Advanced Optical Imaging Technologies II 2019
DOI: 10.1117/12.2536918
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High-speed in vitro intensity diffraction tomography

Abstract: We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume 3D refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60× to achieve highspeed 10 Hz volume rates. Using 8 intensity images, we recover ∼ 350 × 100 × 20µm 3 volumes with near diffraction-limited lateral resolution of 487 nm and axial resolution of 3… Show more

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Cited by 19 publications
(30 citation statements)
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References 68 publications
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“…These requirements make its practical implementation relatively complicated and challenging. Several recent techniques have demonstrated DT without a reference beam, instead using intensityonly images and a suitable phase retrieval algorithm for 3D sample reconstruction [8][9][10][11][12][13][14]. These methods, which effectively extend Fourier ptychography (FP) techniques into the third dimension [15], remove the need for a highly coherent beam and interferometric stability.…”
Section: Introductionmentioning
confidence: 99%
“…These requirements make its practical implementation relatively complicated and challenging. Several recent techniques have demonstrated DT without a reference beam, instead using intensityonly images and a suitable phase retrieval algorithm for 3D sample reconstruction [8][9][10][11][12][13][14]. These methods, which effectively extend Fourier ptychography (FP) techniques into the third dimension [15], remove the need for a highly coherent beam and interferometric stability.…”
Section: Introductionmentioning
confidence: 99%
“…Increased resolution/SBP Aperture synthesis [1,[21][22][23][24] Phase imaging Phase retrieval [25][26][27][28][29] Digital refocusing Phase retrieval [1,27,30] Aberration correction EPRY [24,[31][32][33] Long working distance low NA objective [34,35] Sub-λ imaging high-angle illumination [21][22][23]34,36,37] Multimodal imaging scanning illumination angle [24,34,38] High-speed LED & camera multiplexing [26,[39][40][41][42][43][44][45] Compact and portable Novel hardware [33,46,47] 3D imaging light field, 1st Born, multislice [48][49][50][51][52][53][54][55][56] computation using low numerical aperture (NA) op...…”
Section: Achieved By Referencesmentioning
confidence: 99%
“…We changed the matrix with a LED ring (Adafruit#1463) to demonstrate computational refocussing of a recovered phase map of cheek cells (Fig. 3 k)) by applying the "Annular Intensity Diffraction Tomography" (aIDT, [47], see also Supp. Chapter 3) method.…”
Section: /15mentioning
confidence: 99%
“…Chapter 3). For the quantitative phase measurements based on the aIDT, we used the publicly available Matlab (The MathWorks, MA, USA) code from Li et al [47] with small modifications according to the optical system using the cellphone microscope (see Supp. Chapter 3).…”
Section: Image Analysis and Image Processingmentioning
confidence: 99%