High sequence conservation among Odotoglossum ringspot virus isolates from orchids

Abstract

The variability in the nucleotide (nt) and amino acid (aa) sequences of the coat protein (CP) of Odontoglossum ringspot virus (ORSV), which naturally infects orchids worldwide, was investigated. The CP genes of 48 ORSV isolates originating from different locations in Korea were amplified using RT-PCR and sequenced. The encoded CP consists of 158 aa. The CP sequences of the Korean isolates were compared at the nt and aa levels with those of the previously published ORSV isolates originating from different countries. The Korean isolates share 94.8-100% and 92.4-100% CP identity to ORSV isolates from other countries at the nt and aa levels, respectively. No particular region of variability could be found in either sequence of the viruses. In the deduced aa sequence, the N-terminal region was more conserved than the C-terminal region in ORSV. The phylogenetic tree analysis and recombination analysis revealed that there was no distinct grouping between geographic locations and sequence identity, and nor distinct intra-specific recombination events among ORSV isolates.

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

“…Our previous results also showed that a number of ORSV isolates caused severe damage in orchid production [21]. Among the larger population of samples showing positive reactions, the nt sequences of the CP genes of 55 Korean isolates were further determined in this study.…”

Genetic Diversities of Cymbidium mosaic virus and Odontoglossum ringspot virus Isolates Based on the Coat Protein Genes from Orchids in Guangdong Province, China

“…; Yoon et al. ). A possible reason was that the genetic functional constraints may limit variability (Alonso et al.…”

“…; Yoon et al. , ), but little is known about ORSV and CymMV isolates in Guangdong Province, China. In this study, the coat protein (CP) gene sequences of CymMV and ORSV isolates from different orchid genera in Guangdong were detected and determined.…”

Evolutionary Rates and Phylogeographical Analysis of Odontoglossum Ringspot Virus Based on the 166 Coat Protein Gene Sequences

“…The only effective control of ORSV is through the use of host plant genetic resistance, either conventional or transgenic (Ajjikuttira et al, 2002; Yoon et al, 2011). Hence, it is very important to understand the molecular variability, divergence, and migration of ORSV population in each region.…”

“…Earlier studies reported the genetic variability of ORSV isolates from Korea (Ajjikuttira et al, 2002; Yoon et al, 2011) and China (Rao et al, 2015). These showed high sequence conservation in CP gene.…”