (IOC) accreditation in 2002, and were absorbed by the World Anti-Doping Agency (WADA) accreditation system in 2005.…”

Section: Introduction and Facility Descriptionmentioning

confidence: 99%

Doping control analysis at the Rio 2016 Olympic and Paralympic Games

Pereira

Sardela

Padilha

et al. 2017

Drug Testing and Analysis

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This paper summarises the results obtained from the doping control analyses performed during the Summer XXXI Olympic Games (August 3-21, 2016) and the XV Paralympic Games (September 7-18, 2016). The analyses of all doping control samples were performed at the Brazilian Doping Control Laboratory (LBCD), a World Anti-Doping Agency (WADA)-accredited laboratory located in Rio de Janeiro, Brazil. A new facility at Rio de Janeiro Federal University (UFRJ) was built and fully operated by over 700 professionals, including Brazilian and international scientists, administrative staff, and volunteers. For the Olympic Games, 4913 samples were analysed. In 29 specimens, the presence of a prohibited substance was confirmed, resulting in adverse analytical findings (AAFs). For the Paralympic Games, 1687 samples were analysed, 12 of which were reported as AAFs. For both events, 82.8% of the samples were urine, and 17.2% were blood samples. In total, more than 31 000 analytical procedures were conducted. New WADA technical documents were fully implemented; consequently, state-of-the-art analytical toxicology instrumentation and strategies were applied during the Games, including different types of mass spectrometry (MS) analysers, peptide, and protein detection strategies, endogenous steroid profile measurements, and blood analysis. This enormous investment yielded one of the largest Olympic legacies in Brazil and South America.

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“…The Immulite immunoassay as described above was conducted in George Mason University, USA and a recently developed research grade immunoassay validated for serum samples [16] was performed in IMIM Hospital del Mar, Spain. Four urine proficiency samples spiked with recombinant hGH were prepared and incubated with 1 mL of poly(NIPAm/RBB) nanoparticles.…”

Section: Inter-laboratory Proficiency Assessmentmentioning

confidence: 99%

Application of Analyte Harvesting Nanoparticle Technology to the Measurement of Urinary HGH in Healthy Individuals

Luchini¹

2012

J Sports Med Doping Stud

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Urine represents a valuable biofluid for noninvasive measurement of Human Growth Hormone (HGH) secretion. Unfortunately, currently available commercial HGH immunoassays do not achieve the sensitivity needed for urinary HGH measurement in the low picogram per milliliter range, the expected normal concentration range of HGH in urine.A nanotechnology based sample preprocessing step was used to extract and concentrate HGH in urine so that urinary HGH could be measured with a clinical grade standard immunoassay designed for serum (Immulite 1000, Siemens). We applied the nanoparticle enhanced immunoassay to evaluate the baseline value of urinary HGH in a population of healthy young adults (age 18-30, N=33, median 21, M: F=39%:61%, with no reported medical therapies).Nanoparticle sample preprocessing effectively improved the lower limit of detection of the Immulite HGH assay by more than 50 fold, shifting the linear range of the assay to encompass the expected value of urinary HGH. The full process between run and within run CV% was 7.9 and 9.0%, respectively. On 33 healthy volunteers, the 95% reference values for hGH in spot urine normalized to specific gravity were 0.64 -16.85 pg/mL (0.05-5.82 ng/g creatinine).Nanoparticle preprocessing constitutes a reliable means of measuring urinary HGH with a clinical grade immunoassay, now establishing a normal baseline value for HGH in urine. Nanoparticles can be used to study the kinetics of HGH excretion in urine, and the factors that influence urinary HGH secretion and HGH isoform proportions.

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High-Sensitivity Chemiluminescence Immunoassays for Detection of Growth Hormone Doping in Sports

Cited by 123 publications

References 29 publications

Growth hormone for risk stratification and effects of therapy in acute myocardial infarction

Growth hormone for risk stratification and effects of therapy in acute myocardial infarction

Doping control analysis at the Rio 2016 Olympic and Paralympic Games

Application of Analyte Harvesting Nanoparticle Technology to the Measurement of Urinary HGH in Healthy Individuals

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