High Speed Photography of Laser Induced Forward Transfer (LIFT) of Single and Double-layered Transfer Layers for Single Cell Transfer

Riester, D.

doi:10.2961/jlmn.2016.02.0010

Cited by 25 publications

(12 citation statements)

References 24 publications

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“…In addition to the parameters of laser exposure, the transfer process is also affected by the hydrogel parameters (viscosity, density, and surface tension)[ 16 , 33 ] and its thickness on the ribbon. The variation of these parameters allows changing the jetting dynamics and droplet formation[ 15 ] and achieving the optimal transfer regime with the formation of a stable jet without spraying[ 14 ], the formation of hydrogel droplets of the required volume[ 15 ], and the absence of unnecessary shock stresses on the transferred objects[ 13 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Yusupov¹,

Churbanov²,

Churbanova³

et al. 2024

IJB

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Laser-induced forward transfer is a versatile, non-contact, and nozzle-free printing technique which has demonstrated high potential for different printing applications with high resolution. In this article, three most widely used hydrogels in bioprinting (2% hyaluronic acid sodium salt, 1% methylcellulose, and 1% sodium alginate) were used to study laser printing processes. For this purpose, the authors applied a laser system based on a pulsed infrared laser (1064 nm wavelength, 8 ns pulse duration, 1 – 5 J/cm2 laser fluence, and 30 μm laser spot size). A high-speed shooting showed that the increase in fluence caused a sequential change in the transfer regimes: No transfer regime, optimal jetting regime with a single droplet transfer, high speed regime, turbulent regime, and plume regime. It was demonstrated that in the optimal jetting regime, which led to printing with single droplets, the size and volume of droplets transferred to the acceptor slide increased almost linearly with the increase of laser fluence. It was also shown that the maintenance of a stable temperature (±2°C) allowed for neglecting the temperature-induced viscosity change of hydrogels. It was determined that under room conditions (20°C, humidity 50%), the hydrogel layer, due to drying processes, decreased with a speed of about 8 μm/min, which could lead to a temporal variation of the transfer process parameters. The authors developed a practical algorithm that allowed quick configuration of the laser printing process on an applied experimental setup. The configuration is provided by the change of the easily tunable parameters: Laser pulse energy, laser spot size, the distance between the donor ribbon and acceptor plate, as well as the thickness of the hydrogel layer on the donor ribbon slide.

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Section: Introductionmentioning

confidence: 99%

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Yusupov¹,

Churbanov²,

Churbanova³

et al. 2024

IJB

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“…In addition, various other cell types, both carcinomatous and normal, show high viability, irrespective of the laser absorbing layer utilized [ 33 , 45 , 46 , 47 ]. Following printing, cell viability remains between 80 to 90%, or even close to 100% [ 20 , 44 , 48 , 49 , 50 ]. Moreover, all these studies show that the cells recover and start to proliferate normally within a short period of time.…”

Section: Introductionmentioning

confidence: 99%

Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study

et al. 2022

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Laser-based techniques for printing cells onto different substrates with high precision and resolution present unique opportunities for contributing to a wide range of biomedical applications, including tissue engineering. In this study, laser-induced forward transfer (LIFT) printing was employed to rapidly and accurately deposit patterns of cancer cells in a non-contact manner, using two different wavelengths, 532 and 355 nm. To evaluate the effect of LIFT on the printed cells, their growth and DNA damage profiles were assessed and evaluated quantitatively over several days. The damaging effect of LIFT-printing was thoroughly investigated, for the first time at a single cell level, by counting individual double strand breaks (DSB). Overall, we found that LIFT was able to safely print patterns of breast cancer cells with high viability with little or no heat or shear damage to the cells, as indicated by unperturbed growth and negligible gross DNA damage.

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“…During LIFT printing, three jetting regimes are usually described: the subthreshold, well-defined jetting, and plume regimes. It has also been demonstrated that the cell-printing deposition and the cell viability depend on jet dynamics and the jet impact with the receiver substrate, which are controlled by the rheological properties (viscosity, density, and surface tension) [32,33], and by the distance between the donor and the receiving substrate [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink

et al. 2021

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Bioprinting offers great potential for the fabrication of three-dimensional living tissues by the precise layer-by-layer printing of biological materials, including living cells and cell-laden hydrogels. The laser-induced forward transfer (LIFT) of cell-laden bioinks is one of the most promising laser-printing technologies enabling biofabrication. However, for it to be a viable bioprinting technology, bioink printability must be carefully examined. In this study, we used a time-resolved imaging system to study the cell-laden bioink droplet formation process in terms of the droplet size, velocity, and traveling distance. For this purpose, the bioinks were prepared using breast cancer cells with different cell concentrations to evaluate the effect of the cell concentration on the droplet formation process and the survival of the cells after printing. These bioinks were compared with cell-free bioinks under the same printing conditions to understand the effect of the particle physical properties on the droplet formation procedure. The morphology of the printed droplets indicated that it is possible to print uniform droplets for a wide range of cell concentrations. Overall, it is concluded that the laser fluence and the distance of the donor–receiver substrates play an important role in the printing impingement type; consequently, a careful adjustment of these parameters can lead to high-quality printing.

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High Speed Photography of Laser Induced Forward Transfer (LIFT) of Single and Double-layered Transfer Layers for Single Cell Transfer

Cited by 25 publications

References 24 publications

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study

Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink

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