High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

Vilela, Felipe Pinheiro; Rodrigues, Dália dos Prazeres; Costa, Renata Garcia; Tiba‐Casas, Monique Ribeiro; Falcão, Juliana Pfrimer; Campioni, Fábio

doi:10.1007/s42770-019-00156-5

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…This ST affects both humans and animals, including cattle, and has been reported to show an MDR phenotype (52)(53)(54). In agreement with other reports, S. Dublin ST10 was isolated from 5 different farms, and S. Anatum ST64 was isolated from 2 farms (55)(56)(57)(58)(59)(60).…”

Section: Discussionsupporting

confidence: 88%

Phenotypic and genotypic survey of antibiotic resistance in Salmonella enterica isolates from dairy farms in Uruguay

Casaux

D’Alessandro²,

Vignoli³

et al. 2023

Front. Vet. Sci.

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Salmonella enterica is an important zoonotic pathogen that is frequently identified in dairy farming systems. An increase in antibiotic resistance has led to inadequate results of treatments, with impacts on animal and human health. Here, the phenotypic and genotypic susceptibility patterns of Salmonella isolates from dairy cattle and dairy farm environments were evaluated and compared. A collection of 75 S. enterica isolates were evaluated, and their phenotypic susceptibility was determined. For genotypic characterization, the whole genomes of the isolates were sequenced, and geno-serotypes, sequence types (STs) and core-genome-sequence types were determined using the EnteroBase pipeline. To characterize antibiotic resistance genes and gene mutations, tools from the Center for Genomic Epidemiology were used. Salmonella Dublin (SDu), S. Typhimurium (STy), S. Anatum (SAn), S. Newport (SNe), S. Agona (Sag), S. Montevideo (SMo) and IIIb 61:i:z53 were included in the collection. A single sequence type was detected per serovar. Phenotypic non-susceptibility to streptomycin and tetracycline was very frequent in the collection, and high non-susceptibility to ciprofloxacin was also observed. Multidrug resistance (MDR) was observed in 42 isolates (56.0%), with SAn and STy presenting higher MDR than the other serovars, showing non-susceptibility to up to 6 groups of antibiotics. Genomic analysis revealed the presence of 21 genes associated with antimicrobial resistance (AMR) in Salmonella isolates. More than 60% of the isolates carried some gene associated with resistance to aminoglycosides and tetracyclines. Only one gene associated with beta-lactam resistance was found, in seven isolates. Two different mutations were identified, parC_T57S and acrB_R717Q, which confer resistance to quinolones and azithromycin, respectively. The accuracy of predicting antimicrobial resistance phenotypes based on AMR genotypes was 83.7%. The genomic approach does not replace the phenotypic assay but offers valuable information for the survey of circulating antimicrobial resistance. This work represents one of the first studies evaluating phenotypic and genotypic AMR in Salmonella from dairy cattle in South America.

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Section: Discussionsupporting

confidence: 88%

Phenotypic and genotypic survey of antibiotic resistance in Salmonella enterica isolates from dairy farms in Uruguay

Casaux

D’Alessandro²,

Vignoli³

et al. 2023

Front. Vet. Sci.

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“…CRISPR-MVLST has shown a high discriminatory ability (DI = 0.980) in typing S . Dublin recovered from humans and animals ( 41 ). CT identified 76 types with a discriminatory power of 97.6% among 180 clinical Salmonella strains isolated during 2017–2018 ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella

et al. 2022

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In public health field, high-resolution and efficient typing of the bacterial pathogen is essential, considering source-tracking and risk assessment are fundamental issues. Currently, there are no recommendations for applying molecular characterization methods for Salmonella along the food chain, and a systematic in-field investigation comparing subtyping methods in the context of routine surveillance was partially addressed.

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“…There also seems to be no association between phylogeny and persistence in S . Dublin [ 130 , 131 ]. Historically, S. Pullorum strains have existed in four major lineages [ 132 ] with two currently predominating [ 133 ] and with new CRISPR types appearing since 2001, this suggests continued evolution.…”

Section: Is There Anything Unique To These Serovars That Predisposes Them To Persistence?mentioning

confidence: 99%

Revisiting Persistent Salmonella Infection and the Carrier State: What Do We Know?

Foster¹,

Tang

Berchieri

et al. 2021

Pathogens

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One characteristic of the few Salmonella enterica serovars that produce typhoid-like infections is that disease-free persistent infection can occur for months or years in a small number of individuals post-convalescence. The bacteria continue to be shed intermittently which is a key component of the epidemiology of these infections. Persistent chronic infection occurs despite high levels of circulating specific IgG. We have reviewed the information on the basis for persistence in S. Typhi, S. Dublin, S. Gallinarum, S. Pullorum, S. Abortusovis and also S. Typhimurium in mice as a model of persistence. Persistence appears to occur in macrophages in the spleen and liver with shedding either from the gall bladder and gut or the reproductive tract. The involvement of host genetic background in defining persistence is clear from studies with the mouse but less so with human and poultry infections. There is increasing evidence that the organisms (i) modulate the host response away from the typical Th1-type response normally associated with immune clearance of an acute infection to Th2-type or an anti-inflammatory response, and that (ii) the bacteria modulate transformation of macrophage from M1 to M2 type. The bacterial factors involved in this are not yet fully understood. There are early indications that it might be possible to remodulate the response back towards a Th1 response by using cytokine therapy.

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High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

Cited by 10 publications

References 41 publications

Phenotypic and genotypic survey of antibiotic resistance in Salmonella enterica isolates from dairy farms in Uruguay

Phenotypic and genotypic survey of antibiotic resistance in Salmonella enterica isolates from dairy farms in Uruguay

Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella

Revisiting Persistent Salmonella Infection and the Carrier State: What Do We Know?

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