High‐speed separation and detection of amino acids in laver using a short capillary electrophoresis system

Wang, Wei; Ma, Lihong; Yao, Fen-Zeng; Lin, Xiuli; Xu, Kaixuan

doi:10.1002/elps.201400246

Cited by 16 publications

(12 citation statements)

References 17 publications

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“…The RSDs for the peak height and migration time were 2.12 and 0.82% ( n = 10) respectively. The result of migration time was similar to that of our previous report , while the repeatability for peak heights was improved obviously. The average number of theoretical plates for the FITC was 41 000 (corresponding to 1.83 μm plate height), which indicated that the spontaneous injection system could result in high separation efficiency for CE.…”

Section: Resultssupporting

confidence: 88%

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

et al. 2017

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A method using high-speed capillary micellar electrokinetic chromatography and a microbial fuel cell was applied to determine the metabolite of the peptides released by Bacillus licheniformis. Two peptides, l-carnosine and l-alanyl-l-glutamine were used as the substrate to feed Bacillus licheniformis in a microbial fuel cell. The metabolism process of the bacterium was monitored by analyzing the voltage outputs of the microbial fuel cell. A home-made spontaneous injection device was applied to perform high-speed capillary micellar electrokinetic chromatography. Under the optimized conditions, tryptophan, glycine, valine, tyrosine and the two peptides could be rapidly separated within 2.5 min with micellar electrokinetic chromatography mode. Then the method was applied to analyze the solutions sampled from the microbial fuel cell. After 92 h running, valine, as the metabolite, was successfully detected with concentration 3.90 × 10 M. The results demonstrated that Bacillus licheniformis could convert l-carnosine and l-alanyl-l-glutamine into valine. The method employed in this work was proved to have great potential in analysis of metabolites, such as amino acids, for microorganisms.

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Section: Resultssupporting

confidence: 88%

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

et al. 2017

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“…Nowadays, an increasing number of researchers perform CE separations in short capillaries (units of centimeters) instead of microfluidic chips [48,49]. In such capillaries, fast and efficient separations are carried out without complicated chip preparation requiring expensive facilities (e.g.…”

Section: Enhancement Of Separationmentioning

confidence: 99%

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

Adam¹,

Vaculovičová²

2018

Novel Nanomaterials - Synthesis and Applications

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Nanomaterials are attracting an interest of many researches. All this attention is due the unique physical and chemical properties of nanomaterials differing significantly from the bulk materials mainly due to their size in range of nanometers. Capillary electrophoresis (CE) is a powerful, well-established analytical technique that provides numerous valuable benefits over other separation methods including high-performance liquid chromatography. The connection between CE and nanotechnology can be approached by two strategies: (i) CE analysis of nanomaterials and (ii) nanomaterials for CE improvement. The first perspective focuses on uses of CE as a method for characterization employed during nanomaterial production and modification as well as for monitoring their properties and interactions with other molecules. The second viewpoint deals with applications of nanomaterials for improving CE performance, mainly by enhancing efficiency of separation using nanomaterials as a stationary or pseudo-stationary phase and by enhancing detection sensitivity and/or selectivity in both optical and electrochemical detection. Moreover, applications of nanomaterials for sample preparation before CE analysis will be mentioned. This chapter aims at highlighting the symbiosis of CE and nanotechnology as a combination of modern, progressive field with well-known and reliable analytical method.

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“…They also used this approach to search compounds that have potential modulating effects on BoNT/A activity. Even amino acids in natural products can be detected by LIF with little amount of injected sample . With high sensitivity, LIF can be used for detecting low content or even trace active ingredients in natural products.…”

Section: Screening Active Compounds Using Cementioning

confidence: 99%

Screening bioactive compounds from natural product and its preparations using capillary electrophoresis

Bai

et al. 2017

Electrophoresis

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Screening bioactive compounds from natural product and its preparations using capillary electrophoresisBioactive ingredients of natural products can protect human body from harm, as well as prevent and treat disease. Screening bioactive compounds from natural products is attracting particular attention. It is a great challenge to separate and detect the active compounds from complex matrix natural products. CE plays a vital role in screening active compounds because of its unique features such as high-efficiency separation, short-analysis time, minimal sample consumption, and ease to realize automatization etc. Additionally, CE has been developed various modes owing to its abundant advantages in analysis and separation of compounds. The purpose of this work is to review previous developments and applications of CE in screening bioactive compounds derived from natural products from 2007 to 2017. This review does not only summarize the traditional methods of detecting active ingredients but also briefly introduces some novel assays. The trends in the application of CE in active compounds screening are addressed in the article.

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High‐speed separation and detection of amino acids in laver using a short capillary electrophoresis system

Cited by 16 publications

References 17 publications

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

Screening bioactive compounds from natural product and its preparations using capillary electrophoresis

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